Ppil2 (BC028899) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1700016N17Rik, 4921520K19Rik, 4930511F14Rik, 0610009L05Rik
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC028899, the custom clone sequence may differ by one or more nucleotides ATGGGGAAGCGACAGCACCAGAAGGACAAGATGTATATCACTTGTGCTGAGTATACTCATTTCTATGGTG GCAGGAAGCCAGATATCTCACAGACAAGTTTTCGCCGATTACCTTTTGACCACTGCAGTCTCTCTCTCCA GCCTTTTGTCTACCCAGTCTGCACCCCAGAAGGTGTCGTCTTTGACTTGCTGAACATTGTTCCCTGGCTT AAGAAGTATGGGACGAATCCCAGCACTGGAGAGAAACTTGATGGGAAGTCCTTGATCAAGCTGAACTTCG CAAAGAACAGCGAAGGGCAGTACCACTGTCCAGTGCTGTATTCCGTGTTCACTGACAACACCCATATTGT GGCCATCAGGACAACTGGCAATGTCTACACCTATGAGGCAGTGGAGCAGCTAAACATCAAGGCCAAGAAC TTGCGGGATCTGTTGACCGATGAGCCCTTTTCCAGGCAAGACATCATCACCCTGCAGGACCCCACCAATT TGGACAAATTCAATGTTAGCAACTTCTTCCATGTGAAGAATAACATGAGAATAATAGACCCAGATGAGGA AAAGGCCAAACAAGACCCATCTTATTATTTGAAAAACACAAATTCGGAGACGAGAGAGACGCTACAGGAG CTCTACAAAGAGTTCAAAGGAGATGAGATTTTAGCAGCTACCATGAGGCCACCTGAGAAGAAGAAGGTGG ACCAACTGAATGCGGCCCACTACTCCACAGGGAAGGTCAGTGCATCCTTCACCTCTACTGCCATGGTGCC CGAGACCACGCATGAAGCAGCTGTCATTGATGAAGATGTACTGCGCTACCAGTTTGTGAAGAAAAAGGGC TATGTAAGGCTTCACACCAACAAGGGCGACCTTAACTTAGAGCTGCACTGTGACCTGACACCAAAAACCT GTGAAAACTTCATCAAGCTCTGCAAGAAACAGTATTATGATGGTACCATCTTTCACAGGTCCATCAGGAA CTTTGTGATCCAGGGCGGTGACCCCACAGGTACAGGCACAGGTGGAGAGTCATTCTGGGGCAAGCCTTTC AAAGATGAGTTCCGTCCCAACCTTTCACACACGGGCCGTGGGGTGCTCAGCATGGCCAATTCGGGGCCCA ACACCAACAAGTCTCAGTTCTTCATCACATTCCGATCCTGCGCTTACCTGGATAAGAAGCATACCATCTT TGGACGGGTTGTTGGGGGCTTTGACACGCTGACAGCCATGGAGAATGTGGAGAGTGACCCTAAAACTGAC CGTCCTAAGGAGGAAGTGCTCATATGTACAACCACAGTGTTTGTGGACCCCTATGAGGAGGCTGATGCCC AGATTGCCCAGGAACGGAAGAAGACACAGCATCAGGTGGATCCAGAGGCCAAAGTCAAGATGAGTCAGCC CCAGCCTGGAAACCAGGGACCCCAGACATACCGCCAGGGGGTGGGCAAGTACATCCACCCTGCGGCCACG AAACGATCAGCAGAGGAAGAACCATCGACCAGCACTGCCACCCCCACAGCCAAGAAAAGGCCCAGTCGGG GCTTTGGGGACTTCAGCTCCTGGTAG
Restriction Sites	EcoRI-NotI
ACCN	BC028899
Insert Size	1566 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC028899, AAH28899
RefSeq Size	2241 bp
RefSeq ORF	1566 bp
Locus ID	66053
Gene Summary	Has a ubiquitin-protein ligase activity acting as an E3 ubiquitin protein ligase or as an ubiquitin-ubiquitin ligase promoting elongation of ubiquitin chains on substrates. By mediating 'Lys-48'-linked polyubiquitination of proteins could target them for proteasomal degradation. May also function as a chaperone, playing a role in transport to the cell membrane of BSG/Basigin for instance. Probable inactive PPIase with no peptidyl-prolyl cis-trans isomerase activity.[UniProtKB/Swiss-Prot Function]

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