Cfl1 (BC058726) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	cofilin
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC058726 TCGGATCCCGGCAGCAGCTGCGGCGGCTCAGGTCTTCAGTTGCTCTTTCTGTCTCCCTTTCGTTTCCGGA AACATGGCCTCTGGTGTGGCTGTCTCTGATGGTGTCATCAAGGTGTTCAATGACATGAAGGTTCGCAAGT CTTCAACACCAGAAGAAGTGAAGAAACGCAAGAAGGCGGTGCTCTTTTGCCTGAGTGAGGACAAGAAGAA CATCATCCTGGAGGAGGGCAAGGAGATCCTGGTAGGAGATGTGGGGCAGACTGTGGACGACCCCTACACC ACTTTTGTCAAGATGCTGCCAGACAAGGACTGCCGCTATGCACTCTATGATGCAACCTATGAGACCAAGG AGAGCAAGAAGGAGGACCTGGTGTTCATCTTCTGGGCCCCCGAGAATGCACCCCTCAAGAGCAAAATGAT CTATGCCAGCTCCAAGGATGCCATCAAGAAGAAGCTGACAGGAATCAAGCATGAATTACAAGCTAACTGC TACGAGGAGGTCAAGGACCGCTGCACCCTGGCAGAGAAACTAGGTGGCAGCGCCGTCATTTCCCTGGAGG GCAAGCCTTTGTGAGCCACCTCCAGCCCCCTGCCTGGAGCATCTTAACAGCCCCAGACCTGCTCTTGGGT GTTGCAGGCTGCCCCTTTCCTGCCAGACCGGAGGGGCTGGGGGGATCCCAGCAGGGGGAGGGCTATCCCT TCACCCCAGTTGCCAAACATCCCTCCCACCCCCTGGACCGTCCTTCTCCCTCCATCCCTGACGGTTCTGG CCTTCCCAAACTGCTTTTGATCTTCTGATTCCTCTTGGGTTGACGCAGACCAAGTCCCGTCCTAGGCACC CAGTTTGGGGGGAGCCTGTATTTTTTTTTTTAACGACACCCCTACTCCGTATCCCTCCCCATCCCATGCT GCCAACTTCTAACCACAATAGTGACTCTGTGCTTGTCTGTTTAGTTCTGTGTGTAAATGAAATGTGGAAA TGACCCTCCCTGCCCCAGCTGGCTGCCCTCCCCTTTCCTTTGATCTTGACCACTCATGGAAGCAGGACCA GTAAGGGACCTTCAATTTAAAACAAAACAAAAAAAAAAAAAAAAAA
Restriction Sites	AscI-NotI
ACCN	BC058726
Insert Size	501 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC058726, AAH58726
RefSeq Size	1096 bp
RefSeq ORF	501 bp
Locus ID	12631
Gene Summary	Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11809832). Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (By similarity). Required for neural tube morphogenesis and neural crest cell migration (PubMed:15649475).[UniProtKB/Swiss-Prot Function]

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