Agpat5 (BC031987) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110013A05Rik; D8Ertd319e
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC031987 GAGAGGATGCTGCTGTCCCTGGTGCTCCACACGTACTCTATGCGCTACCTGCTCCCCAGCGTCCTGTTGC TGGGCTCGGCGCCCACCTACCTGCTGGCCTGGACGCTGTGGCGGGTGCTCTCCGCGCTGATGCCCGCCCG CCTGTACCAGCGCGTGGACGACCGGCTTTACTGCGTCTACCAGAACATGGTGCTCTTCTTCTTCGAGAAC TACACCGGGGTCCAGATATTGCTATATGGAGATTTGCCAAAAAATAAAGAAAATGTAATATATCTAGCGA ATCATCAAAGCACAGTTGACTGGATTGTTGCGGACATGCTGGCTGCCAGACAGGATGCCCTAGGACATGT GCGCTACGTACTGAAAGACAAGTTAAAATGGCTTCCGCTGTATGGGTTCTACTTTGCTCAGCATGGAGGA ATTTATGTAAAACGAAGTGCCAAATTTAATGATAAAGAAATGAGAAGCAAGCTGCAGAGCTATGTGAACG CAGGAACACCAATGTATCTTGTGATTTTCCCAGAGGGAACAAGGTATAATGCAACATACACAAAACTCCT TTCAGCCAGTCAGGCATTTGCTGCTCAGCGCGGCCTTGCAGTATTAAAACACGTACTGACACCAAGAATA AAGGCCACTCACGTTGCTTTTGATTCTATGAAGAGTCATTTAGATGCAATTTATGATGTCACAGTGGTTT ATGAAGGGAATGAGAAAGGTTCAGGAAAATACTCAAATCCACCATCCATGACTGAGTTTCTCTGCAAACA GTGCCCAAAACTTCATATTCACTTTGATCGTATAGACAGAAATGAAGTTCCAGAGGAACAAGAACACATG AAAAAGTGGCTTCATGAGCGCTTTGAGATAAAAGATAGGTTGCTCATAGAGTTCTATGATTCACCAGATC CAGAAAGAAGAAACAAATTTCCTGGGAAAAGTGTTCATTCCAGACTAAGTGTGAAGAAGACTTTACCTTC AGTGTTGATCTTGGGGAGTTTGACTGCTGTCATGCTGATGACGGAGTCCGGAAGGAAACTCTACATGGGC ACCTGGTTGTATGGAACCCTCCTTGGCTGCCTGTGGTTTGTTATTAAAGCATAAGCAAGTAGCAGGCTGC AGTCACAGTCTCTTATTGATGGCTACACGTTGTATCACATTGTTTCCTGAATTAAATAAGGAGTTTTCTT GTTGTTGTTTTTTTGTTTTGTTTTGTTTTGTTCTGTTTTAAGCCTTGATGATTGAACACTGGATAAAGTA GAGTTTGTGACCACAGCCAACATGCATTTGATTTGGGGCAAACACATGTGGCTTTTCAGGTGCTGGGGTT GCTGGAGACATGGAAGCTAAGTGGAGTTTATGCTGTTTTTTTTTTTTTAATGTTTTCATGAATTAATGTC CACTTGTAAAGATTATTGGATACTTTCTGTAATTCAGAAGGTTGTATTTTAACAATAGTTTGCAGTATGT TTCGCTATATTGGTTATCTTCCATTTGACTACTTGGCAGCTCAGACTCTTAATACTAAAGTATTTTACAT TTTGAAGCTATGTGATACTGGTTTTTTGTTGTTGTTGTTGTTAATTTCTGAAAGTCAATGAAAGACACTG TAATGATGCGTTAAGATGTTCCAAGAAAAAGGTGAGAATTATTCATGGCAAAAAAGATCTGTCTAATGTA TATTTTTATTATATTGCTCTATTTAGCTAATTTTCTTTATATTTGCAAAATAATGAACATTTTTAATATT AAAATGCTTGATTTGCATACCCCCGATTCTACAGAGAATAATGTGTAAAGTGTCAGAATAGACTTGAAGC TCTGCTGTGACTCAGTCTCCTTTGTCAGAGCTTCTAGTAGCCCAGCTACTGAGCTGCTTTGTTAGTACCT CCAGCACCTGAGCCGTTAAGTACTTATAAATGCAAGGGACCCGTTATCTTCATATCGGAATAGACATGAA CAGAGCTCTAAGGCGATGAAAGTCTGCCAGCATCCTCTCTGTCCTCGCACGTGCCTTCTGCCTGGCTCCA TTTGCTTTGGCACTGCGTTCGATCTAGAGTGTAGGTGCTCACTGCTTATTTCAGCCCTGGCTCTGTGGTT TTGTGTCCTCCAGTGGTGCTGTTCACTGTTGGGGTGCAGGTGGTGCTGCCCTGACTCAGAGGGGCAGCTC CCTGGCTCCTGAGGGTGAGCCTTCTTGGCTACTACAGAAGTATTGTGCATTTGTTTATGGCAAGAACCAT CAGGATTGGATAAATGTGTTATTTCTCTTTGATTTCCATGGAGCCACACTGTTGGTACATGTCCCCTGTG AACAGAGCTACCTTTCAGGAGCACATCATACTGTCGTGAGTCACGGCACGGTGTGTCCTGTGAGAAGAGG CTTTCTAACGTGTGATTTGCCGTGTTTCTATGTTGTGATTTAAGCGTGATTGCCTACTAGTCATTCAAGG TAACATTTCTGCAAATTTCATACAGATTTTTTGTCACAAAATTACTATACCAATGATCTAGTTGAAATAG ACCAATTGAATCACAATAAATAATTTTTTTTAATTGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC031987
Insert Size	1098 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC031987, AAH31987
RefSeq Size	2628 bp
RefSeq ORF	1098 bp
Locus ID	52123
Gene Summary	Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (PubMed:15367102). Acts on LPA containing saturated or unsaturated fatty acids C15:0-C20:4 at the sn-1 position using C18:1-CoA as the acyl donor (By similarity). Also acts on lysophosphatidylethanolamine using oleoyl-CoA, but not arachidonoyl-CoA, and lysophosphatidylinositol using arachidonoyl-CoA, but not oleoyl-CoA (By similarity). Activity toward lysophosphatidylglycerol not detectable (By similarity).[UniProtKB/Swiss-Prot Function]

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