Kif22 (BC003427) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AU021460; C81217; Kid; Kif22a
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC003427 GGCCGAAGAAGGGAAGGCTATGAGCTTAAGGGCTAAGACGTGTCCGCAGCGACGGGAGATGGCGTCGGCC ACCTCAGGACCTGGCCGCTGTGTAAGCAAAGGAGGGCTTGGCCGGCGTCCGCCTCTAGCTCGAGTAAGGG TGGCTGTGCGGCTGCGCCCGTTTATGGATGGAGAGACAGAAGCGAAGGAGCTCCCCTGTGTCCGAGCCAT AGACAGCTGCTCTCTTGAAGTGGCTAACTGGAAGAAATACCAGGAGACGCTCAAATATCAGTTTGATGCC TTCTATGGCGAGAAGAGCACTCAGCAGGAAGTCTATGTAGGTTCAGTACAGCCCATCCTAAGGCACTTGC TGGAAGGGCAGAATGCCAGTGTACTTGCCTATGGGCCTACTGGGGCAGGGAAGACACACACAATGCTGGG CAGCCCAGAGCAACCTGGAGTGATTCCTCGGGCTCTCATGGACCTCCTGCAACTAGCAAGGGAAGAGAGT GCTGAGGGACGGCCATGGGACGTTTCTGTTGCTATGTCCTATTTAGAGATCTACCAGGAAAAGGTATTAG ACCTCTTGGATCCTGCATCAGGAGACCTCGTGATCCGCGAAGACTGCCGAGGGAACATCCTGATCCCAGG CCTCACACAGAAGCCCATCACCAGCTTCTCTGACTTCGAGCAGCACTTCCTTCCAGCCAGTAGAAATCGA GCTGTAGGAGCCACCCGGCTTAACCAGCGCTCTTCCCGTAGTCACGCAGTGCTCTTGGTCAAGGTAGATC AGCGTGAACGTTTGACTCCATTTCGCCAGCGGGAAGGAAAACTCTACCTTATTGATTTGGCTGGTTCAGA GGACAACCGTCGCACAGGCAACCAGGGCATTCGGCTCAAAGAGAGCGGAGCCATCAACACCTCCCTCTTT GTACTGGGCAAAGTGGTGGATGCATTAAACCAGGGCCTCCCTCGCATACCATACCGGGACAGCAAGCTCA CTCGTCTGCTGCAGGACTCTCTGGGAGGCTCAGCTCATAGCATCCTCATTGCCAACATTGCTCCTGAAAG ACGTTTTTACCAGGATACAATTTCAGCATTGAACTTCACTGCTAGGTCCAAGGAGGTGATTAACCGGCCT TTCACCAATGAGAGTCTACAGCCTCATGCCTTGGCACCTGTTAAGCTGTCTCAGAAAGAACTGCTAGGCC CGTCAGAGGCAAAGAAGGCCAAAGGCCCTGAAGAAGAATCAACTGGGAGCCCTGAGTCTACAGCAGCTCC AGCGTCTGCCTCCCAGAAACTAAGCCTCTTACAGAAGCTAAGCAATATGGATCCAGCCATGTTGGAGAAC CTCCTGAGTATGGAACGTTTGCTGGGTTCCCAGGGAAGCCAAGGGACCCCCCTACTGAATACCCCAAAGC GAGAACGAATGGTGCTCATGAAGACAGTGGAGGAAAAGAACTTAGAGATTGAGAGGCTTAAGATGAAGCA AAAAGAACTGGAAGCCAAGGTGTTGGCCCAGGAGGCACCGGATCCAAGGGAAAAAGAGAACACTCCCACA ATTCTCCAGCCGCCTGCCTCTTATAGTGGCACAGTGGCAAAGCCCCTGAAAAAGGCTGTAGTGATGCCCC TGCAACGGATTCAGAAGCAGCGAGAGTCCTCAAACCAAATCCAGCTCCTGAAGAAAGGCCCCAAAAGGAA GCTGGAGCCATCCCCTGAGTCAGAAGCTGTGGAAAAGGATGAGGACTACTGGGAGGTACAGATTAGCCCA GAGTTACTGGCACATGGGCGCAAAAAATTATTGGATCTGCTGAATGAAGGCTCAGCTCGGGAACTGCGCA GCCTGCAGCGCATTGGCCAGAAGAAAGCCCAGCTCATCGTGGGCTGGAGGGAGCTCCACGGCCCCTTCAG TGAGGTGGAGGACTTGGAACAGGTGGAAGGCATCTCTGGGAAGCAGGTGGAGTCTTTCCTGAAGGCGAAT CTCTTGAGCTTGGCCGCCAGCCAGCACAGCGGTCCCTCTTGACACTACCTTCTTTTAAAAATCCTTGTAT AGTGACGTGTTGTGTAAATACAGTTTTTATTCCAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC003427
Insert Size	1983 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC003427, AAH03427
RefSeq Size	2086 bp
RefSeq ORF	1983 bp
Locus ID	110033
Gene Summary	Kinesin family member that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA. Plays a role in congression of laterally attached chromosomes in NDC80-depleted cells.[UniProtKB/Swiss-Prot Function]

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