Tob1 (BC082588) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Tob; Trob
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC082588 CTGGCCCGGCGCGCAGAGGGCGCTGTACGGGGACGCAGGGCCGGGCCGCCGGGCTGTGTAGAGAAGCGAG CGCGCTCGTGTAAGCTTGCCGTCCCCGCCACCCCTGAGCCAGAGCAGCGAGCTCCCGGACCCGCCCGAGG CGTCCGTCGCGTGTCCGGCGGTGGCGACGTCTTGCACCCCGTCGCCTCGCCGCTCCCGACCCACCCGCGC CCCCGCCCGCTGCCCCTTCTCCCCCGCCCAGCCGCGGCTCGGCGGCGGCCGCAGCTCCGGAGCAGCTTGA ACAAGACCACGGGAAGGTTGAAGGATAATCTTCTAATACCAGCACTTTGAATGAGAATTTGTTTCTCTGC CACAAACTGATTTTTTTTTTTAAAAAATTTATTTTTCTGGTGGAGGAGTTGAAACAAAGCTAACTTTTGT GGCTTCGCAGCTATGCAGCTTGAAATCCAAGTAGCACTCAATTTTATTATTTCTTACTTGTACAATAAGC TTCCCAGGAGACGTGTCAACATTTTTGGTGAAGAGCTTGAAAGACTTCTTAAGAAGAAATATGAAGGGCA CTGGTATCCTGAAAAGCCATACAAAGGCTCAGGGTTTAGATGTATACACGTGGGGGAGAAGGTAGACCCC GTGATCGAGCAGGCATCCAAAGAGAGTGGTTTGGACATTGACGATGTTCGTGGCAATCTGCCACAGGATT TGAGTGTCTGGATCGACCCGTTTGAGGTTTCCTACCAGATCGGCGAAAAGGGACCAGTGAAGGTGCTGTA CGTGGACGACAGTAGTGAAACCGGCTGTGAGCTGGATAAGGAGATCAAAAACAGCTTTAACCCGGAGGCC CAGGTGTTCATGCCCATAAGCGACCCGGCCTCCTCCGTGTCCAGCTCTCCCTCACCTCCCTTCGGCCACT CTGCTGCTGTCAGCCCTACCTTCATGCCCCGGTCCACGCAGCCTTTAACCTTTACCACTGCCACTTTTGC TGCCACCAAGTTCGGCTCCACCAAAATGAAGAATAGTGGCCGTAGCAGCAAGGTAGCTCGCACTTCTCCG ATCAACCTGGGCCTGACTGTAAATGTGAACGACCTCCTGAAGCAGAAAGCCATCTCTTCCTCAGTGCACT CTCTGTATGGGCTGGGCCTGGGCAGCCAGCAGCAGCCACAGCCGCAGCCACAGCAGCAGCAGCAGCAGCA GCCGTCATCATCCCAGCCGCCGCCTCCACTACCGCAGCAGCAGCAGCAGCAGCCACAGCAGCAGCAGCAG CAGCAGCAGCAAACCTCTGCTCTTTCTCCCAATGCCAAGGAATTTATTTTTCCTAACATGCAGGGTCAAG GTAGTAGTACCAATGGAATGTTCCCAGGTGACAGCCCCCTCAACCTCAGTCCCCTCCAGTACAGCAATGC CTTTGATGTGTTTGCGGCCTACGGAGGCCTCAACGAGAAGTCTTTTGTAGACGGCTTGAATTTTAGCTTA AATAACATGCAGTATTCTAACCAGCAATTCCAGCCTGTTATGGCTAACTAAAAAAACAAAACAAAGGAGA ACATGTATCGTACAAGTTAAAATGCATCGGCCCAAGGGGGAACTTTTTTTCTTTTTTTTTTTTGCCTCCT TGAGATTTTTTTTTTTAAAGCTTATAGTAAGGACACATTCAGGCTTGGTTACAAAATAAAACACGCATCA TTTTTCATTTGCCAACCAAGCACAAAGTTATTTTCTACTGACTGTATATTTTAAAGTATACTTTCAGATA TGGCCTCTTACAGTATTTAAGATATAGCAAGGACATGGCTGAATTTTTTTTTTATAAAAAATTGGCACTA ATAAGTGGGTTTATTGGTCTTTTCTAATTGTATAATTTAATTTAGTACAAAGTTTGTAAAATATCAGAGG ATATATATATATTGTTTCTACGACATGGTATTGCATTTCTATCTTTTTACTACAGTGATCTGTGACAGCT TCATGTTGTATTTTTTTTACTGAAATTGTAAAATATCCATCTTAAAGACATCAGCTATTCTGAAAATTGT GTACAGGATATTCCTTTAGTGGTGGAATTAAAATGTACGAATATTTGCTTTTTCAAAAAAATGTATTTTC TGTTAAAGGTTTAAAGATTTTTGCTATATATTATGGAAGAAAATGTAATCGTAAATATTAATTTTGTACC TATATTGTGCAATACTTGAAAAAACGGTATAAAAGTATTTTGAGTCAGTGTCTTACATGTTAAGAGGGAC TGAAATAGTTTATATTAAGTTTGTATTAAAATTCTTTAAAATTAAAAAAAAAAAAAAAAAAA
Restriction Sites	AscI-NotI
ACCN	BC082588
Insert Size	1089 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC082588, AAH82588
RefSeq Size	2302 bp
RefSeq ORF	1089 bp
Locus ID	22057
Gene Summary	Anti-proliferative protein; the function is mediated by association with deadenylase subunits of the CCR4-NOT complex. Mediates CPEB3-accelerated mRNA deadenylation by binding to CPEB3 and recruiting CNOT7 which leads to target mRNA deadenylation and decay.[UniProtKB/Swiss-Prot Function]

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