Wnt6 (BC048700) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AA409270; Wnt-6
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC048700 CCGCCGCGCCCTCCTCGCCCGGGATGGGCCCCCCCGCCGCCACCGCCGCCGGAGCCCTAGTCTCCGGGCC GCCGCCTCGGTCGCCGCGTTTGCCCTGAAGCCCGGTGCCCGCGCGCCCCGGCTCACCCCGCAGCTTCACT CCCCACCCCCAGCCGCCTCCCCGGCCAGACTGCGGTAGAGCTCTCAGGATGCTGCCGCCGGTGCCCTCCC GCCTCGGACTGCTGCTGCTGCTCTTGTGCCCCGCGCACGTCGATGGACTGTGGTGGGCCGTGGGCAGCCC CTTGGTCATGGATCCTACCAGCATCTGCAGGAAGGCCAGGCGGCTGGCAGGAAGACAGGCCGAGCTGTGC CAGGCGGAGCCGGAAGTAGTGGCAGAGCTTGCCCGAGGCGCAAGACTGGGGGTTCGAGAATGTCAGTTCC AGTTCCGTTTCCGACGCTGGAACTGCTCCAGCCACAGCAAGGCCTTTGGGCGCGTCCTGCAGCAGGACAT CCGAGAGACAGCTTTCGTGTTTGCAATCACCGCAGCTGGTGCCAGCCACGCGGTCACTCAAGCCTGTTCC ATGGGAGAGCTCCTACAGTGTGGTTGTCAGGCACCCCGCGGGCGGGCACCGCCTAGGCCCTCCGGCCTTC TGGGCACTCCTGGACCTCCAGGACCAACTGGCTCTCCAGATGCTAGCGCAGCCTGGGAGTGGGGAGGCTG CGGAGACGATGTGGACTTCGGGGATGAGAAGTCAAGACTCTTTATGGATGCGCAGCACAAGCGGGGCCGT GGAGATATCCGTGCATTGGTGCAACTGCACAACAACGAGGCGGGCAGGCTGGCGGTGCGGAGTCACACGC GCACCGAGTGTAAGTGCCATGGGCTTTCGGGTTCCTGCGCTCTGCGCACCTGCTGGCAGAAGCTGCCTCC GTTCCGCGAGGTGGGCGCACGGCTGCTGGAGCGCTTCCACGGCGCCTCGCGCGTCATGGGCACCAACGAC GGCAAAGCTCTGCTGCCTGCGGTCCGCACACTCAAGCCTCCCGGACGAGCGGATCTCCTCTACGCAGCCG ATTCACCCGACTTCTGCGCCCCCAACCGGCGCACGGGTTCGCCGGGCACGCGCGGACGCGCCTGCAACAG CAGTGCCCCGGACCTCAGCGGCTGCGACCTGTTGTGCTGCGGTCGCGGGCACCGCCAGGAGAGCGTACAG CTCGAGGAGAACTGTCTGTGCCGCTTCCACTGGTGCTGCGTGGTGCAATGCCACCGCTGCCGGGTGCGCA AGGAACTCAGCCTGTGCCTCTGACCCGTCGCCTGCCTCGGAACTGCTGGCAGCCACCTCTGGGCCATCTA CAGGACTATTAGATTCCAGCAGGGGGCGCTGTCTGAGTCCAGCAGCTCCCTAGGAAAAGTACCTATCCAG GCCTTGGGAAATTACAGGGGCCAGCCAGGAACTTGGGGTTTACACCAGCCCACGAAAGCCCGGGGGAACA TACCCCTCCAGCATTCCCCTGAAAGGCCCTTTGCTAGTTCCTGCAGGAGATCACTCCCCTTGGCCCCCCA GATGGAAATAAGAAAGCCAGACTCTGCCCTCTGGAAATAATATTCCTCAGAATTACTGGGATGGATGGGT GAGTTTAGTATCAATAAAGACATTTAAATCCACAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC048700
Insert Size	1095 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC048700, AAH48700
RefSeq Size	1669 bp
RefSeq ORF	1095 bp
Locus ID	22420
Gene Summary	Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.[UniProtKB/Swiss-Prot Function]

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