Vps4a (BC018368) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	MGC11698
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC018368, the custom clone sequence may differ by one or more nucleotides ATGACAACGTCAACCCTCCAGAAAGCCATCGATCTGGTGACAAAAGCCACAGAAGAGGATAAAGCCAAGA ACTACGAGGAGGCACTCCGTCTCTACCAGCATGCTGTGGAGTATTTCCTCCACGCCATCAAATATGAAGC ACACAGTGACAAGGCCAAGGAGAGCATTCGAGCAAAGTGCATGCAGTACCTAGACAGAGCGGAGAAGCTG AAGGATTATTTACGAAACAAAGAGAAACATGGCAAGAAGCCAGTCAAAGAGAACCAGAGTGAAGGCAAGG GTAGTGACAGTGACAGTGAAGGGGATAATCCAGAGAAAAAGAAGTTACAAGAACAATTGATGGGTGCTGT TGTGATGGAGAAGCCGAACATACGGTGGAATGATGTAGCTGGACTGGAGGGGGCTAAAGAGGCTCTCAAA GAAGCTGTGATTTTGCCAATTAAATTCCCACACTTATTCACAGGCAAACGCACTCCTTGGCGAGGCATAC TCCTCTTTGGGCCCCCAGGCACAGGCAAGTCCTACCTGGCCAAAGCCGTGGCAACAGAAGCCAACAACTC TACCTTCTTCTCTGTGTCCTCCTCGGATCTGATGTCTAAGTGGTTGGGGGAAAGCGAGAAGCTAGTCAAG AACCTGTTTGAGCTGGCCCGGCAGCATAAGCCTTCCATCATCTTCATTGATGAGGTAGATTCCCTCTGTG GGTCACGGAATGAAAATGAGAGTGAAGCCGCTCGTAGGATCAAAACAGAGTTCTTGGTCCAGATGCAAGG AGTGGGGAATAATAATGATGGAACTCTTGTTCTTGGTGCCACAAACATCCCCTGGGTGTTGGATTCAGCC ATCAGGAGGAGGTTTGAAAAGCGAATTTATATCCCATTGCCAGAGGAAGCGGCCCGTGCCCAAATGTTTC GGTTACATCTGGGAAGCACACCTCACAACCTCACAGATGCTAACATTCACGAGCTGGCCCGCAAGACAGA AGGCTACTCAGGAGCAGATATCAGCATCATTGTGCGGGACTCCCTCATGCAGCCAGTCAGAAAAGTACAG TCAGCAACACACTTCAAGAAGGTGTGTGGCCCTTCTCGAACCAACCCTAGCGTTATGATTGATGATCTTC TGACCCCATGTTCTCCGGGAGATCCAGGGGCCATAGAGATGACTTGGATGGATGTCCCTGGTGATAAACT CTTAGAGCCTGTGGTTTGCATGTCGGACATGCTCCGGTCTTTGGCCACTACCCGGCCCACAGTGAATGCA GACGACCTCCTGAAAGTAAAGAAATTCTCTGAGGACTTTGGACAGGAGAGTTAA
Restriction Sites	EcoRI-NotI
ACCN	BC018368
Insert Size	1314 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC018368, AAH18368
RefSeq Size	2089 bp
RefSeq ORF	1314 bp
Locus ID	116733
Gene Summary	Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission. VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (By similarity).[UniProtKB/Swiss-Prot Function]

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