Nup85 (BC079856) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	frount; Pcnt1
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC079856 GAGGCCTGAGCGCAGAGAAGCGGCCGCGCGGCAGGGTCCCAGCCGCGGGGGGCACGGCGCTATGGAGGAG CTCGACTGCGAGCCCGCAGTAACTTGGATTCCAGGTGTGAATTCCAAGAAGAAGCAAATGTGTTTTGACT GGGGCCCCGGCGAGATGCTTCTGTGTGAAACTTCCTTCAACCAAACAGGCAAATCAGAGAAGGTGCCGAG CTGCCCTTTTATCTACATCATACGGAAAGATGTGGATGTTTACTCTCAAATTTTGAGAAAACTCTTCAAT GAATCCCATGGAATCTTTGTTGGCCTGCAAAAAATTGAGGAAGAACTGTCGGGGAAGTCCAGGAAAGCTC AATTGGTTCGAGTGAGTAAAAATTACCGTTCAGTCATACGGGCCTGTATGGAAGAAATGCATCAGGTTGC AATTGCTGCTAAAGATCCAGCCAGTGGCCGGCAGTTCAGCAGCCAGGTCTCCATTTTGTCAGCCATGGAG CTCATTTGGAACCTGTGTGAGATTCTCTTTATTGAAGTAGCACCAGCTGGCCCTCTCCTCCTTCACCTTC TTGACTGGGTCCGACTGCACGTGTGCGAGGTGGACAGTTTGTCGGCAGATGTCCTGGGCGGTGACAACCC AAGCAAGCATGAAAACTTCTGGGACCTGGTGACTGTTCTGGTGCTTCAGGGCCGGCTCGATGAGGCACGG CAGATGCTAGCCAAAGAAGCTGATGCCAACCCCTCTTGTGCAGGCATGTGCCGCGTCCTTGGGGACCTGA TGAGGACAATGCCCATTCTCAGCCCTGGCAATACTCAGACACTGACAGAGTTGGAGCTGAAGTGGCAGCA CTGGCGTGAGGAGTGTGAAAGACACTTACAAGACAACACATTTGCAGCCAACCCCCGTCTGGAGTCTCTC TGCAAGATCATGCTGGGAGATGAGGCCGCCTTGTTGGAGCAGAAGGAGCTTCTGAGCAACTGGTACCATT TCTTAGTGACGAGGCTGCTGTACTCTAACCCCACAGTGAAGCCCATTGACCTGCACTTCTATGCCCAGTC CAGCCTAGACATGTTTCTTGGAGGTGAGAGCAGTCCAGAACCACTGGACAACATCTTGATGGCGGCCTTT GAGTTCGACATTCACCAGGTGATCAAAGAGTGCAGCATCGCCCTGAGCAACTGGTGGTTCGTAGCTCACC TCACAGACCTTTTGGATCACTGCAGACTCCTCCAGTCACACAATCTCTATTTTGGTTCTAACATGAGAGA ATTCCTCCTGCTGGAGTACGCCTCAGGACTGTTTGCTCACCACAGCCTGTGGCAGCTGGGGGTGGACTAC TTTGATTACTGCCCGGAGCTAGGCCGAGTTTCCTTGGAGCTGCACATTGAGCGGATTCCTCTCAACACAG AGCAGAAAGCCTTGAAGGTGCTGAGGATTTGTGAGCAGCGGCAGATGACTGAGCAAGTTAAAAGCATCTG TAAGATCTTGGCCATGAAGGCTGTTCGTAATAACCGCTTGGGCTCAGCACTCTCCTGGAGCATCCGTGCC AAAGATGCTGCCTTTGCCACACTCGTATCTGACAGATTCCTCCGGGATTACTGTGAGAGAGGCTGCTTTT CTGACTTGGATCTCATTGACAATCTGGGGTCAGCCATGATGCTCAGTGATCGACTGACGTTTCTGGGAAA GTACCGGGAGTTCCACAGACTGTACGGGGAGAAGCGCTTTGGTGACGCTGCTTCTCTTCTGCTGTCCCTC ATGACCTCTCAGATTGCACCTCGTTCTTTCTGGATGACTCTGCTCACAGATGCCCTTCCTCTTTTGGAAC AGAAACAGGTGATTTTTTCAGCAGAGCAGACATATGAGCTGATGCGGTGCCTGGAAGACTTGGCCTCAGG GAGGCCAGAGTGTGGTGAACCTGATGCCCAGCGACTGCAGGATGACGACATAGAGACCACCAAGGTGGAG ATGCTGAGACTGGCTCTTGCCCGGAATCTTGCTCGGGCAATTATAAGGGAAGGCTCACTGGAGGGTTCCT GAGAGAAGATGAGGACAGTTGCAATATAGTAACTTTGTATGGCAATGTAAATAGATTTTAAAGAATAAAT TGTTTTGCAAATATAAAAAAAAAAAAAAAAAAA
Restriction Sites	AscI-NotI
ACCN	BC079856
Insert Size	1971 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC079856, AAH79856
RefSeq Size	2133 bp
RefSeq ORF	1971 bp
Locus ID	445007
Gene Summary	Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP96/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2-activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl-inositol 3-kinase-Rac-lammellipodium protrusion cascade. Involved in nephrogenesis.[UniProtKB/Swiss-Prot Function]

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