Mbd4 (BC024812) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Med1
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC024812 GTTGATTGACTTTCACGTCGGTGTTTGGATTCGGCTCAGTGGTTTGAGTTGTGTTTTACTCCACCTCTCG GCCTGGAGCTGCAGCTGCGCCCCAGTGGACCAAAGCAGCAGGGATGGAGAGCCCAAACCTTGGGGACAAC AGAGTCCGTGGAGAGAGCCTAGTTCCAGACCCGCCTTGGGATCGCTGCAAGGAAGATATTGCTGTTGGAC TGGGAGGAGTGGGAGAAGATGGAAAGGACCTGGTGATAAGCAGTGAGCGCAGCTCCCTTCTCCAAGAGCC CACTGCTTCTACTCTGTCTAGTACTACAGCGACAGAAGGCCACAAGCCTGTCCCGTGTGGATGGGAAAGA GTTGTGAAGCAAAGGTTATCTGGGAAAACTGCAGGAAAATTTGATGTATACTTTATCAGCCCACAAGGAT TGAAGTTCAGATCAAAACGTTCACTTGCTAATTATCTTCTCAAAAATGGGGAGACTTTTCTTAAGCCTGA AGATTTTGATTTTACTGTACTGCCGAAAGGGAGCATCAATCCCGGTTATAAACACCAAAGTTTGGCAGCT CTGACTTCCCTGCAGCCAAATGAAACTGACGTTTCAAAGCAGAACCTCAAGACACGAAGCAAGTGGAAAA CAGATGTGTTGCCTCTGCCCAGTGGTACTTCAGAGTCGCCAGAAAGCAGCGGACTGTCTAACTCTAACTC GGCTTGCTTGCTATTGAGAGAACATAGGGACATTCAGGATGTTGACTCTGAGAAGAGGAGAAAGTCCAAA AGAAAGGTGACTGTTTTGAAAGGAACTGCAAGTCAGAAAACCAAACAAAAGTGCAGGAAGAGTCTCTTAG AGTCTACTCAAAGAAACAGAAAAAGAGCATCTGTGGTTCAGAAGGTGGGTGCTGATCGCGAGCTGGTGCC ACAGGAAAGTCAACTCAACAGAACCCTCTGCCCTGCAGATGCCTGTGCAAGGGAGACTGTTGGCCTGGCT GGGGAAGAAAAATCACCAAGCCCAGGACTGGATCTTTGTTTCATACAAGTAACTTCTGGCACCACAAACA AATTCCATTCAACTGAAGCAGCAGGTGAAGCAAATCGTGAGCAGACTTTTTTAGAATCAGAGGAAATCAG ATCGAAGGGAGACAGAAAGGGGGAGGCACATTTGCATACCGGTGTTTTACAGGATGGCTCTGAAATGCCC AGCTGCTCACAAGCCAAGAAACACTTTACTTCTGAGACATTTCAAGAAGACAGCATCCCACGGACACAAG TAGAAAAAAGGAAAACAAGCCTGTATTTTTCCAGCAAGTACAACAAAGAAGCTCTTAGCCCCCCAAGACG CAAATCCTTCAAGAAATGGACCCCTCCTCGGTCACCTTTTAATCTTGTTCAAGAAATACTTTTCCATGAC CCATGGAAGCTCCTCATCGCGACTATATTTCTCAATCGGACCTCAGGCAAGATGGCCATCCCTGTGCTGT GGGAGTTTCTAGAGAAGTACCCTTCAGCTGAAGTGGCCCGAGCTGCCGACTGGAGGGACGTGTCGGAGCT TCTCAAGCCTCTTGGTCTCTACGATCTCCGTGCAAAAACCATTATCAAGTTCTCAGATGAATATCTGACA AAGCAGTGGAGGTATCCGATTGAGCTTCATGGCATTGGTAAATATGGCAACGACTCCTACCGGATCTTTT GTGTCAATGAATGGAAGCAGGTGCACCCTGAAGACCACAAGTTAAATAAATACCATGACTGGCTTTGGGA GAATCATGAAAAATTAAGTCTATCTTGAAGCTGCCTGTTTGAAGTTTCCCATGCGTCGCCTTGTGCTTCG GTTTCTAAGGGCCCTGTGGTGTGCAGTGAGCTCCCTTCCCGGCACGTGGATTTTTTTTTTCAGTTTGTTA GAAGCCAGTGTATGTCTTAAGCCTCTGCCAAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC024812
Insert Size	1665 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC024812, AAH24812
RefSeq Size	1935 bp
RefSeq ORF	1665 bp
Locus ID	17193
Gene Summary	Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.[UniProtKB/Swiss-Prot Function]

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