Arhgef18 (BC060639) Mouse Untagged Clone

CAT#: MC206181

Arhgef18 (untagged) - Mouse rho/rac guanine nucleotide exchange factor (GEF) 18 (cDNA clone MGC:79199 IMAGE:6849875), (10ug)



CNY 7,120.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AI467246; D030053O22Rik
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC060639
GCGGAGACTGCGAGAGCGACCCGGGAGCCAGCAGGGCGGAGAGCGGCCTGTGAGGGACCCCGCGGAGCCT CCCTGAAGGAGCACCCTCGGACCACACTTCTGTCTGATGGCAGCAGCCCAGCTCCTTCCAGGAATGTCGG CATGACCATCTCTCAGAAAGGGGGTCTCCAGCCAACACCGAGCCCAGCTGGATCTGGGGTGCGACTTGGA CCAATTGCTGGAGACATGGATGAAGCTGACTCCGTGTTTTTAAAGTTGAAGCAGACGGCAGACGACTCTC TGTCACTCACATCCTCGAATGCTGAGTCTGTTTTCATAGAAGACCCTTACATTGCTTCACTGAGGTGTGA GATCGAATCAGATGCACATGAGTTTGAGGCCGAGTCCTGGAGCCTCTCTGTGGATTTAGCATATGCCAAG AAACAGAAGAAGGAGGTGGTAAAGAGACAGGACGTCCTATATGAGCTGATGCAGACAGAGGCTCATCACG TGCGCACACTAAAGATCATGCTGAAAGTATACTCCAGAGCCCTGCAGGAAGAGCTGCAATTCAGTGGCCA GGCAGTCAGCCGTCTGTTTCCGTGCGCTGATGACTTACTGGACATGCACAGCCATTTCCTTGCCCGCCTT AAGGAGCGCCGGCAGGAGTTCCTAGAGGAGGGCAGTGATCGGAATTACGTCATCCAAAAGATCGGAGACG TCCTGGTTCAGCAGTTCTCAGGAGAAACCGGGGAGAGAATGAAGGAGAAATACGCTGTGTTCTGTAGTGG CCACAATGATGCAGTCGGTCATTACAAGCTATTGCTGCAGCAAAGCAAGAAGTTTCAAAACTTAATCAAG AAAATTGGCAACTTTTCCATTGTGCGAAGGCTGGGAGTGCAGGAGTGCATCCTTCTGGTCACACAGCGCA TAACCAAGTACCCGGTGCTTGTGGAACGCATCATCCAGAACACAGAAGCTGGCACTGAGGACTACAAGGA CCTGAGCCAAGCCCTGAGCCTCATCAAAGACATCATCTCACAAGTGGACGCCAAAGTCAGTGAGTATGAG AAGGACCAGCGCCTCAAGGAGATTGCAGCCAAGACGGACCAGAAGTCGTCTGGCAAACTCAAAAACGGGC TAACCTTCCGAAAGGAGGACATGCTGCAGCAGCGCCAGCTCCACCTGGAAGGCGCCCTCTGCTGGAAGTC CACCTCAGGGCGCCTGAAAGATGTCCTCGCTGTCCTGCTGACCGATGTGCTTTTGCTGCTGCAAGAAAAA GATCAGAAATATGTCTTTGCTTCTGTGGACTCAAAGCCACCCGTCATCTCTCTGCAAAAGCTCATCGTGA GAGAGGTAGCCAACGAGGAGAAGGCCATGTTCCTGATCAGCGCCTCCATGCAGGGGCCAGAGATGTATGA GATGTACACCAGCTCCAAAGAGGACAGGAACATCTGGATGGCCCACATCCGCCGGGCCGTGGAGAGCTGT CCTGACGAGGAGGAGGACGTTTTCAGTGAGGCTGAGGAGAAAAAGATAGCTGAAGCCCGCACCATGAAAC TCCAGGAATTTCAAGAGCGACTGAGCCTGAAAGACCAGCTGATTGCCCAGAGCCTTCTGGAGAAACAGCA GATCTACCTGGAGATGGCTCAGCTGAGTGGGCTGGAAGAGTCAGCCCAGAACCGAGGCCTCTTCCGAGGA GGAGGAGACCCCTCTGAGACCCTGCGGGGGGAGCAGATTCTCAGATCGGCCATGAGTGAGATTGAGGGCA TCCAGAGCCTGATCTGCCAGCGGCACCTGGGCAGCACCAGTAGCCAAGTGGAGGAGGGAAGTGTCTCTGC AGGCCTGCCTCGGCGGGCTGAGACCTTCGGGGGCTATGACAGTGTGGGAAGCCCTAGCAAGGGTGGCAGC