Bok (BC030069) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	mtd, matador
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC030069 GCGGGTTTGAATGGAAGGGTCTAGACCGCCGGAGACGGCAGCGAGCGGGTCCTGAAACCAGAACTCCACC GCCGCCCCGCGCGCCATGAGGCGGGAGAGGTGTGGCGCCTCTCGCCCGCGCTTCGGCCATGGAGGTGCTG CGGCGCTCTTCTGTCTTTGCAGCGGAGATCATGGACGCCTTTGATCGCTCGCCCACAGACAAGGAGCTGG TGGCCCAGGCTAAGGCACTAGGCCGGGAGTACGTGCACGCGCGGCTTTTGCGCGCCGGCCTCTCCTGGAG CGCTCCAGAGCGTGCCTCGCCTGCCCCTGGAGGACGCTTGGCAGAGGTGTGCACAGTGCTGCTGCGCTTG GGAGATGAGCTGGAGCAGATCCGTCCCAGCGTATACCGGAACGTGGCCCGGCAGCTGCACATCCCCCTGC AGTCGGAGCCTGTGGTGACGGATGCCTTCCTGGCAGTGGCAGGCCACATCTTCTCAGCAGGTATCACATG GGGCAAGGTAGTGTCCCTGTATTCCGTGGCCGCGGGGCTAGCGGTGGACTGTGTCCGGCAGGCTCAGCCT GCCATGGTTCATGCCCTGGTTGACTGCCTGGGGGAATTTGTACGCAAGACCTTGGCTACCTGGCTTCGGA GGCGTGGCGGATGGACGGATGTCCTCAAGTGTGTGGTCAGCACAGATCCTGGCTTCCGCTCCCACTGGCT CGTGGCCACGCTCTGCAGCTTTGGCCGCTTCCTGAAGGCAGCATTCTTCCTGTTGTTGCCAGAGAGATGA GCTGGCCACCAGGGCAGGGGCCACTCCTAGGGTCCCTGGGCCCAATCCAAGGGGCCTCCAGTACCTACAA AGCACCTCCCTCACTCAAATTGGGAGCATTTAGCCCCTGGGGCCCTGTCCCAAACCCATTCCTTTGTGGA CCCTGGCCTCTGAGAGAGGAGTGTGGAGAAAGCCAGAGTCTGGAGCTGGCCTCTGTGACTGCTCTGGCTC TTCTCCTGGAACTCCTGCCAGAAAGTCAGGGTCGGTGTCCAGCCCTAGAGAAAGGGACCTGTGAATACTT TCACTCGATTTCCCAGGCCCCACCCAAGGAAGGGGCCTTCCCCAGACATGAGCTGGCCTCAGTCTTTGTG GAAAGCAGGTCCTGTACATTTGACCTGAAGGGGACTCTGGCTAATGCAGGAGAAGCCAGGGATGCAGAGT AAGAGAGCTTCTTGCTTAGGCTATTTCTAGATGAAGAAGGTATCCCTAAGCCACGATGGGTCCTTCATGG CAGGAACCAGAGAGGTAGGGCTCTAGGCTAGTTCACCTGACCAATGAACAAGAGATCCTGTGGATGAGGG GGTCTGTATAAGTTATACTCCAATAAAGCTTTACCTAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAA
Restriction Sites	EcoRI-NotI
ACCN	BC030069
Insert Size	642 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC030069, AAH30069
RefSeq Size	1403 bp
RefSeq ORF	642 bp
Locus ID	51800
Gene Summary	Apoptosis regulator that functions through different apoptotic signaling pathways (PubMed:23429263, PubMed:26015568, PubMed:26949185, PubMed:27098698, PubMed:9535847). Plays a roles as pro-apoptotic protein that positively regulates intrinsic apoptotic process in a BAX- and BAK1-dependent manner or in a BAX- and BAK1-independent manner (PubMed:23429263, PubMed:26015568, PubMed:26949185). In response to endoplasmic reticulum stress promotes mitochondrial apoptosis through downstream BAX/BAK1 activation and positive regulation of PERK-mediated unfolded protein response (PubMed:26015568). Activates apoptosis independently of heterodimerization with survival-promoting BCL2 and BCL2L1 through induction of mitochondrial outer membrane permeabilization, in a BAX- and BAK1-independent manner, in response to inhibition of ERAD-proteasome degradation system, resulting in cytochrome c release (PubMed:9535847, PubMed:26949185). In response to DNA damage, mediates intrinsic apoptotic process in a TP53-dependent manner. Plays a role in granulosa cell apoptosis by CASP3 activation (By similarity). Plays a roles as anti-apoptotic protein during neuronal apoptotic process, by negatively regulating poly ADP-ribose polymerase-dependent cell death through regulation of neuronal calcium homeostasis and mitochondrial bioenergetics in response to NMDA excitation (PubMed:27098698). In addition to its role in apoptosis, may regulate trophoblast cell proliferation during the early stages of placental development, by acting on G1/S transition through regulation of CCNE1 expression.May also play a role as an inducer of autophagy by disrupting interaction between MCL1 and BECN1 (By similarity).[UniProtKB/Swiss-Prot Function]

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