Ccrk (BC031907) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	PNQLARE, MGC38901, CDCH, p42
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC031907 TGCTGGATCCCTGTAGAGTAGGATCTGGGCACCGCGGGCCGACATGGACCAGTATTGCATCCTCGGTCGC ATCGGGGAGGGCGCCCACGGCATCGTCTTCAAAGCCAAGCACGTAGAGACTGGCGAGATCGTTGCCCTCA AGAAGGTGGCCTTGCGGCGGCTGGAGGATGGTATTCCTAACCAGGCCCTCAGAGAAATCAAGGCTCTGCA GGAGATAGAGGACAGTCAATATGTGGTGCAGCTGAAGGCTGTGTTCCCACATGGTGCGGGCTTTGTGCTG GCTTTCGAATTTATGCTGTCGGACCTGGCAGAGGTGGTGCGCCATGCCCAGAGGCCCCTGGCCCCAGCAC AAGTCAAGAGCTACCTGCAGATGCTGCTCAAAGGTGTTGCGTTTTGCCATGCCAACAACATTGTGCATCG GGACCTGAAGCCTGCTAACCTGCTCATCAGTGCCTCAGGCCAGCTCAAGATAGCTGACTTTGGCCTGGCC CGGGTCTTCTCTCCGGATGGTGGTCGCCTCTACACACATCAGGTGGCCACCAGGTGGTACCGAGCTCCTG AACTCCTGTATGGCGCTCGGCAGTATGACCAGGGCGTTGACCTATGGGCTGTGGGCTGCATCATGGGAGA GCTGTTGAATGGGTCCCCCCTGTTCCCGGGCGAAAACGACATTGAACAACTGTGCTGTGTGCTTCGCATC CTGGGTACCCCGAGTCCTCGAGTCTGGCCGGAGATCACAGAGCTGCCTGACTACAACAAGATCTCCTTCA AGGAGCAGGCACCAGTGCCCCTGGAGGAGGTGCTGCCTGATGCCTCTCCCCAGGCCTTGGACCTGCTGGG CCAGTTCCTCCTCTACCCTCCACGACAGCGTATTGCAGCCTCCCAGGCCCTTCTGCATCAGTACTTCTTC ACAGCGCCTCTGCCTGCCCATCCATCCGAGCTGCCAATTCCTCAGCGCCCAGGGGGACCTGCACCCAAGG CTCACCCAGGGCCCCCCCATGTCCACGACTTCCATGTGGATCGACCTCTTGAGGAGTCACTGTTGAACCC AGAACTGATTCGGCCCTTCATCCCAGAGGGGTGAGATGCTGGTCCAGGCCTTCCTGCTCGCCCTAGGAGC ACCTCTTTCTGATTTGCCTCCATGGCCTCCCCACGGCTATATATACCACACCTGGTCCTGCTCCTTAGTG TGCTTGAGGGCTGGGCTCTGGGAGGCAGAACCGTGAGATGTTCATCCCAGCAGAGAAAGAGACTCACGTC CTACAGACAAAGCCTCCAGAAACTGCTAGCTGTGTCCTTCTCCAGGGCCACCCCTCAGTGGTGCCACCCG GCCTTAGAGATGATTGTCAGGCTCTGTCCCCTCTTCAAGGACATTGGTACTACAGCACCACCTGGTGGAA GCACAGAGTATAAGCTGTCTTCATACCGGGGACACAGCTGGGAAGTCAGACATGTTTTAGTTTTGGTTCC ACTGGGTCAGGATTTGAGGTTCATATAAAAGCCCTGGGTGTTTCTGTCTAATTGCACCTTGTCTGTTGCT GTTAGGGAAAGGACAATGGTGGGCCTTGATTCACAGGGGTCAGGTACTCAGAAGAGGCCTCCTGTGAAGG CCATTTGGGTCCTCAGGCTTCCCATGCTATTCACGGGACTTGAGTGCTCATTTGGGAGCGAGAGTCCAGA AGCTGAGGCCCAGGGATGGACAGTCCAGTTCCCGAAGCACACTTCCCACATGTCGGGTGGGTCAGTCAGT GAGCCTGAGGCTGCCTTGCAGATGCGGAAGCAGACATTCCTGGAATCCACTCAGTAAATAAATTCCAGTG TGACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC031907
Insert Size	1041 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC031907, AAH31907
RefSeq Size	1854 bp
RefSeq ORF	1041 bp
Locus ID	105278
Gene Summary	Involved in cell growth. Activates CDK2, a kinase involved in the control of the cell cycle, by phosphorylating residue 'Thr-160' (By similarity). Required for high-level Shh responses in the developing neural tube. Together with TBC1D32, controls the structure of the primary cilium by coordinating assembly of the ciliary membrane and axoneme, allowing GLI2 to be properly activated in response to SHH signaling.[UniProtKB/Swiss-Prot Function]

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