Smpd2 (BC010978) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	nSMase1, nSMase
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC010978 CCACGCGTCCGGTGGAAGCCGAGCCGGGAACAAGGGAGGAACCTGTAGGCCGCGGTGCGGAGAACCCACC GAAGGACCTAAGAATCTGGAACAGTCCACCCGAGATTCCTTCCAGGACTGCCGGCGGCCTCGCGCAGCCA GCCCGGGATTTGCAGCCGACCTTCTTTCCGGGTGGAAGGACGGCCTTTGTCCCAGTAACGCAGGAGTCGC CCCCCACCCCCAACCAGCTCGCGTTCCTGGGTCGGGGCAGCGCAGGACAGGGCAATAAGCCTGTGCGCGC AATCCGCCTCGCCGCCCTTGCTCCGAAGCACTCCAGCCATGAAGCTCAACTTTTCTCTACGGCTGAGAGT TTTCAATCTCAACTGCTGGGACATCCCCTACCTGAGCAAACATAGGGCGGACCGCATGAAGCGCTTGGGA GACTTTCTGAACTTGGAAAACTTTGATCTGGCTCTCCTGGAGGAGGTGTGGAGTGAGCAGGACTTCCAGT ACCTAAGGCAAAGGCTATCGCTCACCTATCCAGATGCACACTACTTCAGAAGCGGGATGATAGGCAGTGG CCTCTGTGTGTTCTCCAAACACCCAATCCAGGAAATCTTCCAGCATGTCTACAGTCTGAATGGTTACCCC TACATGTTCCATCATGGAGACTGGTTCTGTGGGAAGTCTGTGGGGCTGCTGGTGCTCCGTCTAAGTGGAC TGGTGCTCAATGCCTACGTGACTCATCTACATGCTGAGTACAGCCGACAGAAGGACATCTACTTTGCACA CCGTGTGGCCCAAGCTTGGGAACTGGCCCAGTTCATCCACCACACATCCAAGAATGCAGATGTGGTTCTA TTGTGTGGAGACCTCAATATGCACCCCAAAGACCTGGGCTGCTGCCTGCTGAAAGAGTGGACAGGGCTCC ATGATGCTTTCGTTGAGACTGAGGACTTTAAGGGCTCTGATGATGGCTGTACCATGGTACCCAAGAACTG CTACGTCAGCCAGCAGGACCTGGGACCGTTTCCGTCTGGTATCCGGATTGATTACGTGCTTTACAAGGCA GTCTCTGAGTTCCACGTCTGCTGTGAGACTCTGAAAACCACTACAGGCTGTGACCCTCACAGTGACAAGC CCTTCTCTGATCACGAGGCCCTCATGGCTACTTTGTATGTGAAGCACAGCCCCCCTCAGGAAGACCCCTG TACTGCCTGTGGCCCACTGGAAAGGTCCGATTTGATCAGCGTGCTAAGGGAGGCCAGGACAGAGCTGGGG CTAGGCATAGCTAAAGCTCGCTGGTGGGCTGCATTCTCTGGCTATGTGATCGTTTGGGGGCTGTCCCTTC TGGTGTTGCTGTGTGTCCTGGCTGCAGGAGAAGAGGCCAGGGAAGTGGCCATCATCCTCTGCATACCCAG TGTGGGTCTGGTGCTGGTAGCAGGTGCAGTCTACCTCTTCCACAAGCAGGAGGCCAAGGGCTTATGTCGG GCCCAGGCTGAGATGCTGCACGTTCTGACAAGGGAAACGGAGACCCAGGACCGAGGCTCAGAGCCTCACC TAGCCTACTGCTTGCAGCAGGAGGGGGACAGAGCTTAAGAGCTTAACAATAAAACTTGCTTGACACACTC TAAAAAAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC010978
Insert Size	1260 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC010978, AAH10978
RefSeq Size	1631 bp
RefSeq ORF	1260 bp
Locus ID	20598
Gene Summary	This gene encodes a protein with similarity to the human nSMase1 protein. In humans, the nSMase1 protein was initially identified as a sphingomyelinase based on sequence similarity between bacterial sphingomyelinases and a yeast protein. Subsequent studies showed that its biological function is less likely to be as a sphingomyelinase and instead as a lysophospholipase. [provided by RefSeq, Oct 2009]

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