Pabpc1 (BC046233) Mouse Untagged Clone

CAT#: MC206148

Pabpc1 (untagged) - Mouse poly A binding protein, cytoplasmic 1 (cDNA clone MGC:58087 IMAGE:6816124), (10ug)



CNY 4,744.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms PABP, PabpI
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC046233
CGGCGGTTAGTGCTGAGAGTGCGGAGTGTGTGCTCCGGGCTCGGAACACACATTTATTATTAAAAAATCC AAAAAAAATCTAAAAAATCCTTTGAAAAAAACCCAAAAAAAATTTACAAAAAAATCCGCGTCTCCCCCGC CGGAGACTTTTATTTTTTTTCTTCCTCTTTTATAAAATAACCCGGTGAAGCAGCCGAGACCGACCCGCCC GCCCGCGGCCCCGCAGCTCCAAGCAGGAACCCAGAGACCGAGGCCTTCCTGCCGCCCGGACCCAGCACCG CCAGCCTCGCTCCCCCGGTTCCACGGCCGTTGAGTACGCGTCGATTCCGGTTGAATTTTGTCCCTCTGCG CTCGCCCCCGCTCCCCTCCCCCCGGCTCCGTCCCCCGGCCCCGCACTCGCTCTCCTCCTCTCACGGGAAA GGTCGCGGCCTGCGGCCCCGCGGGCAGCCGTGCCGAGATGAACCCCAGCGCCCCCAGCTACCCCATGGCC TCTCTGTACGTGGGGGACCTGCACCCCGACGTGACCGAGGCGATGCTCTACGAGAAGTTCAGCCCGGCCG GGCCCATCCTCTCCATCCGGGTCTGCAGGGACATGATCACCCGCCGCTCCTTGGGCTACGCGTACGTGAA CTTCCAGCAGCCGGCGGACGCGGAACGTGCTTTGGACACCATGAATTTTGATGTTATAAAGGGCAAGCCA GTACGCATCATGTGGTCTCAGCGTGATCCATCACTTCGCAAAAGTGGAGTAGGCAACATATTCATTAAAA ATTTGGACAAATCCATCGACAATAAAGCACTATATGATACGTTTTCTGCGTTTGGTAACATCCTTTCATG TAAGGTGGTTTGTGATGAAAATGGCTCCAAGGGCTATGGATTTGTACACTTTGAAACACAGGAAGCAGCT GAAAGAGCTATTGAAAAAATGAATGGGATGCTTCTAAATGATCGTAAAGTGTTTGTTGGACGATTTAAAT CTCGGAAGGAACGAGAAGCAGAGCTTGGAGCCAGGGCAAAGGAGTTCACCAATGTTTACATCAAGAACTT TGGAGAAGACATGGATGATGAGCGCCTTAAGGAACTCTTTGGCAAGTTTGGGCCTGCCTTAAGTGTGAAA GTAATGACAGATGAAAGTGGAAAATCCAAAGGATTTGGATTTGTAAGCTTTGAAAGGCATGAAGATGCGC AGAAAGCTGTGGATGAGATGAATGGGAAGGAGCTCAATGGAAAACAGATTTATGTTGGACGAGCTCAGAA AAAAGTGGAACGGCAGACGGAACTTAAGCGCAAATTTGAGCAGATGAAGCAAGATAGGATCACCAGATAT CAGGGTGTGAACCTTTATGTGAAAAATCTTGATGACGGGATTGATGATGAGCGTCTCCGGAAGGAGTTTT CTCCGTTTGGTACAATCACCAGTGCAAAAGTAATGATGGAGGGTGGGCGCAGCAAAGGGTTTGGTTTTGT ATGTTTCTCATCCCCTGAAGAAGCCACTAAAGCAGTTACAGAGATGAATGGTAGAATTGTGGCCACGAAG CCACTGTATGTAGCTTTAGCTCAGCGCAAAGAAGAGCGCCAGGCTCACCTCACTAACCAGTATATGCAGA GGATGGCAAGTGTACGAGCTGTGCCCAACCCCGTGATCAACCCCTACCAGCCAGCACCTCCTTCAGGTTA CTTCATGGCAGCTATCCCACAGACTCAGAACCGTGCTGCATACTATCCTCCTAGCCAAATTGCTCAACTA AGACCAAGTCCTCGCTGGACTGCTCAGGGTGCCAGACCTCATCCATTCCAGAATATGCCCGGTGCTATCC GCCCAGCTGCTCCTAGACCACCATTTAGTACGATGAGACCAGCTTCCTCACAGGTTCCACGAGTCATGTC AACACAGCGTGTTGCTAACACATCAACACAGACAATGGGTCCACGTCCTGCAGCTGCTGCTGCTGCAGCC ACTCCTGCTGTCCGCACCGTTCCCCAGTATAAATATGCTGCGGGAGTCCGCAATCCCCAGCAACATCTTA ATGCACAGCCACAAGTTACCATGCAACAGCCTGCTGTTCATGTGCAAGGTCAAGAACCTTTAACTGCTTC CATGTTGGCATCTGCGCCCCCGCAAGAGCAGAAGCAAATGTTGGGTGAACGGCTGTTTCCTCTTATCCAA GCCATGCACCCTTCTCTTGCTGGTAAAATCACTGGCATGCTGTTGGAGATTGATAACTCAGAATTACTTC ACATGCTCGAGTCTCCAGAGTCTCTCCGCTCAAAGGTTGATGAAGCTGTAGCTGTACTACAAGCCCACCA AGCGAAAGAGGCTGCCCAGAAAGCAGTGAACAGTGCCACTGGTGTTCCAACTGTCTAAATTGATCAGGGA CCACGAACAGAAACTCGTGCTTCACCGAAGAAAAATATCTTAAACATCGAAAAATTTAAATATTATGAAA AAAAACATTGCAAAATATAAAATAAATAAAAAAAGGAAAGGAAACTTTGAACCTTATGTACCGAGCAAAT GCCAGGTCTAGCAAACAGTGCTAGTCCTAGATTACTTGATTTAAAACAACAAAAAAATACAAAAAAATAG TAAAATATAAAAACAAATTAATGTTTTATAGACCCTGGGAAAAGAATTTTCAGCAAAGTACAAAAATTTA AAGCATTCCTTTCTTTAATTTTGTAATTCTTTACTGTGGAATAGCTTGGGACATCAGCTCTGTTTTAAGG AGCAGAACTGATGACTGAGCAAGGAAACGTAATTTGGATTATAAAATTCTTGCTTTAATAAAAATTCCTT AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites AscI-NotI     
ACCN BC046233
Insert Size 1911 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC046233, AAH46233
RefSeq Size 2834 bp
RefSeq ORF 1911 bp
Locus ID 18458
Gene Summary Binds the poly(A) tail of mRNA, including that of its own transcript. May be involved in cytoplasmic regulatory processes of mRNA metabolism such as pre-mRNA splicing. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed (By similarity). By binding to long poly(A) tails, may protect them from uridylation by ZCCHC6/ZCCHC11 and hence contribute to mRNA stability (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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