Nkd2 (BC019952) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2210403L10Rik; AW212591
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC019952 GCCGCTGCCCGCGCCGGTGTCTCCGGTTGCCGGGCCAAGCCTCGCCAGACTGCCCGGCCCCGGATCCAGG CTCCACGAGTTGCTCTCAGCTCGGGATTCACGCACCCCGGGCTCGAACCTCGGACGCACACCTGAGAGCT CGGCCGGAGGGTGGGTCCAGGCCCAGCGGAGACATGGGGAAATTTCAGTCCAAGCACGCCGCAGCAGCCT GCAAGAGGAGAGAGAGTCCCGAAGGGGACAGCTTCGTGGTGCCTGCGTACGGGAGCGGTCGCAGAGGAGC GGAAGAAACGGACCGGCGTGCGGGCAGCGGTGTGGAACATCGCTCACGGGACAAGCAGGAGCTGCTCAAT GGAGACCCTAAGGAGGGGCCTTTCTGGGACGACAAGGGTTCCCTAGAAGTTGCGCTGCCCCCTGAGAAGT CTGAGGGCCATGAGGGCCAGGGCCAGCTCTTCAGCACAGATGATGGGGAGAAGGCAGCAAGCCGTGAGGG TCCACTGAGACTTAGCAAGAAGCACTTGAACATTGACGCACTGCAGTGTGACGTCTCAGTGGAAGAAGAC AACCGCCAAGAATGGACATTCACGTTGTATGACTTTGACAACAGTGGGAAAGTCACCAGAGAGGATATGT CCAGCCTGATGCACACCATCTACGAGGTTGTCGATGCCTCTGTCAATCACTCCTCCGGCAGCAGCAAGAC CCTTCGAGTGAAGCTAACTGTCAGCCCTGAACCCTCCAGCAAGAAGGAATGTCCTCTCACTGGCCAAGAC CGGGAGCCCACTCGTGGCAGAACAGAGATTGAGCTCACAGATGAGCCCCGAGCGGCTGACAGAAGGCTAT CCGCCTACAGCAGGAAGCCCAATGCTGATCCCCAGCCCTGCTCTGTGCGAGTGCCCTACTGTGTGGATGA GAACACAGAGCGCAGAAACCACTACCTAGACCTTGCTGGCATCGAGAACTACACATCTAAGTTTGGTCCT GGGTCACCACCTGAGCAGGCCAGGCAAGAACATCATGGCAGGGCCACACACATTCCAAGCAGGTCCCGAT CACAAGAGTCGGATGCCCACGCTATACACCACCGCAGGTCTCAAGTCCTGGCTGAGCATGTCATACCAGC TAATGAGCCTGCCACCCGGGCCCTGGCTGCACAGCCCCGGCTCAAGGGGCAGGAGAAGCAGTTCCTCAGG TCTCCTAAGGGTCCAGGAAAACCTCTTGGGACACCAGGCAGTGGCAAGCCAGGGAAAGCTCTCAGCTATT GCCTGCAGGCTGTGCCACTGCCCCAGGGTGCTCAGGATGGCCACCACCTTCCTCAGCCCCCACCACAGCC TCCACCGCAGCCCTATGGTCACAAGCGGTACCGGCAGAAAGCCAGAGAAGGCCACTCACCACTTAAGGGG CATGGCCAGCCTACCATGGTGGAGCATGAAGTGGTACGGGACCTGCCTCCCATGCTGGGGCCTGAGGGCT ATGTGATGCCTGTGGTCCAGAGGCATGAACACCACCATCACCATGAGCACCACCACCATCACCACCACCA CCACTTCCACCCATCCTAGCACCCAAACAAGCACTCTGCCCAAATGCCCTTGGGAACAGAGCAGGGGCTG GCATGTGCCTAGTAGAGAATACAGCATCCCCTGGCTCAACACCAGCTACAGATGTCAAGGGGGCACACCA GGGAGATGGCACCAGGCTTGGATATTCATGGACAAAGCTACCTTTCTCCATGCCATTTGGATACTAGTAC ATTTGATGCAGCTGATGGTTACCAGGGACACGTGCTCCTGTGTCATCCAGAAAGCCCATTCATGAGGGCA CAAAAGCATCTGTTCCTGCTTTTGCTGCAGTGTGGCCATGGGATATTTGAAGGCATTCTTTGTATCCTCA TGGTTCTTTGACCTTCGGGTACCTGAATAGAAAAGGCAATGCTTTTTGTGAAAAAAAAAAAAAAAAAAAA AAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC019952
Insert Size	1386 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC019952, AAH19952
RefSeq Size	1967 bp
RefSeq ORF	1386 bp
Locus ID	72293
Gene Summary	Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity. Required for processing of TGFA and for targeting of TGFA to the basolateral membrane of polarized epithelial cells (By similarity).[UniProtKB/Swiss-Prot Function]

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