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Cnr1 (BC079564) Mouse Untagged Clone
CAT＃: MC206086
Cnr1 (untagged) - Mouse cannabinoid receptor 1 (brain) (cDNA clone MGC:90716 IMAGE:30536039), (10ug)
CNY 3,656.00
货期*
现货
规格
| Cited in 1 publication. |
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | CB-R; CB1; CB1R |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC079564
CCATGGCTGAGGGTTCCCTCCTGGCACCTCTTTCTCAGTCACGTTGAGCCTGGCCTAATCAAAGACTGAG GTTATGAAGTCGATCTTAGACGGCCTTGCAGATACCACCTTCCGTACCATCACCACAGACCTCCTCTACG TGGGCTCAAATGACATTCAGTACGAAGATATCAAAGGAGACATGGCATCCAAATTAGGATACTTCCCACA GAAATTCCCTCTAACTTCCTTCAGGGGTAGTCCCTTCCAAGAAAAGATGACGGCAGGAGACAACTCCCCG TTGGTTCCAGCAGGAGACACAACCAACATTACAGAGTTCTATAACAAGTCTCTCTCATCGTTCAAGGAGA ACGAGGACAACATCCAGTGTGGGGAGAATTTTATGGACATGGAGTGCTTCATGATTCTGAATCCCAGCCA GCAGCTGGCCATCGCTGTCCTGTCCCTCACCCTGGGCACCTTCACGGTTCTGGAGAACCTGCTGGTGCTA TGTGTCATCCTTCACTCCCGCAGTCTCCGATGCAGGCCTTCCTACCACTTCATTGGCAGCCTGGCGGTGG CCGATCTCCTGGGAAGTGTCATCTTTGTCTACAGCTTTGTTGACTTCCACGTGTTCCACCGCAAAGATAG TCCCAATGTGTTTCTGTTCAAACTGGGTGGGGTTACCGCCTCCTTCACAGCATCTGTGGGCAGCCTGTTC CTCACGGCCATCGACAGGTACATATCCATTCACAGGCCTCTGGCCTATAAGAGGATCGTCACCAGGCCCA AGGCCGTAGTGGCCTTTTGCTTGATGTGGACTATTGCAATAGTAATTGCTGTGTTGCCTCTCCTGGGCTG GAACTGCAAGAAGCTGCAATCTGTTTGCTCAGACATCTTCCCACTCATTGATGAAACCTACCTGATGTTC TGGATCGGAGTCACCAGTGTGCTGTTGCTGTTCATTGTGTATGCATACATGTACATTCTCTGGAAGGCTC ACAGCCACGCAGTTCGCATGATCCAGCGTGGAACCCAGAAAAGCATCATCATTCACACCTCAGAAGATGG CAAGGTGCAGGTGACACGGCCTGACCAAGCCCGCATGGACATTAGGCTGGCCAAAACCCTGGTTCTGATC CTGGTGGTGTTGATCATCTGCTGGGGCCCTCTGCTTGCGATCATGGTGTATGATGTCTTTGGGAAGATGA ACAAGCTTATCAAGACGGTGTTTGCCTTCTGTAGTATGCTCTGCCTGCTGAACTCCACCGTGAACCCCAT CATCTATGCTCTGAGGAGCAAGGACCTGAGACATGCTTTCCGCAGCATGTTCCCTTCATGTGAAGGCACT GCGCAGCCTCTAGATAACAGCATGGGGGACTCAGACTGCCTGCACAAGCACGCCAATAACACAGCCAGCA TGCACAGGGCCGCGGAAAGCTGCATCAAGAGCACTGTTAAGATCGCCAAGGTGACCATGTCTGTGTCCAC AGACACGTCTGCCGAGGCTCTGTGAGCCTGCTGCTTTTGTGGCTGCCCAGAAAAAGAAAATTTTTTTTTT TAGCTTAAAATTTAGAAGTCTGTTGTCTCATCGGTTATATATTTTTTAAGTTTACCATGTTTAGTAAAAT AGTGACTGTCACCGTGCTTACTTAGTTTGCTGACATCTCAGTAGTTTAGGTACTTAAACTCCATTCCCCA GGGGTTTACAGTGAAGAAAGTCTATCGCTGCAGTTGCTCGGCGATCCTTCAAAGTCTCTGAAATAGAAGG GAAAACGTTGGCTTCCAAATCTGAAGCCTAACTACCCATAGAAAACCACCATGAAATAAGTAATGCCTTT GTAACGACAACTTTAATGATGCTGTGTCTGTCCATAGTATAAAAGGTACATTTTTACAATAAGTATAGTG CTAAAGTAGAGATACTTTGTAACATGTTTTCTGTCCTGTTAGTTGTGTGTCAGTGTTTACTGTGTCTTTT TTTTTTTTTTTTTTTTTGGTCTAGCTTAGCAAAAACAGAATATAGCCCTCATAAGAGCTATGGCATACAG