Ppp1r1a (BC025123) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	0610038N18Rik; I-1
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC025123 CGGGCTGGGGCCAGGGCCGGAGCGCAACAGAGAGGGAGCGCGCCCGCCCCAGCCCGAGGTCCGCCGCTGC CGGACCGGGAGCCATGGAGCCCGACAACAGCCCACGGAAGATCCAGTTTACGGTCCCGCTGCTGGAGCCT CACCTGGACCCGGAGGCGGCGGAGCAGATTCGGAGGCGCCGCCCCACCCCTGCCACACTTGTGCTGACCA GTGATCAGTCCTCCCCAGAAATAGATGAAGACCGGATCCCCAACTCACTTCTCAAGTCCACCTTGTCAAT GTCTCCACGGCAACGGAAGAAGATGACAAGGACCACACCCACCATGAAAGAGCTCCAGACGATGGTTGAA CATCACCTAGGGCAACAGAAGCAAGGGGAAGAACCTGAGGGAGCCACTGAGAGCACAGGGAACCAGGAGT CCTGCCCACCTGGGATCCCAGACACAGGCTCGGCGTCAAGGCCAGATACCCCCGGGACAGCACAAAAGTC TGCAGAATCCAACCCCAAGACTCAGGAGCAGTGTGGCGTGGAGCCCAGAACAGAGGATTCTTCAGCCCAC ATGCTACCACTGGATTCCCAGGGAGCCAGCTTGGTCTGACAGAAGTTGGTATCCGGGGATCACCACTGCA GTGTGGAAATTCATGGACACTGGATGTTTCTTAATCTCTTGTTTTTAAAAAGTGATAAATTTGGTGTTAG GTCCTTGGAGCTTTTCTTCTTTCCAGCCCGGGGATCCCTTCCTCCTTCAGCTCTTGTCTGCACCCCACTG CCCCCACCCTCAGGACCGATTAAGGAAGGAAATAGCAAACTGACAAAAGCCTAGCCTCCACTAGCAAAAT AGAAAAATAGACGTCTGGAAGTAGGAGGTCGCCTCAAGGTTTTCCTTTGTTACTGTGGAAAGCTCTGCCC ACAGAGCAGGGTAGCCTACTTGCTGCTCTAAAGCGGGTACCCAGACCAGTGAGGGTTTGCCAAAGCGCCA TCTGCTGGACATAAAGGGGAAGTGCATCTTGTCCCTAGCTCCCTGAGAAACCTCCAATTCTGTGGCCACT GTTCCTTGTACAAACCTGACACAAAGACTGTCAGAAAACACTGTGGCTAGAAACCCAAAGGGCCACCTCT CCCATTGTCCTACATCCTAAGGTCAGAGCAAGACTAACACCATGCACAAATAGAGAAATGGTCTCTGATC CTAGCCAGGCTTCTGCTGCTGATTCTGATTTTCCCAAAGAATTAAAGCGATGAAGGGAGAAGCCAAAAAA AAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC025123
Insert Size	516 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC025123, AAH25123
RefSeq Size	1274 bp
RefSeq ORF	516 bp
Locus ID	58200
Gene Summary	Inhibitor of protein-phosphatase 1. This protein may be important in hormonal control of glycogen metabolism. Hormones that elevate intracellular cAMP increase I-1 activity in many tissues. I-1 activation may impose cAMP control over proteins that are not directly phosphorylated by PKA. Following a rise in intracellular calcium, I-1 is inactivated by calcineurin (or PP2B). Does not inhibit type-2 phosphatases.[UniProtKB/Swiss-Prot Function]

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