2010315L10Rik (BC075695) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Ed2, mED2, D12, Q-SNARE
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC075695 GCGGAATGCAATGACCGGCGAGGGAAGATGAGGAATGGCTTTTCCAGTCCTTTCAACGAATTCTCGCCGG ACATAATCGGCTGTTCAGGGCCTACATGGACTACAGAGACTCTGCTTCAAAAAACGAAAAAGGCCAGATG GCGCAGGCGGAAGGAGCCTATCATCGCCCGCTGGCGACGTCGCGGTTGGAGTTGAACCTGGTACGGCTGC TTTGTCGCTGTGAGTCGATGGCGGCAGAAAAACGAGAACCGGACGAGTGGCGACTGGAGAAGTATGTGGG TGCCCTGGAAGACATGCTGCAGGCCCTGAAAGTTCAAGCAAGTAAACCGGCCTCAGAAGTGATCAGCGAG TACTCCCGCAAAGTGGACTTTCTGAAGGGCATGCTGCAGGCCGAGAAGTTGACCTCCTCCTCTGAAAAAG CTCTAGCTAACCAGTTCCTGGCTCCTGGCCGCGTGCCCACCACAGCCAAGGAGCGGGTGCCTGCCACCAA GACTGTACATTTGCAGTCTAGGGCACGTTACACCAGTGAGATGCGGAGTGAGCTGTTGGGCATGGAACCA TCTGGAGAATGTGAGGTGGACATGAGGAAACGAGCTGCGAAGGGGTCCAGACCTGCAGATGAGAGGCAGT CAGCATCCGAGCTAGACCTCGTCCTCCAGAGGCACCAGGGCCTCCAGGAAAAGCTGGCTGAGGAGATGCT AGGCCTGGCCCGCAGCCTCAAGACCAACACACTGGCTGCCCAGAGTGTCATCAAGAAGGACAATCAGACC CTGTCACATTCACTCAAGATGGCAGACCAGAACCTGGAGAAGCTGAAGCTGGAGTCGGAGAGATTGGAGC AGCATGCACAGAAGTCAGTGAACTGGCTGCTGTGGGCCATGCTCATTGTCGTGTGCTTCGTGTTCATCAG CATGATCCTGTTTATCCGAATCATGCCCAGGCTGAAATAAAACAAGAAAGCCTGCACACCAACAAAAAAA AAAAAAAAAAAAA
Restriction Sites	AscI-NotI
ACCN	BC075695
Insert Size	813 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC075695, AAH75695
RefSeq Size	993 bp
RefSeq ORF	813 bp
Locus ID	67023
Gene Summary	SNARE that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.