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Eps8 (BC016890) Mouse Untagged Clone
CAT＃: MC206514
Eps8 (untagged) - Mouse epidermal growth factor receptor pathway substrate 8 (cDNA clone MGC:18488 IMAGE:4240899), (10ug)
CNY 6,024.00
货期*
现货
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AW261790 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC016890
GACCCACGCGTCCGGAGTTCTCCATCAATGCTTCCCATTGTTATTTGCTGCTATGTTAGTCTCCCCACCT CGGGGAAGGGCTTTACCTCCTCAGCGGTGGTGATTTCCTTGTTTAAAAGAGGAGGAAGTCTTAGCTGAGG CCTGGGAGAGTCCAAAGGAGAGACTTTGATGAGTATAGCGGAACACCGAAACTGAAGCAGTCCCACCCTC TCGGTGTATGAACCAAAGGTCACTGGACACATCCGTGAGCAGGGAGCGATGCTCTCTCAGTCTTGCCTTG ACAGTTGCTTGGACGAGTTTTACCTCAGCAGTGCAGGAAGAACAGAGGCGTGAGAGCCGGCATGAATGGT CATATGTCTAACCGCTCCAGTGGGTATGGAGTCTACCCTTCTCAACTGAATGGTTACGGATCTTCACCAC CCTATTCCCAGATGGACAGAGAACACAGCTCAAGAACAAGTGCAAAGGCCCTTTATGAACAAAGGAAGAA CTATGCCCGAGACAGTGTCAGCAGTGTGTCGGACGTGTCCCAGTACCGCGTGGAACACTTGACCACCTTC GTGCTGGATCGGAAAGATGCAATGATCACTGTCGAGGACGGAATAAGAAAGCTGAAGTTGCTGGATGCCA AGGGCAAAGTGTGGACTCAAGATATGATTCTCCAAGTGGATGACCGAGCTGTGAGCCTGATTGACTTAGA GTCAAAGAATGAATTGGAGAATTTTCCTCTAAACACAATCTCGCATTGTCAAGCAGTGGTGCATGCATGC AGCTATGACTCCATTCTCGCCTTGGTATGCAAAGAGCCAACGCAGAGCAAGCCAGACCTTCACCTTTTCC AGTGTGATGAGGTTAAGGCAAACCTAATTAGTGAAGATATCGAAAGTGCAATCAGTGACAGTAAAGGTGG GAAACAGAAGAGGCGGCCGGAGGCCCTGAGGATGATTGCCAAAGCAGATCCTGGCATCCCTCCTCCTCCC AGAGCTCCTGCCCCTGTGCCACCGGGGACTGTCACACAGGTGGACGTTAGGAGTCGCGTAGCAGCCTGGT CTGCCTGGGCAGCTGACCAGGGTGACTTCGAGAAGCCCCGGCAGTACCACGAGCAAGAAGAGACGCCCGA GATGATGGCAGCCCGGATCGACAGGGATGTGCAAATCTTAAACCATATTTTGGATGACATTGAATTTTTT ATCACCAAACTCCAAAAAGCCGCCGAAGCGTTTTCTGAGCTTTCTAAAAGGAAGAAAAGTAAGAAAAGTA AAAGGAAAGGACCTGGAGAGGGCGTTTTAACACTGAGGGCAAAACCGCCACCTCCTGACGAGTTTGTTGA CTGTTTCCAGAAGTTTAAACATGGATTCAACCTTCTGGCCAAGTTGAAGTCCCATATCCAGAACCCGAGT GCTTCAGATCTGGTTCATTTTTTGTTTACTCCACTAAATATGGTGGTCCAGGCAACAGGTGGCCCTGAAC TGGCCAGTTCGGTACTCAGCCCACTGTTGACAAAAGACACAGTTGATTTCTTAAACTACACAGCCACTGC GGAGGAACGGAAGCTGTGGATGTCACTGGGAGATAGTTGGGTGAAAGTGAGAGCAGAGTGGCCGAAAGAA CAGTTCATCCCACCTTACGTCCCGAGGTTCCGCAACGGCTGGGAGCCCCCGATGCTGAACTTCATGGGCG CGCCCACAGAGCAAGACATGTATCAACTGGCCGAGTCCGTGGCCAACGCAGAACACCAGCGCAAACAGGA CAGCAAGAGGCTGTCCACAGAGCATTCCAATGTGTCCGACTATCCTCCAGCCGACGGATATGCGTACAGT AGCAGCATGTACCACAGAGGACCACATGCAGACCACGGGGAGGCTGCCATGCCTTTCAAGTCAACTCCTA ATCACCAAGTAGATAGGAATTATGACGCAGTCAAAACACAACCCAAGAAATACGCCAAATCCAAGTACGA CTTTGTGGCGAGGAACAGCAGCGAGCTCTCGGTTATGAAAGATGATGTCTTAGAGATACTCGACGATCGA AGGCAGTGGTGGAAAGTCCGGAATGCCAGTGGAGACTCTGGGTTTGTGCCAAATAACATTCTGGATATCA TGAGAACTCCAGAATCTGGAGTGGGGCGCGCTGACCCCCCATACACACATACCATACAGAAACAAAGGAC GGAATACGGCCTGAGATCAGCTGACACTCCTTCTGCCCCATCACCCCCTCCAACGCCAGCACCCGTTCCG GTCCCCCTTCCACCTTCTGTACCAGCACCCGTTTCTGTGCCCAAGGTTCCAGCAGATGTCACCCGCCAGA ACAGCAGCTCCAGTGACAGTGGGGGCAGCATTGTGCGGGACAGCCAGAGATACAAACAACTCCCAGTGGA