Cdkal1 (BC016073) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	RP23-202F3.2
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC016073 GGTTTTTTTCCTCTCAGTTCGGACAGATTCATCTTTCAAGAGGACTTGAGACCAATTTCAGAAAATCAAG ATGCCTTCTGCGTCTTGTGATGTACTGCTGGATGACATAGAAGATATCATATCACAGGAAGATTCAAAGC CACAGGACAGACAGTTCTCAAGAAAGCATGTGTTTCCTAAAGTACGAAGGCGGAATACCCAAAAATATTT GCAAGAAGAGCCCAGGCCACCAAGTGACAGCACTATTCCAGGCATACAGAAAATTTGGATCCGAACATGG GGCTGCTCACATAATAATTCGGATGGAGAATACATGGCTGGACAGCTTGCTGCCTATGGCTATAAAATTA CAGAGAATGCATCAGATGCAGATCTGTGGCTCCTGAACAGTTGTACTGTGAAAAATCCAGCTGAAGACCA CTTTAGAAACTCAATCAAAAAAGCTCAAGAAGAGAATAAGAAAGTTGTCCTCGCTGGCTGTGTTCCCCAA GCCCAGCCTCGTCAGGACTACCTCAAAGGACTGAGCATCATCGGGGTTCAGCAGATAGATCGTGTGGTAG AAGTTGTGGAGGAAACAATAAAAGGTCACTCAGTGAGGCTCCTGGGCCAGAAGAAAGACAACGGGAAGCG CTTGGGTGGAGCTCGACTGGATTTGCCGAAGATCAGAAAGAATCCGCTGATAGAAATCATTTCCATCAAC ACGGGGTGTCTCAATGCTTGTACCTACTGCAAAACTAAACACGCCAGAGGAAACCTGGCCAGCTATCCCA TTGATGAGCTTGTGGAGAGAGCCAAGCAATCCTTTCAAGAGGGTGTTTGTGAGATATGGCTGACCAGCGA GGACACCGGGGCCTATGGCAGAGACATTGGCACAGATCTCCCCACACTCCTGTGGAAGTTGGTGGAAGTG ATTCCTGAGGGAGCCATGCTGAGGCTGGGCATGACAAATCCACCATATATTTTAGAACATCTGGAGGAAA TGGCAAAAATCCTCAACCATCCCAGAGTCTACGCTTTCCTCCACATCCCAGTGCAGTCCGCCTCTGACAG TGTGCTCATGGACATGAAGAGAGAGTACTGCGTGGCCGACTTCAAAAGAGTCGTGGATTTTCTCAAGGAG AAGGTTCCTGGAATAACGATTGCTACGGATATTATCTGTGGTTTTCCTGGAGAAACCGATCAGGATTTTC AAGAAACAGTGAAACTTGTTGAAGAGTACAAATTTCCAAGCCTCTTCATCAACCAGTTCTATCCAAGACC CGGAACTCCTGCTGCAAAAGCCGAGCAGGTTCCAGCACATGTGAAAAAACAAAGGACAAAAGATCTTTCT CGGGTGTTTCATTCCTACAATCCATACGATCACAAGATTGGCGAAAGACAACAAGTGCTAGTCACTGAAG AGTCTTTTGACTCCAAGTTTTATGTTGCTCATAACCGATTCTATGAGCAGGTTTTAGTACCAAAGAACCC TGCATTCATGGGGAAAATGGTTGAAGTCGACATTTACGAATCAGGAAAGCACTTTTTGAAAGGACAGCCA GTATCAGAAACCAGAGTGTATACACCATCCATCAGCAAGCCACTGGCCAAGGGAGAAGTCTCAGGTTTGA CAAAGGAATTCAGAAATAGGCTTGGGAATCATCCAAACGGAACTTCAGACACCTGTCCTGCAACTCAGCA TGGCTCTGCGTATTCCAGAATGGTGCTGCAGATGTCCCAGTATGACTGCGCACTGAAGGTGGCTACAGGC CTGGCTCTCCTCGCTCTCCTTTTGCATTTCTGGCCAGACTCTTTGCTTACAATGTAACTAAGTAAAGCCT CCATAAGGAATAAGTTTCTAATTCAAGTGGTCAACAAACAAGAGGACATCAACACCAGTTTTCAAGAGAC TGCCTTCTGAACTAGACACACTGCCTTGTCTGGATCTCTTTTGAATGCCCTCACACTGAGCTGAGCCATC TGACCTCAGACCAGATCACAGTTTCTGAACATGTCTTTCTCATCAAACTCCTTCAGACTTCTTCCTAACA ACAAATACAAGTGGTTGGGTTTTTTGGTTGGTTTTTGGTTTTTTTTGTTTTGTTTTGTTTTTTTTTTGTT TTTTGTTTTGTTTTTTTTTTTTTTTGTCTCCAGTCATTGAATCCCAGCCTAAGTGCACAGAGGCTCTGCC TATTCGTGGCTCTGGCCATCCTTTCCTTGTGTGGAAGAACATCCAGCCCTTAATAAACATTCTCCCGGCT GTGTTTGGAGTCCCCGTGCTACAAACATCTTTTTCACAGCAGATGTGTTCCTAAAAAGTTTATATAGAAA CCTATTTTGAGAACTTGGACCCAAGTCCACCACTTAAAGGACTCTTCCTGGCGTTCTAGAAAATGTGAAG ATTCCATGCCTGTGGCACCCACAAACTTTCAAACAAACCATGATCCTTTCCTGACACACCGACCAGGCAT GTTACATTTATCATTTTGACCCAACACCACCAGCCGTCTTTACTAAATGAAATGACTTCTTTCACAATAA ACAGAATTGGTACAATAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC016073
Insert Size	1737 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC016073, AAH16073
RefSeq Size	2551 bp
RefSeq ORF	1737 bp
Locus ID	68916
Gene Summary	Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.[UniProtKB/Swiss-Prot Function]

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