Tmem47 (BC019751) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	3010015F07Rik; AI115536; BCMP1; Tm4sf10
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC019751 CAGCAGCGGACGACCTCCTTAGGGCCAGGCGTCCCAGCTAGGCAAGTCCAGTTGACCACTCGGTGTGCTT AACTGAGACCCAAGGTACGACTTCTTGGCGCGGTGTAGCGCCGCTTTCCTGTTCGGGAGGTCCCGCTCCA GGCTCTTCGGACCCTCCTGGCGTCCTCGTTTGCGTCGGGAAGGGCGGCAGCGGCGCCCGGGCATGGCTTC GGCTGGCAGCGGCATGGAAGAGGTGCGAGTGTCGGTGCTGACCCCGCTCAAGCTGGTCGGGCTGGTGTGC ATCTTCTTGGCGCTGTGTCTGGATCTGGGAGCTGTTCTGAGCCCGGCCTGGGTCACCGCGGACCACCAGT ACTACCTGTCCCTGTGGGAGTCCTGCCGGAAACCCGCCAACCTGGATATCTGGCATTGCGAATCCACTCT CGGCAGCGATTGGCAGATCGCTACTCTGGCTTTACTCCTGGGCGGTGCTGCCATCATTCTCATTGCATTC CTGGTGGGTTTGATTTCAATCTGTGTGGGATCACGAAGGCGCTTCTACAGACCTGTTGCTGTCATGCTTT TTGCAGCAGTTGTTTTGCAGGTCTGCAGCCTGGTTCTTTATCCAATCAAGTTCATTGAAACTGTGAGCTT GAAAATTTACCACGAGTTCAATTGGGGTTATGGCCTGGCCTGGGGTGCAACTATATTTTCATTTGGGGGT GCCATTCTTTATTGCCTGAACCCTAAGAACTATGAAGACTACTACTAAAATCAAGTCTGAAAAACAAACA CACATATGCATACACACACACAAAACATACACTACACACACCACATTACACACAAACACACACACACACA CACACACACACACACCACATGAAAAAGAGAAACTAACAATCCAACAAAAGATTTGTGTTGACATCTTTTC TAACACTATTTTCTAAAACACTTGTGGAGCCTCAAGCAGTTTGCTCAGTTGATTTAATATCCTTGCCTTT GGGTGTCAACATCATAACCAGCTTTTATATCCATTTTAGGGACCTGTACAAATTAAGAATGCTGATTAGA TATAGGCATATGCTGCAAACTTCCAATATGCTTATGCCATTTCTCTTGACATGTGAAGGATCTGTATGAC AGCAACCATTTTGAGAAGCTAGTTGGAATGAGAATTGCCAGAGTCTCTTTTTGCCTGCAGGGAAACAGCT GCCTAAGCAACATGTAAAAGTTCCATTGCTCTGACATTTCTACTGTTGGAGCCCTTATCACCTGTTTATA TTGCGCAGTGGCTATATTGGTTATTGGTTACTTTGCTTTGGAATTTGAAACTGCCATTTGGGCATCTAAT GAGAATCATAGATATAGCCATTTAATTCTACCTTTAAGGCTGTTCTTTTTAATAGCAAATTATACTTTAC CAATTATCATCTCTTGGGAAGGAAGTCAACGTTTGGAGACATGTGTTTTTCATTATGTAGTTATAAAATT GAGGGTGTCTGCTTTCCCCTGGGGCAATCAGAAAGAAATGTGCATGAGTTGCTTTTGTACCCTTAAAATC CTTTAAAAATTTTGCAGCAAACAATTGTCCATATGTAACAAGCTTATTCTTCTACACCACAAGCCATTAC CATACTGGTAATAAGTTGCTCATTAATAGTCCACATACATGTACCTATCATTCATATAATCAATACTTGC TCTAGTGAAGCAATTTGAACACTAACCTTGTATGCATCAACAGACTCAGTATTCACAGTTACTTACCATA CAAAAGTTCATATACCTTAAAGCTTTTGCTCTGTGTTCTCTACTGATTCATTGGAAAGTATTAATAGAAT TGCCTAAGAAGTAAAATTGAAATGTTGTTAATCTGAAATTTAATGATTCTTTTATAAGTATTGTAGTTCA AAAGAGAGTAGGAATTAGAATGATCACTTTGTGTAAAATTCATTAAAATACAAGGCTGCTATTTTTGCAA GTATTTTAAATGTCTTCATTTTAAAATAAAGGAATAGTGATAGATTTCTAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC019751
Insert Size	546 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC019751, AAH19751
RefSeq Size	2024 bp
RefSeq ORF	546 bp
Locus ID	192216
Gene Summary	Regulates cell junction organization in epithelial cells. May play a role in the transition from adherens junction to tight junction assembly. May regulate F-actin polymerization required for tight junctional localization dynamics and affect the junctional localization of PARD6B (PubMed:26990309). During podocyte differentiation may negatively regulate activity of FYN and subsequently the abundance of nephrin (PubMed:21881001).[UniProtKB/Swiss-Prot Function]

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