TTTAAAAGGAAGGTATCCAACAGTGACCTCAGGCCCCAAGACTGGCAGGGACCTGCTAGCAGCCCAGACT CCAGGCCCTGTGACAACAGCGCCCCCAGTGGCTGCTGTGAGGAATCGCCACAGGCTGTGGAGATGCCTAG CACAGAGAGCCTGCCCACTGTCCTGGAATTAGAGCTGGTCCACCGGGTCCAGACACTGTCGCAGTTGCTT CTTAGTCTCCAGGCGGTCATCGCTCAACAGGATAGCTATGTGGAGATGCAAAGAACGGCCATCCAGGAGC GCGAGAAGCAGTTCCGGCTGCAGTCAACACGTGGGAACCTGCTGCTGGAGCAAGAGCGGCAACGCAACTT TGAGAAGCAGCGTGAGGAGCGCGCCGGTGTGGAGAAGCTGCAAAGCCAACTTCGCCAGGAGCAGCAGCGA TGGGAGCGGGAGCGGGCACGCCAGCAGCAGGAACTGGAGCTGGCCGGTGCGCGACTGCAGGAGCGAGAGG GCGAGGCACGCCAGATGCGCCAGCGGCTGGACCAGGAGCGCACCGAGCTAGAGCGACAGCGCCAGGCCTA CCAGCATGACCTGGAGCGTCTGCGTGAGGCCCAGCGAGCAGTGGACCGTGAACGTGAGCGCCTGGAGCTG CTGCGCAGGTTCAAGAAACAGAACACTGTACCAGGGGCACTGCCCCCGGAGGTGCTGGCAGAGGCCCAAC CTGCAAGCCACCCTCCCAGCTTCAATGGCGATGGGCTGGAGGGGCACTCGGCCCCAGCCAAAGCACCAGG AACCCAAGGGAGCGCGATGCTACATGGCACAGGACCTGACAACGTAGAGCGACCCGAGGTGGCCCGCTGG GACAGTGCCCCCCCTGAGAGCCGGCCAGCCAAGAGCGATGTGCCCATCCAGCTACTCAGTGCCACCAATC AGATTCAGAGGCAGACAGCCGTGCAACAGCAGATCCCCACCAAGCTGGCCGCCTCCACCAAGGGTGGCAA GGAGAAGGGCAGCAAGAGCAGGGGTTCCCAGCGTTGGGAGAGCTCAGCGTCCTTCGACCTGAAGCAGCAG CTGCTCCTCAGCAAGTTCATAGGCAAGGATGAGAGTGCATCACGGAACCGCCGTTCACTGAGTCCCGTCC TGCCTGCCGCCCATGGGTCTGCACCTGCCTCAGACCCCTGCTTCCCAGCCCCAAGCCCGGCCCCAGCTGC CACACCCCCCGAGGCCTTCAAGTTCGGAGGCACATCACTACCACCTGTCTCCCCAGCTTCCTCGCTGCCA ACCACACCACTCGCCACCACAGATGAAGTTAGTAAGGAAGATGTCATCTTCTTCTGAGGGGCCATGGCCC CAAGGGCAGACCCCTTTCTGTCCTGATACCCTTTATGTCTCTGGGGATCCTATGGGTGACTGTACTGTCT GGCCGTCCCCTTCCCCTGCACAAGCACAGCCAGGCACTTGTTCCCTTGAGATTGCCCAAATGGTATGCCT TGAGTCTCACAGCTCGCTATAGATTTAGTGGCAAAACATGGGTAGCCTTGAGTCTCACAGCTCGCTATAG ATTTAGTGGCAAAACATGGGTAGCCCCCTTGAACACACATACATACATGCATATATATGTATATGTGTGT GTGTATATATATATATATTATATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites AscI-NotI     
ACCN BC060639
Insert Size 3066 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC060639, AAH60639
RefSeq Size 3568 bp
RefSeq ORF 3066 bp
Locus ID 102098
Gene Summary Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. Also acts as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production. Required for EPB41L4B-mediated regulation of the circumferential actomyosin belt in epithelial cells.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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