Irak4 (BC051676) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	NY-REN-64, IRAK-4
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC051676 GTCCCACAGGGGGATATGATGCCCCCGTGACCCGCAGCATCCCGATCGCAGGCAGTCTGAAGTCGCCTGG TGGTCCTGCGTCCTCCACCCCCGAGTCCTCGCCGGACCGTGGCGGGACGCCGATCGCCTTGTCCAGGAAG CGAGGGACGTCCGAGAGGAAGTAGAAGATGAACAAGCCGTTGACACCATCGACATACATACGCAACCTTA ATGTGGGGATCCTTAGGAAGCTGTCGGATTTTATTGATCCTCAAGAAGGGTGGAAGAAATTAGCAGTAGC TATCAAAAAGCCGTCCGGCGACGACAGATACAATCAGTTCCATATAAGGAGATTCGAAGCCTTACTTCAG ACCGGGAAGAGCCCCACCTGTGAACTGCTGTTTGACTGGGGCACCACGAACTGCACAGTTGGCGACCTTG TGGATCTACTGGTCCAGATTGAGCTGTTTGCCCCCGCCACTCTCCTGCTGCCGGATGCCGTTCCCCAAAC CGTCAAAAGCCTGCCTCCTAGAGAAGCGGCAACAGTGGCACAAACACACGGGCCTTGTCAGGAAAAGGAC AGGACATCCGTAATGCCTATGCCGAAGCTAGAACACAGCTGCGAGCCACCGGACTCCTCAAGCCCAGACA ACAGAAGTGTAGAGTCCAGCGACACTCGGTTCCACAGCTTCTCGTTCCATGAACTGAAGAGCATCACAAA CAACTTCGACGAGCAACCCGCGTCTGCCGGTGGCAACCGGATGGGAGAGGGGGGATTTGGAGTGGTGTAC AAGGGCTGTGTGAACAACACCATCGTGGCGGTGAAGAAGCTCGGAGCGATGGTTGAAATCAGTACTGAAG AACTAAAGCAACAGTTTGATCAAGAAATTAAAGTAATGGCAACGTGTCAGCACGAGAACCTGGTGGAGCT GCTCGGCTTCTCCAGCGACAGCGACAACCTGTGCTTAGTGTATGCTTACATGCCCAACGGGTCCTTGCTG GACAGACTGTCCTGCCTGGATGGTACACCACCGCTTTCCTGGCACACAAGGTGCAAGGTTGCTCAGGGGA CAGCAAATGGCATCAGGTTTCTGCATGAAAATCATCACATTCATAGAGATATTAAAAGTGCAAATATCTT ACTAGACAGAGACTTTACTGCCAAAATATCTGACTTTGGGCTTGCACGGGCTTCGGCAAGGCTAGCGCAG ACGGTCATGACCAGCCGAATCGTGGGCACAACGGCTTACATGGCACCCGAAGCTTTGCGGGGAGAAATAA CACCCAAATCTGACATCTACAGCTTCGGCGTGGTTCTGTTGGAGCTGATAACCGGGCTGGCGGCTGTGGA TGAAAACCGTGAACCTCAACTACTGCTGGATATTAAAGAAGAGATTGAAGATGAAGAGAAGACGATTGAA GATTACACGGATGAGAAGATGAGCGATGCGGACCCTGCTTCGGTGGAAGCAATGTACTCTGCTGCTAGCC AGTGTCTGCATGAGAAGAAAAACAGACGGCCAGACATTGCAAAGGTTCAACAGCTGCTACAAGAGATGTC TGCTTAAAACTAATAAACTTTTTTTTTTTTTCTTTTTACTTCTCGAGAACCTGGAGACCGGAAAAGCACT TTGACACTGAGCTGCGTCACCTACCTGTTGTTTGCATTTGTTTTTGTTTTCCTAAATGCGCTTTATGTTT AACATGGTAGCATAGTGCAATGTACCAATGTACTGGTTAGTTAGTTAATAGACGAGTTGGCACCGCAGCC AAAGTGATAGTTGCATGGTAGCACTTGGCCCCGCCCACCTAGTATGGGGCTCATGTTCCACAGCAACATC ACCAAACTATAGTGAACTTAAGATTCAGGAAAACAGCACTGTGCTATACAGAGACAATAGGATTATTTGG GGCGGGGGGAGTGAGACACGGTGTCTCTGTGTAGCCCTGGATGTCCTGGAACTTGCTCTGTAGACCAGGC TGGCCTTGAACTCAGCAATCTGCCTGTCTCTGCCTACTGCGTGTTGGGATTAAAGGTGTGTGCCACTCCT GTAAAGCTTTGGACCAGCTCTTAGCTGCCTTAAGCCTTAGTGCTGGCTTTTGGTTTTTGATAGGAACTTC ATGCTCCTGTAAATAGCTCCTCACCTAGGTTCCTGTAAGGAACCTCTCTCCACTGCTCCTGTAACCCTAA CTAAACTCATTGGTTCAACAATGTAGACATGAGTGGAATCTATTCTTCTGTCTGTTATTGTGGTCCTATT TTGAATGAATAGATGTTTGCTTGTCATCCCTCCCCCCCCACCCCAATTCACATACATGGTACTATACGTG GGGTATAACAGAACCAATAGTGAGTTTGGAGGGTGTGTGTGTGTGTGCTTGTATTGTCTCTGCAATGGGC AATAAAACATGTAACTGAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC051676
Insert Size	1380 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC051676, AAH51676
RefSeq Size	2431 bp
RefSeq ORF	1380 bp
Locus ID	266632
Gene Summary	Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections (By similarity).[UniProtKB/Swiss-Prot Function]

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