C1qb (BC067001) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC067001 GGCTCTGGGCTCTGGGAATCCACTGCTGTCCGGCCTAGAAGCATCACAGAACACCAGGATTCCATACACA GGAAGCCCCTGAGGCTGAGCTGATGAAGACACAGTGGGGTGAGGTCTGGACACACCTGTTACTGCTGCTT CTAGGTTTTCTCCATGTGTCCTGGGCCCAAAGCAGCTGCACCGGGCCCCCTGGCATCCCTGGCATCCCTG GGGTCCCTGGGGTTCCTGGCTCTGATGGCCAACCAGGCACTCCAGGGATAAAGGGGGAGAAAGGGCTCCC TGGACTGGCTGGAGACCTTGGTGAGTTTGGAGAGAAAGGGGACCCAGGGATCCCTGGGACTCCAGGCAAA GTTGGCCCTAAGGGTCCCGTCGGCCCTAAGGGTACTCCAGGCCCCTCTGGACCCCGCGGTCCCAAAGGCG ATTCTGGGGACTACGGGGCTACACAGAAAGTCGCCTTCTCTGCCCTGAGGACCATCAACAGCCCCTTGCG ACCGAACCAGGTCATTCGCTTCGAAAAGGTGATCACCAACGCGAACGAGAACTATGAGCCACGCAACGGC AAGTTCACCTGCAAGGTGCCTGGCCTCTACTACTTCACCTATCATGCCAGCTCCCGGGGCAACCTGTGTG TGAATCTCGTTCGTGGCCGCGATCGGGACAGCATGCAGAAAGTAGTCACCTTCTGTGACTATGCCCAGAA CACCTTCCAGGTGACCACAGGTGGGGTAGTCTTGAAGCTAGAGCAAGAGGAGGTTGTTCACCTGCAGGCC ACAGACAAGAACTCCCTCCTGGGCATTGAGGGTGCCAACAGCATCTTCACTGGCTTTCTGCTTTTCCCTG ACATGGATGCGTAATCACGGGGTCAAATTACACCTATCCAACACCATCTTCCTGCCTCCCTGCCAGCAAT CCTCCCTGGACCCCTGACATCACCCCCTTGACTGCCTGAAACCCAGACCAGAGCCCTGTAGATGTTACAG AACGAATGGGTCAATAAACTCTTCAAGGCCAAAAAAAAAAAAAAAAA
Restriction Sites	AscI-NotI
ACCN	BC067001
Insert Size	762 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC067001, AAH67001
RefSeq Size	1027 bp
RefSeq ORF	762 bp
Locus ID	12260
Gene Summary	C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.[UniProtKB/Swiss-Prot Function]

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