Pten (BC021445) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	MMAC1, TEP1
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC021445 CCACGCGTCCGCGGACGCGTGGGCGGACGCGTGGGCCCACGCGTCCGGCCGCCGCAGCAGCCATTACCCG GCTGCGGTCCAGGGCCAAGCGGCAGCAGAGCGAGGGGCATCAGCGACCGCCAAGTCCAGAGCCATTTCCA TCCTGCAGAAGAAGCCTCGCCACCAGCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCT CCCAGACATGACAGCCATCATCAAAGAGATCGTTAGCAGAAACAAAAGGAGATATCAAGAGGATGGATTC GACTTAGACTTGACCTATATTTATCCAAATATTATTGCTATGGGATTTCCTGCAGAAAGACTTGAAGGTG TATACAGGAACAATATTGATGATGTAGTAAGGTTTTTGGATTCAAAGCATAAAAACCATTACAAGATATA CAATCTATGTGCTGAGAGACATTATGACACCGCCAAATTTAACTGCAGAGTTGCACAGTATCCTTTTGAA GACCATAACCCACCACAGCTAGAACTTATCAAACCCTTCTGTGAAGATCTTGACCAATGGCTAAGTGAAG ATGACAATCATGTTGCAGCAATTCACTGTAAAGCTGGAAAGGGACGGACTGGTGTAATGATTTGTGCATA TTTATTGCATCGGGGCAAATTTTTAAAGGCACAAGAGGCCCTAGATTTTTATGGGGAAGTAAGGACCAGA GACAAAAAGGGAGTCACAATTCCCAGTCAGAGGCGCTATGTATATTATTATAGCTACCTGCTAAAAAATC ACCTGGATTACAGACCCGTGGCACTGCTGTTTCACAAGATGATGTTTGAAACTATTCCAATGTTCAGTGG CGGAACTTGCAATCCTCAGTTTGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC ACGCGGCGGGAGGACAAGTTCATGTACTTTGAGTTCCCTCAGCCATTGCCTGTGTGTGGTGATATCAAAG TAGAGTTCTTCCACAAACAGAACAAGATGCTCAAAAAGGACAAAATGTTTCACTTTTGGGTAAATACGTT CTTCATACCAGGACCAGAGGAAACCTCAGAAAAAGTGGAAAATGGAAGTCTTTGTGATCAGGAAATCGAT AGCATTTGCAGTATAGAGCGTGCAGATAATGACAAGGAGTATCTTGTACTCACCCTAACAAAAAACGATC TTGACAAAGCAAACAAAGACAAGGCCAACCGATACTTCTCTCCAAATTTTAAGGTGAAACTATACTTTAC AAAAACAGTAGAGGAGCCATCAAATCCAGAGGCTAGCAGTTCAACTTCTGTGACTCCAGATGTTAGTGAC AATGAACCTGATCATTATAGATATTCTGACACCACTGACTCTGATCCAGAGAATGAACCTTTTGATGAAG ATCAGCATTCACAAATTACAAAAGTCTGATTTTTTTTTTTCTTATCAAGAGGGATAAAATACCATGAAAA AAAAAACTTGAATAAACTGAAATGGACCTTTAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC021445
Insert Size	1212 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC021445, AAH21445
RefSeq Size	1515 bp
RefSeq ORF	1212 bp
Locus ID	19211
Gene Summary	This gene encodes a phosphatase with dual activity against phospholipids and proteins, and acts as a tumor-suppressor. The protein contains four structural domains, a PIP2-binding domain, a catalytic tensin-type phosphatase domain, a C2 tensin-type domain and a PDZ-binding domain. The protein belongs to the protein tyrosine phosphatase family. Deletion of this gene in mice contribute to tumorigenesis in multiple tissues. [provided by RefSeq, Sep 2015]

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