Cldn8 (BC003868) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI648025
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC003868 GGAGAACTGTTCCCTCCCTCCCAAGGCGTACAGATTTCTGCTTGAAAGGAAGCCCTACCTCCCGGGTCAG CGTTCCAGCTGGATACAATTTGGGAGGACGATGGCAACCTACGCTCTTCAAATGGCTGCACTGGTGCTTG GTGGTGTTGGCATGGTGGGCACGGTGGCTGTGACTATCATGCCTCAGTGGAGAGTGTCTGCCTTCATCGA AAGTAACATTGTGGTGTTTGAGAACCGCTGGGAAGGCTTGTGGATGAATTGTATGAGGCATGCCAACATC AGAATGCAGTGCAAGGTCTACGACTCCCTGCTGGCTCTTAGTCCAGACCTCCAGGCATCCCGAGGACTGA TGTGTGCTGCGTCCGTCTTGGCTTTCTTGGCTTTCATGACAGCCATCCTCGGAATGAAGTGCACCAGATG CACGGGGGACGATGAGAACGTGAAGAGCCGCATCTTGCTGACAGCCGGAATCATCTTCTTCATCACCGGC TTGGTTGTGCTCATCCCTGTCAGCTGGGTTGCCAATTCCATCATCAGAGACTTCTACAACCCACTGGTGG ATGTGGCCCTAAAGCGTGAGCTGGGAGAAGCCCTCTACATAGGCTGGACCACAGCGCTGGTGCTGATCGC TGGAGGAGCACTGTTCTGTTGTGTGTTTTGTTGTACTGAAAGGAGCAACAGTTACAGGTACTCGGTACCA TCCCATCGCACCACTCAACGGAGTTTCCACGCCGAAAAGAGATCTCCGAGCATATACTCCAAAAGTCAGT ATGTGTAGTTGTGTGTGTATATTTTAGCCGTAGCTAAGCTTTTATAAATCACCAAACCAGCCACTATTTT AGAGAAATGGAGCCCACACTGTATCCATTTACCTTTCTTAACTGCCTAGCACTCATTAGAGGAAATATGC AGCGTCTATGTTTCCATAAGTGATTTTAGCAGAATGAGGTATTATGTGCACTGCTTCGATTGTTCTAGAA AGCCTAGCCATTTGTTTTCTAAAATGGTTCACTCAGCTCTTCCTTTTTATCAGTCACTCTAAACCTGCAC ATCGAAAGACTGTTGGCTTAAGCTGTGATTTCTCTGTGTCATGACATTATCCAAGTAGATGCGGGTGGCA GTTATATCTCACACATATAGAGACAGGCTTATGTGGTTCTATTTAAAGTGGAATGCCAATCCATCACGCT GAATAAATAGAACTCAGCTATTGCATTTCAGGGGGCCTGGGGATAAAAGAGAAGGAGGCTGATATTAATT GCCTAAAAACAGCTTAAGGATTAGTGCACTCTATTTGTAGTGAAGGTTAAAATGAAGGCTTTAATCATTA GTGTAAGGCTTTCTGCTATCCTGCTTTGCAGCCTCGAGGAGTAAGAAATTCTGTCACCTTTATCCTTTCC CTCTACACGCCTTAATTTTCTGTTGCCTACTATCTCTTAAAGTTTAAAGCAGATGTTTTGGCAAAATATT TTTTTTCCTTTAGACTACTTTTGTAAGGCTAAGCAGTGGTCTTAGAAAAATTAATGATTTCAGAAAAATG AAATCATTAGCTTTTCTGTTTAGAGAAGAATGTATTGTTTTTGAAATGAAGTCATTTCATGTAAGGGATT GTTCAAATGCTATTTTTGCATAGTTTTTTCTTTCTTTTTTTTAGGAACTTATCAGTATATATGAGGGAGA AAGAAGGACATTTTCATTGTCATTTTATTAACCCAAAGAGAGACACAAACCCTCCAAATCCAAAAGCTTG TCCCCTTCTAACTCCTGACACTCAAACTTATCATTTCTCTTCTGTTGAAGGGATTTCCCCATTTTCTATG CCTTTGCTAAGTTCTATTGAAGTGTAGTATCACTCTGTGTTTCCTCCTAATTTGAGACAACTGTTATTCT AAGTTTCATATATATGACTTTTATATTGATAAATTGTATCTCATAACAATATAGCATCTCTTACATCTTC ATTCTTTTTCTTTACCTTGTGCAAGTGTTTAAAACTTGGTATTGACTCTTTTAGTTTGAACATAGATATT GTGTCCACTTTTGTAATGTATGATTTAAAATCCACGTACTTTATAATGACCTCACATATGCACTAATATT TGATTAATATATGAATAATATTAAATTGCACTTAGGTCCAATGAAATGTGTTTGTTGTAAATACTGTGTA TGTAATCATGTTTTAAAAGATGTTGTCCATATGTATTTCAACTCCTGAGAATTCATATGAACTAAGAGCA GTGCTGAATGACTTGGAAATATACATCATATTTATGTGAAGGATATTATTAAACGTTTGCAATACTAAAA AAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC003868
Insert Size	678 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC003868, AAH03868
RefSeq Size	2325 bp
RefSeq ORF	678 bp
Locus ID	54420
Gene Summary	This intronless gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The protein encoded by this gene is a paracellular cation barrier. [provided by RefSeq, Aug 2010]

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