Vps36 (BC010811) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1700010A24Rik; 2210415M20Rik; 2810408E15Rik; Eap45
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC010811 GGGTGGCGGCCATGGACCGCTTCGTGTGGACCAGCGGCCTCCTGGAGATCAACGAGACCCTGGTTATCCA GCAGCGCGGGGTGCGCGTCTACGACGGCGAGGAGAAGATAAAATTTGATGCCGGGACTCTTCTTCTTAGT ACACACCGGCTGATTTGGAGAGACCAGAAGAATAATGAGTGCTGCATGGCCATTCCCCTGTCTCAGATTG TGTTCATCGAGGAGCAGGCAGCTGGAATCGGGAAGAGCGCCAAAATCGTGGTTCACCTGCACCCAGCTCC TTCTAACAAAGAGCCCGGTCCGTTCCAGAGCAGTAAGAACTCCTACATCAGACTCTCCTTCAAAGAACAT GGCCAGATTGAGTTTTACAGGCGTTTATCAGAAGAAATGACACAGAGAAGATGGGAGACTGTGCCAGTTT CCCAGTCACTACAAACAAATAAAGGACCCCAGCCAGGAAGAGTAAGAGCTGTTGGAATTGTAGGTATTGA AAGGAAACTTGAAGAAAAAAGAAAAGAAACCGATAAAAACATTTCTGAGGCCTTTGAAGACCTGAGCAAG CTGATGATTAAGGCTAAGGAAATGGTGGAGTTATCCAAATCAATCGCTAATAAGATTAAAGAGAAGCAAG GCGATGTCACAGAGGATGAGACCATCAGGTTTAAGTCGTACCTGCTGAGTATGGGAATAGCCAACCCAGT CACCAGAGAAACCTATGGCTCCGGCACACAGTACCACATGCAGCTGGCCAAACAGCTGGCTGGAATATTG CAGGCACCTTTGGAGGAGCGTGGGGGAATAATGTCCCTCACAGAGGTGTACTGTCTAGTGAACCGGGCCC GAGGAATGGAGTTGCTCTCGCCAGAAGATTTGGTAAATGCGTGCAAGATGCTGGAAGCGCTAAAATTGCC TATCAGGCTTCGGGTTTTTGACAGTGGTGTCATGGTAATTGAGCTTCAAACTCACAAGGAAGAGGAAATG GTGGCCTCGGCCCTGGAGACGGTTTCAGAAAGGGGGTCCTTAACATCCGAAGAGTTTGCCAAGCTTGTGG GAATGTCTGTCCTTCTGGCTAAAGAAAGGCTGCTCCTTGCAGAGAAGATGGGCCACCTTTGCCGAGACGA CTCCGTGGAAGGCTTGCGGTTTTATCCAAATTTATTTATGACACAGAACTAAGAATTTTGTACCTGAAAT GCTTTATGTCAATAAGCTTGCATTTTGCCAAGGTCTCACGACACTGAGCTAAGAGATAAACCCAAATAAA CCAAGAATGTGTTAGAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC010811
Insert Size	1161 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC010811, AAH10811
RefSeq Size	1290 bp
RefSeq ORF	1161 bp
Locus ID	70160
Gene Summary	Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Its ability to bind ubiquitin probably plays a role in endosomal sorting of ubiquitinated cargo proteins by ESCRT complexes. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. Binds phosphoinosides such as PtdIns(3,4,5)P3.[UniProtKB/Swiss-Prot Function]

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