Bag5 (BC037642) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1600025G07Rik; 4930405J06Rik; Bag-5
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC037642 CCACGCGTCCGGGTTGGCGTTACGGCGGCGTCGCAAAGCCCGAGCCGACGCGCGGCGAGGCCGACCGTGC GCATTCCCTGGGACGGACGCCGGGCGGCGGGGCCCGCGCGGGACGAGGAGAGGACAAGTTTTCCCCGGGC TCACTGCTGACGGGCCCGCTCTGCGAGGACGGCGACAACGCTGCGGAAGTGTAGGTGGCGGGGCGCGATG GCGCGTGCGGCCCAGTGCTTGTGAAACTGAACACAGAAGTATGGATATGGGAAACCAACACCCCTCCATT AGTAGGCTTCAGGAGATCCAGCGGGAAGTAAAGGCTATAGAGCCGCAAGTGGTCGGCTTCAGCGGCCTGT CGGATGACAAGAACTACAAGAGGCTGGAGAGGATTCTGACCAAACAACTTTTTGAAATTGACTCTGTGGA TACGGAAGGAAAAGGAGATATCCAGCAAGCTAGGAAGCGGGCGGCACAGGAGACAGAACGTCTCCTCAAG GAGTTGGAGCAGAATGCCAACCACCCACACCGCATCGAAATCCAGAATATCTTTAAGGAGGCTCAGGCCC TTGTGAAAGACAAGATTGTGCCCTTTTACAGCGGAGGCAACTGTGTGACTGACGAGTTTGAGGAAGGCAT CCAGGACATCATTCTGAGGCTGACGCACGTGAAGACCGGAGGGAAGGTCTCCTTGCGGAAAGCCAGGTAC CGCACGCTCACCAAGATCTGCGCGGTACAGGAGGTTATTGAGGACTGTATGAAGAAGCAGCCGTCCCTGC CACTGTCTGAGGATGTGCATCCTTCAGTGGCCAAAATCAACTCTGTCATGTGCGAGGTGAACAAGGCCAG AGGGACGCTGATCGCACTTCTGATGGGCGTGGACAGTTCCGAGACTTGCAGGCACTTATCCTGCGTGCTG TCAGGACTGATTGCTGACTTAGATGCTCTAGATGTGTGTGGGCGTACAGAGATCAGAAATTATCGGAGGG AGGTGGTGGAAGATATCAACAAATTACTGAAATACCTAGACTTAGAAGAGGAAGCTGACAGTACACATGC ATTTGACCTGGGACAGAACCATTCCATTATAAAAATAGAAAATGTTCTCAAGAGGATGAGAGAGATAAAA AATGAACTTCTCCAGGCGCAGAGCCCTCCTGAGTTGTACCTGAGGGCCAAGACAGAGCTGCAGGGCTTGA TCGGACAGCTGGATGAAGTGAGTCTGGAGAAAAACCCCTGCATCCGGGAGGCCAGGAGGAGGGCGGTGAT TGAGGTGCAGATCCTGATCACGTACCTGGACCTGAAGGAAGCCCTCGAGAAGAGGAAGCTGTTCCCATGT GAGGAGCACCCCCCACACAAGGCCGTGTGGGAGATCCTTGGGAACCTGTCTGAGATCCTGGGCGAAGTCC TTTCATTTGGTGGAAACCGAACTGATAAGAACTACATCCGTCTGGAGGAGCTACTGACCAAACAGTTGCT GGCTCTGGATGCCGTTGACCCACAGGGAGAAGAGAAGTGTAAGGCTGCCAGGAAACAGGCTGTGAAACTG GCCCAGAACATCCTCAGCTACCTGGACATGAAGTCGGACGAGTGGGAGTATTGATGGGCCAGGAGCTGAT CTCGCTGGTTCGCACCTCATCTCCTTTAGAGAGCTCTCAGCTCATGGAGCCTAAATGTAATTTCTATGTG CATAGTCAATCTCAGTATTTATGATTTAATCAAATTATATTCAGTATTTCTGCTGCTTTTGATGTTGCAA ACGAATATCATTATAGCACATTACCTTTTCAAAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC037642
Insert Size	1344 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC037642, AAH37642
RefSeq Size	1800 bp
RefSeq ORF	1344 bp
Locus ID	70369
Gene Summary	May function as a nucleotide exchange factor for HSP/HSP70, promoting ADP release, and activating Hsp70-mediated refolding. Inhibits both auto-ubiquitination of PRKN and ubiquitination of target proteins by PRKN (By similarity).[UniProtKB/Swiss-Prot Function]

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