Mlf1 (BC027526) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	HLS7
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC027526 CAGGCTGGTGGCGGCGCAGAGAGCTGCGCCCATTTCCATCCGCGCCCGCCGCCCGCCGCAGGATCACGAT GTTCCGGATGCTGAGCAGCAGCTTTGAGGATGACCCCTTCTTCGCTGATTCTTTTCTTGCACACCGAGAA AGTATGCGCAACATGATGAGAAGTTTCTCTGAACCTCTTGGAAGAGACTTGCTCAGTATCTCTGATGGTA GAGGAAGAACCCATAATCGTCGAGAACGTGATGATGGCGAAGATTCCTTAACTGCAACAAGTTTTTCTCT TGTGCCTTTTGGAAGGTTTGGTGGAATGCATGCAGATGTCAACCCTTTTCAGACAATGGATCGGATGATG GCAAATATGCGAAGTGGTATACAGGAGTTACAAAGAAACTTTGGCCAACTTTCAATGGATCCAAATGGGC ATTCATTTTGTTCTTCCTCTGTTATGACCTATTCCAAAGTAGGAGATGAACCACCAAAGGTGTTCCAGGC CTCAACTCAAACCCGAAGGGCTCCAGGAGGAGTAAAAGAAACCAGAAAAGCAATGAGAGATTCTGACAGT GGACTAGAAAGAATGGCTGTTGGTCATCACATCCATGACCGAGGTCATGTCATTAGGAAGTCAAAGAACA ACAAGACTGGAGATGAAGAAGTCAACCAAGAGTTCATCAATATGAATGAAAGTGACGCTCATGCTTTTGA TGATGAGTGGCAAAATGAAGTTCTGAAGTACAAGTCTATTGGACGGTCAGGAAACACTGGAATGCGAAGT GTGGGTCATGAGCATCCAGGGTCACGGGAACTCAAAAGGAGAGAGAAAATTCATCGAAATTCAGCCATTG AGAGTGGAAGAAGATCAAACGTTTTTGTGGACAAACTCAATGTGAAAGGATCACCTGTGAAAATCACCAA AAAATAAATAGCCCTGCATTTCATTTGTTTAGTTGGTTATTTTAACAAAGTCATGGCATGGTGCTAGAAA ATACACAAAAGACCAATCTCCTGTTGTTAAATCAATGCTGTACTTTGCAACTTTCTTCCAAATATGAATG TTCTTGTAAGTTTTAGCTTTACATTGTTCATACTTTAGGAATAAAATATGGATGTTCAAAAAAAAAAAAA AAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC027526
Insert Size	849 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC027526, AAH27526
RefSeq Size	1126 bp
RefSeq ORF	849 bp
Locus ID	17349
Gene Summary	Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.[UniProtKB/Swiss-Prot Function]

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