Acadm (BC013498) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AU018656; MCAD
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC013498, the custom clone sequence may differ by one or more nucleotides ATGGCAGCGGCGTTCCGCAGAGGCTGCAGGGTCCTGAGAAGTGTTTCTCATTTTGAGTGTCGAACACAAC ACTCGAAAGCGGCTCACAAGCAGGAGCCCGGATTAGGGTTTAGTTTTGAGTTGACGGAACAGCAGAAAGA GTTTCAAGCAACTGCCCGCAAGTTTGCCAGAGAGGAGATTATCCCCGTCGCCCCGGAATATGACAAAAGC GGGGAGTACCCGTTCCCTCTCATCAAAAGAGCCTGGGAACTCGGCTTGATCAACGCGCACATTCCGGAAA GTTGCGGTGGTCTTGGCCTGGGAACGTTCGATGCTTGTTTAATTACCGAAGAGTTGGCGTATGGGTGTAC AGGGGTGCAAACTGCTATTGAAGCAAATTCTTTGGGGCAAATGCCTGTGATTCTTGCTGGAAATGATCAA CAAAAAAAGAAGTATTTGGGGAGGATGACGGAGCAGCCAATGATGTGTGCTTACTGTGTGACAGAGCCCT CCGCAGGCTCTGATGTGGCGGCCATTAAGACCAAAGCAGAGAAGAAGGGTGACGAGTATGTTATCAACGG CCAGAAGATGTGGATAACCAACGGGGGAAAGGCCAACTGGTATTTCTTGTTGGCGCGTTCTAACCCAGAT CCTAAAGTACCCGCTAGTAAAGCCTTTACTGGATTCATTGTGGAAGCCGACACCCCGGGAATACATATTG GAAAAAAGGAACTAAACATGGGCCAGCGATGCTCTGACACCAGAGGAATTGCTTTCGAAGACGTCAGAGT GCCTAAGGAAAATGTGTTAATCGGTGAAGGAGCAGGTTTCAAGATCGCAATGGGTGCTTTTGATAGAACC AGACCTACAGTCGCAGCTGGCGCTGTCGGGCTAGCCCAGAGAGCTCTGGACGAAGCCACGAAGTATGCCC TGGATAGGAAGACATTTGGAAAGCTGCTAGTGGAGCACCAAGGAGTTTCATTTCTGCTCGCAGAAATGGC GATGAAGGTTGAACTCGCTAGGCTCAGTTACCAGAGAGCAGCCTGGGAGGTTGACTCCGGTCGCCGGAAC ACTTACTATGCCTCGATTGCAAAGGCCTGTGCTGGAGACATTGCCAATCAGCTAGCCACTGACGCCGTGC AGATTTTCGGAGGCTATGGATTCAACACAGAGTACCCTGTGGAGAAGCTGATGAGGGACGCCAAGATCTA TCAGATTTATGAAGGTACTGCACAAATTCAGAGGCTGATCATAGCTCGTGAGCACATTGAAAAGTATAAA AATTAA
Restriction Sites	EcoRI-NotI
ACCN	BC013498
Insert Size	1266 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC013498, AAH13498
RefSeq Size	1857 bp
RefSeq ORF	1266 bp
Locus ID	11364
Gene Summary	This gene encodes a homotetrameric mitochondrial flavoprotein and is a member of the acyl-CoA dehydrogenase family. Members of this family catalyze the first step of fatty acid beta-oxidation, forming a C2-C3 trans-double bond in a FAD-dependent reaction. As beta-oxidation cycles through its four steps, each member of the acyl-CoA dehydrogenase family works at an optimum fatty acid chain-length. This enzyme has its optimum length between C6- and C12-acylCoA. In mice, deficiency of this gene can cause neonatal mortality as well as fasting and cold intolerance. This gene has multiple, intronless pseudogenes. [provided by RefSeq, Nov 2012]

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