Fus (BC058247) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Fus1, Tls
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC058247 GCATCGCTTCGCGGTCGTCTGGAACTTTGTTGCTTGGCGGCCTGCGCTGCGCGCGTGCGCGGACATGGCT TCAAACGACTATACCCAACAAGCAACTCAAAGCTACGGGGCCTATCCTACCCAGCCTGGGCAGGGCTACT CCCAACAGAGCAGTCAGCCCTATGGCCAACAGAGTTACAGTGGTTATGGCCAGTCAGCTGACACTTCAGG ATACGGCCAGAGCAGCTATGGTTCTTCTTATGGACAGACCCAAAACAGCTATGGAACTCAGTCAGCTCCC CAGGGATATGGTTCCACTGGAGGCTATGGCAGCAGCCAAAGTTCACAATCTTCTTATGGGCAGCAGTCCT CCTACCCTGGCTATGGCCAACAGCCAGCTCCTAGCAGCACCTCAGGAAGTTATGGTGGCAGTTCTCAGAG CAGCAGCTATGGGCAACCCCAGAGTGGAGGTTATGGTCAACAGTCTGGCTATGGTGGACAGCAACAAAGC TACGGACAACAACAAAGCTCCTATAACCCACCTCAGGGTTATGGACAACAGAACCAGTACAACAGTAGCA GTGGAGGTGGTGGAGGGGGTGGTGGAGGCAACTATGGCCAAGATCAGTCCTCTATGAGTGGTGGCGGTGG CGGTGGTGGTTATGGCAATCAGGACCAGAGTGGTGGCGGCGGCGGCGGCTACGGGGGAGGCCAACAGGAT CGTGGGGGCCGAGGCAGGGGCGGTGGAGGTGGTTACAACCGAAGCAGTGGTGGCTATGAACCCAGAGGCC GTGGAGGTGGCCGAGGAGGCAGAGGCGGCATGGGCGGAAGTGACCGCGGTGGCTTCAATAAATTTGGTGG TCCTCGGGATCAAGGATCTCGTCATGATTCTGAACAGGATAATTCAGACAACAATACCATCTTCGTGCAA GGCCTAGGCGAGAATGTTACAATTGAATCTGTGGCTGATTACTTCAAGCAGATTGGAATTATTAAGACAA ACAAGAAAACTGGACAGCCTATGATTAATTTGTACACAGACAGGGAAACTGGCAAGTTGAAGGGTGAGGC AACAGTTTCATTTGATGACCCACCTTCTGCTAAAGCAGCTATCGACTGGTTTGATGGTAAAGAATTCTCT GGGAATCCTATTAAAGTTTCATTTGCTACCCGCCGAGCTGACTTCAATCGGGGTGGTGGAAATGGTCGCG GAGGCCGAGGGCGAGGAGGACCCATGGGCCGTGGAGGCTATGGAGGAGGTGGCAGTGGTGGTGGTGGCCG GGGAGGATTCCCCAGTGGAGGTGGTGGAGGTGGAGGACAGCAACGAGCTGGAGACTGGAAGTGTCCTAAT CCTACATGTGAGAACATGAACTTCTCTTGGAGAAATGAATGCAACCAGTGTAAGGCACCTAAGCCAGATG GCCCAGGAGGGGGACCAGGAGGCTCTCATATGGGGGGAAACTATGGAGATGATCGACGTGGCAGAGGAGG ATATGACCGGGGCGGCTACCGGGGCCGAGGAGGGGACCGTGGGGGCTTCAGAGGGGGCCGGGGTGGTGGG GACAGAGGCGGTTTTGGCCCTGGCAAGATGGACTCCAGGGGCGAGCACAGACAGGATCGCAGGGAGAGGC CATATTAGCCTGGCTCCTGAAGTTCTGGAACTCTTCCTGTACCCAGTGTTACCCTTGTTATTTTGTAAAC TTACAATTCAGGATCGCTCATGGATATTTTTTTTTGGGGGGGGTGGGGCGGTTGTGTGTGTATGTGTGTG TGTGTGTGTCAGACTACCCTAATTGTAACCATATCTCTGGTTCCCATTAAAAAACATCATTTTAGTTAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC058247
Insert Size	1554 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC058247, AAH58247
RefSeq Size	1875 bp
RefSeq ORF	1554 bp
Locus ID	233908
Gene Summary	DNA/RNA-binding protein that plays a role in various cellular processes such as transcription regulation, RNA splicing, RNA transport, DNA repair and damage response. Binds to nascent pre-mRNAs and acts as a molecular mediator between RNA polymerase II and U1 small nuclear ribonucleoprotein thereby coupling transcription and splicing. Binds also its own pre-mRNA and autoregulates its expression; this autoregulation mechanism is mediated by non-sense-mediated decay. Plays a role in DNA repair mechanisms by promoting D-loop formation and homologous recombination during DNA double-strand break repair (By similarity). In neuronal cells, plays crucial roles in dendritic spine formation and stability, RNA transport, mRNA stability and synaptic homeostasis (PubMed:16317045, PubMed:25968143).[UniProtKB/Swiss-Prot Function]

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