Setd7 (BC050190) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1600028F23Rik; H3K4MT; KMT7; mKIAA1717; Set7; Set7/9
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC050190 CTTAGCTCCAGCGCTCGCTCAGCCACCAGGAGCACCCAGAGGGACGGGAGCCGGCCGCACGGCCCCGGGC TGGGTAGGGTCTCAGCCGCCATGGATAGCGACGATGAGGTGGTGGAGGAAGCCGTGGAAGGGCACCTGGA CGATGACGGATTACCACACGGGTTCTGCACTGTCACCTACTCCTCCACGGACAGATTTGAGGGCAACTTT GTGCATGGAGAAAAGAATGGGCGTGGGAAATTCTTCTTCTTTGACGGCAGCACCCTGGAGGGATATTACG TGGACGATGCCCTGCAGGGCCAGGGCGTTTACACCTATGTGGATGGAGGTGTTCTCCAAGGCACCTATGT GGATGGAGAGCTGAATGGACCGGCCCAGGAGTACGACTCTGACGGGAGGCTGATCTTCAAGGGACAGTAC AAAGACAACAATCGACACGGAGTGTGTTGGATCCATTACCCGGATGGAGGCAGCCTCGTCGGGGAAGTCA ATGAAGATGGAGAGATGACGGGAGAAAAGATCGCCTATGTGTACCCTGACCAGAGGACTGCGCTCTATGG GAAGTTCATTGACGGAGAGATGCTCGAAGGGAAACTGGCCACCCTCATGGCCACCGAAGAAGGGAGGCCA CACTTTGAAGTGACGTCGGGAAGTTCCGTGTACCACTTTGATAAATCGACTTCCTCCTGCATCTCCAGCG ATGCTCTCCTTCCAGACCCTTATGAGTCTGAGAGGGTTTATGTTGCCGACTCTCTCATCTCCAGTGCTGG AGAAGGGCTGTTTTCCAAGGTAGCAGTTGGACCTAATACTGTTATGTCGTTTTATAATGGAGTCCGAATT ACACACCAAGAGGTTGACAGCAGGGATTGGGCCCTAAATGGGAACACTCTCTCCCTGGACGAGGAGACAG TCATTGATGTGCCCGAGCCCTACAACCACGTGTCCAAGTACTGTGCCTCCTTGGGACACAAGGCCAATCA CTCCTTCACTCCGAACTGCGTCTATGACCTGTTTGTCCACCCCCGTTTCGGGCCGATCAAGTGCATCCGC ACCCTGCGAGCTGTGGAGGCCGAGGAAGAGCTGACCGTTGCCTACGGCTATGACCACAGCCCCCCGGGGA AGAGTGGGCCTGAAGCCCCCGAGTGGTATCAAGTGGAGCTGAAGGCCTTCCAGGCCACCCAGCAGAAGTG AAGGGCTTGGCACTGGGTCCCAGAGACCTGGAGTAGAAACTTGGATCTATGCACTACATTTATCTGACAA CGGGACAACCAGGGACTATTCATGCCATGACGTCACATCCTCTCACCCTGCGTTAGCAACGACTCTCTCT CTCTCTCTCTCTCTCTCTCTCCCCCCCCCCTCTCTCTCTCTCGCATACTAACTAGGTTTGACTGTATTAC TCGTACCAGATTTAAAATTAGCTAGCCTTGCCACAACGCCCTACTGAGAGGTACTGTCGAACTGTAGACA GCATGATGTTCTTTGATGGTGAAAGTCTAAATCTGGACCGTGTTCAGAGATACCAAATGATGAGGCTGAA AAGGGGAAACGGGGTTCTTCAGTCTCTTCTTCTTCTTTTTATTTTTTTTTCCATGATGTTTTCTCTATGG CCAGTGCAAATGGTGTTGTCACCCTTGCATGTTGCCAACCGCAGGGCAGGAAGCAATTACATCTTTATAT ACACGTAGGGTCATTTGCCTTTTAACCTTTGATCTGTATCTCTTGCAGTAGAATGGCTTTGTTTTTGTTG TTCCTCTCCTAGTGACCCGAAGCCCAGTGTTTTGAGTAATTTCTTTAGCTTCACTATACTAGCTATACTT TACAAGAGAAAGCTTCCACTTGGATTTTGCATTTGTAGCAGCGTACAGGTCTCTCTGGTCCTTTCTCTGC CATTTCACTCTTACGTCCTGATATAAAGTCCTGGCTCCTAGGGCCAGACTGTTATTTATAGACTTACTAT TCTCGCATGTAAACAAGGATATCTTTGCTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC050190
Insert Size	1101 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC050190, AAH50190
RefSeq Size	2022 bp
RefSeq ORF	1101 bp
Locus ID	73251
Gene Summary	Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation.[UniProtKB/Swiss-Prot Function]

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