CCGAAGGAAGTCCCAGATGGAAGAGGTTCAGGATGAGCTCTTCCAGAGGCTGACCATCGGGCGCAGTGCT GCGCAGAGGAAGTTCCACGTGCCACGGCAGAACGTTCCAGTGATCAATATCACTTATGACTCCTCACCGG AAGAAGTAAAGACTTGGCTGCAGTCAAAGGGATTCAATCCCGTGACTGTCAATAGCCTCGGGGTGTTGAA CGGAGCACAACTCTTTTCTCTCAACAAAGACGAACTGAGGTCTGTCTGCCCGGAAGGTGCCAGAGTCTTT AACCAAATCACTGTTCAGAAAGCTGCTTTGGAGGACAGTAATGGAAGCTCCGAGTTACAAGAGATCATGC GGAGACGGCAGGAGAAGATCAGCGCCGCTGCGAGCGACTCGGGAGTGGAGTCTTTTGATGAAGGGAGCAG CCACTGAGTCCATGAACTTCCTTATTCTTGGTGTGGTCGTTGAACAGTGATGGACATGCTTTGTTTTAAG AAGCCTTGAAGGGAATGTCAAAGCTGTCGTCTTGGTATATGTAATTTATCGCCATATAAGGAAACAGTAT ATGCCTGAGTAAGCAGAGGACCCGCTGCTTCTGTGCACATTAGTTTGATTAAAACTGAGAAGCGGGTAGG TGAGATGGCTCAGCAAGTAAAGGTGCTTGCTGCCAAGCCCAATGACCCAAGTTCGAGTCCCTGGGTCTAC ATGGTAGGAGAGAGCTGGCTTCTGCAAGTTGTCCTCTGACCACCACACATAAATAAATAACAAATGTAAT TTACAAACTTTTAAAAAAAAAAAAAAA |
| Restriction Sites | EcoRI-NotI |
| ACCN | BC016890 |
| Insert Size | 2466 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC016890, AAH16890 |
| RefSeq Size | 3177 bp |
| RefSeq ORF | 2466 bp |
| Locus ID | 13860 |
| Gene Summary | Signaling adapter that controls various cellular protrusions by regulating actin cytoskeleton dynamics and architecture. Depending on its association with other signal transducers, can regulate different processes. Together with SOS1 and ABI1, forms a trimeric complex that participates in transduction of signals from Ras to Rac by activating the Rac-specific guanine nucleotide exchange factor (GEF) activity. Acts as a direct regulator of actin dynamics by binding actin filaments and has both barbed-end actin filament capping and actin bundling activities depending on the context. Displays barbed-end actin capping activity when associated with ABI1, thereby regulating actin-based motility process: capping activity is auto-inhibited and inhibition is relieved upon ABI1 interaction. Also shows actin bundling activity when associated with BAIAP2, enhancing BAIAP2-dependent membrane extensions and promoting filopodial protrusions. Involved in the regulation of processes such as axonal filopodia growth, stereocilia length, dendritic cell migration and cancer cell migration and invasion. Acts as a regulator of axonal filopodia formation in neurons: in the absence of neurotrophic factors, negatively regulates axonal filopodia formation via actin-capping activity. In contrast, it is phosphorylated in the presence of BDNF leading to inhibition of its actin-capping activity and stimulation of filopodia formation. Component of a complex with WHRN and MYO15A that localizes at stereocilia tips and is required for elongation of the stereocilia actin core. Indirectly involved in cell cycle progression; its degradation following ubiquitination being required during G2 phase to promote cell shape changes.[UniProtKB/Swiss-Prot Function] |
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