Alcam (BC027280) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	CD166, DM-GRASP, MuSC, MGC27910, BEN, SC1
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC027280 CGTGGAGGAAAGTTGCGTGTGGCACAGACCCGACGGCGCAGCGCCACAGTCCCCGGGGGACCGTGTGTCT TGGAAAAAAGTCGCTGTCCCCCTAAGGCGAGACCCACAAGCGAGCGGGCCCCGTGGGCCCGGGGACGACG CCCCCTCCTGCGGCGTGGACTTTGTCGGTGGCCTTCTAGGAGGAGGAATATGGCATCTAAGGTGTCCCCT TCTTGCCGTCTGGTTTTCTGCCTCTTGATCTCCGCCGCGGTCCTCAGACCAGGCCTTGGATGGTACACTG TCAACTCAGCATATGGAGACACCATTGTCATGCCTTGCCGTCTCGATGTACCTCAGAACCTCATGTTTGG CAAATGGAAATATGAAAAGCCCGATGGGTCCCCAGTATTTATTGCATTCCGATCTTCTACGAAGAAAAGT GTGCAGTATGATGATGTCCCAGAGTACAAGGACAGACTGAGCCTCTCAGAAAACTACACTTTGTCTATCG CCAATGCTAAGATCAGTGATGAGAAGAGATTTGTGTGCATGCTAGTGACCGAGGACAATGTGTTTGAAGC ACCTACCCTAGTCAAGGTGTTCAAGCAACCATCTAAACCTGAAATTGTAAACAAAGCACCCTTTCTTGAA ACAGATCAGCTGAAAAAGTTAGGTGACTGCATTTCAAGAGACAGTTACCCAGATGGCAACATCACATGGT ACAGGAATGGCAAAGTGCTGCAGCCTGTGGAAGGAGAGGTGGCCATACTTTTTAAAAAGGAAATTGATCC AGGAACTCAGTTGTATACAGTGACTTCCTCCTTGGAGTACAAGACAACCAGATCTGACATACAAATGCCA TTCACCTGTTCTGTGACATATTATGGACCCTCCGGCCAGAAAACAATTTATTCCGAACAAGAAATCTTTG ATATTTACTATCCTACAGAGCAGGTGACAATACAAGTGCTGCCACCAAAAAATGCCATCAAAGAAGGGGA CAACATCACCCTTCAGTGTTTGGGGAATGGCAACCCACCTCCTGAGGAGTTCATGTTTTACTTACCAGGA CAGCCTGAAGGCATAAGAAGCTCAAACACTTACACACTGACAGATGTGAGACGCAATGCCACAGGGGACT ACAAATGCTCACTCATTGACAAAAGGAACATGGCGGCTTCAACGACCATCACAGTTCACTACTTGGATTT ATCCTTAAACCCAAGTGGGGAGGTGACCAAGCAGATTGGCGATACCCTGCCTGTGTCGTGCACAATATCT GCAAGTAGGAACGCGACTGTGGTGTGGATGAAGGATAACATCCGGCTCCGGTCCAGCCCTTCCTTTTCCA GTCTTCATTATCAGGATGCTGGGAACTATGTCTGTGAAACTGCTCTTCAGGAGGTTGAGGGACTGAAGAA AAGGGAGTCGCTGACACTCATTGTAGAAGGGAAACCTCAAATCAAAATGACTAAGAAAACTGACCCCAGT GGACTGTCTAAAACTATAATCTGCCACGTGGAAGGGTTTCCAAAGCCAGCTATACATTGGACCATTACCG GCAGTGGGAGCGTCATAAACCAAACAGAGGAGTCTCCTTATATTAATGGCAGGTATTATAGTAAAATTAT CATTTCCCCTGAGGAGAATGTTACATTAACTTGCACAGCAGAAAACCAACTGGAGAGAACAGTAAACTCC CTGAATGTCTCTGCGATAAGTATTCCAGAGCACGATGAGGCAGACGATATAAGTGATGAAAACAGAGAAA AGGTGAATGACCAGGCAAAACTGATTGTGGGAATTGTTGTTGGTCTCCTCCTCGCTGCCCTCGTTGCTGG TGTCGTCTACTGGCTGTACATGAAGAAGTCCAAAACTGCATCAAAACATGTAAACAAGGACCTTGGTAAT ATGGAGGAAAATAAAAAGCTGGAAGAAAACAATCACAAAACAGAAGCCTAAGAGAGGAGCGGATTGGATA GCCTGAGGAAAAAACATATAAGCAAATTGAAGCATGAACGTGGATTGTATTTAAGACATATACAAAGACA AATGACAGCAATTCATGGTTCAAGTATAAAGCAGTTCATTCTACCAAGCTGTCACAGGATCTTAGAGAAT TATCTCAAGTAAAACAGTGAAATAAAGTTATAAAATAAAATTACAAGATTTGGCAGCCATGATAATAGGT CATATGTTGTATTTCGTTTGGTTTTTTTTTCTGTAAATGTCTGCACTAAGGGTTTCTTTCAGTTTGCCTT TTATGTAAATTTTTTACGTAGCTATTTTTATACACTGTAAGCTTTGTTCTGGGAGTGGCTGTTAATTGGA TGTATAATGTAACGTTTTATTTCAATTGTTTATATGGATAGTCTGAGCAGGTACGGTTCTGATTCTGATT GCTATCAATAACACCCCATACTTTCTTTGAACATACTTTGACCTGAAGCTCAAAGGTGGCAGTTGTGAGA TTGGGACACATATCTTGCCCAATCAGAAGCTGTTTCTTATCTTGAGAGTTAGGAGCACCATAGGATATGA TGGTCTATGTGCTGCCAACAGGCTCCCCGAAGCGAGTCACAAACACATACAGCAACACTACTTGAGAATG GTACTCCCAAACTGACAATTTTTTCTTTTCTGAAAAAGATATACACATAGTGTGTATCACATTTACTTAT TGACACTTACTAAGAAATGACATTCTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC027280
Insert Size	1752 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC027280, AAH27280
RefSeq Size	2720 bp
RefSeq ORF	1752 bp
Locus ID	11658
Gene Summary	Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6 (By similarity). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (By similarity). Mediates homotypic interactions with cells that express ALCAM (PubMed:24740813). Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction. Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions (PubMed:23169771). Required for normal organization of the lymph vessel network (PubMed:23169771). Required for normal hematopoietic stem cell engraftment in the bone marrow (PubMed:24740813). Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow (PubMed:25730656). Promotes in vitro osteoblast proliferation and differentiation (PubMed:25730656). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM (By similarity). Mediates outgrowth and pathfinding for retinal ganglion cell axons (PubMed:15345243).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.