Tspan12 (BC068240) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9030619E17; AI426782; AI663988; AW111457; Tm4sf12
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC068240 GGAAGACAAGGAAGCGTCTGCTGCTGGCGCCGATGCATCCCTTTGGCTCTCCGGGAGGAGCGTCCCTGCC CGGATGAGTGTCTGCTGTGTGTCCCCGACTGTTCCCCGGCAGGAGCGCAGCTCGAACGATCGCCGGGGCT CTGCCTGGACCAGTAGAATGTGGTGAGAGGATGGGGTTTCTCCCTCCCAAGACTCACAATGGCCAGAGAA GATTCCGTGAAGTGTTTGCGCTGCTTGCTCTACGCCCTCAACCTGCTCTTTTGGTTAATGTCCATCAGTG TCTTGGCAGTTTCTGCTTGGATGAGGGACTACCTGAATAATGTTTTGACTTTAACTGCAGAAACAAGGGT AGAAGAAGCGGTCATCTTAACTTACTTCCCCGTGGTTCACCCCGTCATGATTGCTGTCTGCTGCTTCCTT ATCATCGTGGGAATGCTGGGATACTGTGGAACGGTGAAAAGAAATCTGTTGCTTCTTGCATGGTACTTCG GAACGTTACTCGTCATCTTCTGTGTAGAACTGGCTTGCGGTGTGTGGACATACGAGCAGGAGGTTATGGT GCCAGTACAGTGGTCAGATATGGTTACTTTGAAAGCCAGAATGACAAATTACGGCTTACCGAGGTATAGA TGGCTTACACACGCTTGGAATTATTTTCAGAGAGAGTTTAAGTGCTGTGGAGTGGTGTACTTCACTGACT GGTTGGAAATGACAGAAATGGACTGGCCTCCCGACTCCTGCTGTGTCAGGGAGTTCCCAGGGTGCTCCAA GCAGGCTCACCAGGAAGATCTCAGTGACCTTTACCAAGAGGGTTGTGGGAAGAAGATGTATTCCTTTTTG AGAGGAACCAAACAATTGCAAGTTCTAAGGTTTCTGGGAATCTCCATTGGTGTGACACAAATCCTGGCCA TGATTCTCACCATTACTCTGCTCTGGGCTCTGTATTATGATAGAAGGGAGCCTGGAACAGACCAAATGCT ATCTCTGAAAAATGATACGTCTCAGCACTTGTCATGTCATTCTGTGGAACTGTTAAAACCAAGTCTTTCA AGGATCTTTGAGCATACATCAATGGCAAACAGCTTCAATACACATTTTGAGATGGAAGAATTATGAAGAA TGTCAAAGAAGGAAATCACAAACTTGTGGGACTAGTGGGTTTTGGGGTACACAGTTATCTATTTCAGAAA TTTTTAGACATATTAATATTCTGAAAAGGAATGTCTAAGAATTTCTCTACTCTTTAAAACAAAGGTTCCA CATGGTAATACACAGACAATAATTGGTGCCCTTCAAAATGCCGAACCCAGACATTATAACAACTGTGTGT GCTTTCCTTTCTTTTCTTTTTTTCTGAGCACAATTATGTTTTGCAGTGATACAGTTTGACCAGCGTTTCT GCATCCATTCACAGAAATGAACCGTCATGGTGGCACTGGAGCTGATATGAAGGTTAACTGACTCCTCTAA CTAACCTATAAACTATCCACTGACTGCTAACATAGGGATTTAGTGCTAAAGACCTTTATTAGCTGGTGAT GTATTGTTCTGATGAGGTATTTGATTATATGCAAAAATCTTTCAAGATTGGTGACTACCTAAATATGATT TTTGTTGGTTACTAAAATAATCTTATTAATAATACTTGTAAGAGCTAACATGTTGTCTTAAACTTATCAG GGATATATATGTATATAAGTCTGTGCCGTGTCTGTATAATTCAGTAGATTTCAGTTCCTATAATATTATG AAAAACAATATTTGAAAATGGCGAAAATTGTCTTACACAGCACCATTACTTTTGTCTCTTTCAGCAACAG AACTTTTAAATTCCGTCTTGGTTCTGTACTATATCTATAACTGTTAATTTAAGTTGTTAACCACTAATTT TGGAAATTACCAGTTTGACACATAGGAATCTCTGTAAGTCTGCCTGCAGGAAGTATTAACTTCAGAATTT GCACATAGTTGATTCATCAAAAGTTCAGATTTTTCCCCCTCCCAATAAACCCTTTGTCAAAGAGTGTTTT TCATTTTTATTTTTAATGTTTTCATGTATTTTTTTGCATTCAAGAATCAACAACTTGAAGTTGATGTAAT TTTCCAGAGAAACAACATTCTCTGAAGAGATGCTCATAGGAATCTCTAAAATAAGTGGTAGCCTAACCAG AGAGAATTCTTGATTTCTTTCATCAATTAATACACTGTAGCAAATGATACAGAATATTAATTTTTTACAA GAGTAGAAGATATTTATTTGAAACGGGAAAAGTGCATTTTACTGTATCTTGTGCATTCTGTTCATTTCTT GGATTACAGAAAGAAAATTAATCAATAAATATTTTCTAGATCGTGAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC068240
Insert Size	918 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC068240, AAH68240
RefSeq Size	2388 bp
RefSeq ORF	918 bp
Locus ID	269831
Gene Summary	Regulator of cell surface receptor signal transduction. Acts as a regulator of membrane proteinases such as ADAM10 and MMP14/MT1-MMP. Activates ADAM10-dependent cleavage activity of amyloid precursor protein (APP). Activates MMP14/MT1-MMP-dependent cleavage activity (By similarity). Plays a central role in retinal vascularization by regulating norrin (NDP) signal transduction. Acts in concert with norrin (NDP) to promote FZD4 multimerization and subsequent activation of FZD4, leading to promote accumulation of beta-catenin (CTNNB1) and stimulate LEF/TCF-mediated transcriptional programs. Suprisingly, it only activate the norrin (NDP)-dependent activation of FZD4, while it does not activate the Wnt-dependent activation of FZD4, suggesting the existence of a Wnt-independent signaling that also promote accumulation the beta-catenin (CTNNB1).[UniProtKB/Swiss-Prot Function]

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