GAGTGGTGATGTAGCAGGGTGACCCCTGTTTAGATAGTATTACTGTTCATGAGACAATTTTAGAAACCTT AGTATTTCTTCCAATATCCGTATCTAAGTTAATATTGAAATAAGCATAAAGGTTTATTTTTCTGTTAAAA CCACAAGAAAAATTTGAAGACTGTTCAAAGTATTGAGCAGAATTCAAGGACACTTAAAACTGTGTTAGGC CTGCATTTTCATATGAGGACATTCCATATCTTCTGGACCACAGCTGTTCAGTTGGATACTGGATTATAAT CAGAATGGAAAAGAGAAAGCACATTGACTTTTAAAGCTGACATTCTGACTTCCTGCAGTTGTTGGCTGTA ACTAGACCTCTTCAGACAGCATGTGTCAATCTTAGTGTACACTGTGACCACTGTGCAGTTGCTGTTTCCT TGACGTGTATTGCATTGTTATGTTCTGGATTTAAGTAGATTTCAGGCCTCGATGGCCAAAAACCAGTTGT CTTCTTCTTGAGAAGAAATATTGTCTGACTCAGTAAAATTGTGTGTGTGATTGTAAGTGTATATGTGTGT GTATGTGTGTGTGTGTGTGTTTGTGTTACTCTATCTTGTAACTGTTACAGAAATGTCCCCCATGAGGAAA CTGACCAAGGGTAAACAGTACCACTTGCTGCGCTCTTGCACATGAACTTAGCTTCTACAGTTGAGCTCTT CATGAAATCCTGTTTATAAAAATTCTGACTAAATCTTTTCAGAAGAATCTTCCCACCTAGGAGACCGGAT TAAGCATTCTAATGCCATTTAGGTGTTCTGTAAGGCCAGCGTTCAGCAAGAAGTCATCAGTAAGAGGTGC CAGAAAATAGAGGATTCTCCCAGGAAGAGGGGAGTGTGGAGGATGGTGGGCGCCTACTAATAATCAAGTC CATGGAAATGGAAATCAGTAAAGGGGTTGGTTCTAAGACCTATACCCACACCCCTACAGTAGTAAGTTTC AGTGGTGCATGTCAGCGGTGCAATCTCAGGTTAAGTAACAACATGCCAGCTGGTCCAGGCCATTTCATTA CAGAGGGTGACAGCCCAGTACCTGGCTCTGTTTGGAATGATTGGGCTAAGGAAAAGGTAGTACCTCCTCT ACTTGCTGGTATCAAGATGTGCCAAAACATAAAGATGAGGGTGGCTAGCTTCTGATGACATCTCTGCCGT GGTACTCAGAATTGGGCCCTTTTCCAATAAAGAGAAGTATTGCTTTTAGGAAGCCACTCTATCCCGATAA GTTCTACATAGAGTCTCTCCTTGTTCAGGTTTTCACCAGCCCTAAGAACTGCATGGCATGAAGTGGGGAG CTAGCATCTGTTGGTGATTTCTACCATTTGGGGTTCCCAGCCTGACAGGGCAGTACCCCTTCTTGCAATC AGTGTGACCTGAGGGCCAAACTAGGATATGTCTAGATTTGGCCTGCTGCAGTCTCTTGCTTTTCTTCTCT GTGCACAGAGGAGGGCTCCGGATGCTGGAGTATTAGTAACTCCTTAGAGAAGGGTATTGAGAGAATACCA CCAGGGGCTAGGAATTGTCAGCTTCGTCCTACATGAACCTGAATATCAACCGACATGTCTCTAGCTACCA GGCAGTACCATCTCTTCTCACAGCTACAAAGACCTGGTAGGAAATTCTAGGTATTTAAAAAAAAATCCAA GATTCACTTTTAGAACTGTATTTGTGTAAATGCCATTTTAGTGATAAGATTTTATAGTGTATTGAACTTT CAAGACCTAACTCATATTTAATAAGCTAAGGGACAATGGGGCTGATAGCACTAAACTTGGTGCTTATTGA TACTCTAAGAAATATCTGTGAAATATCATCATGTGTGCGTTAAGCTACCTTCATTTAAAAGGACTTAAAA TAAGTTGGATCCACTTTGGCTTTGACCATATCATTTCCTAACCCTACTGACCAAAACATTCTCCCTAGTG AGTATTAGTCATTAGAATTGCATTCATTAGTATCATTAATTAACTAGGCCCTTAATTAGATTCATTAATT TAAATGATTTTAATTTGATCACTTATGTACTCTGGCATCAGGGTTATCTGTTTCCCTCTGACATGTGTCC TGAACATGCATTCTTCAGCCATTACTCATCTTAAAAAGGGGCCAATTCCCAAACTGCTTGACTTCATCCA AACAAAGACTGGGTCCTAGACTAGCAGTATCAGTTAAACAAAGAGAGCTGTCCCTAAGAGCCCCTAGTTT TTCAACTATGTATTAAGTGCATGTAAAAATCAGTGTGCGTCTGGGCAGCGTAGACATATGTCTGTGCCAG ATGACTCCAAAGCTGTCGGATACTGTCAGGTTTAGCTGGTATCAAATGCCAAGGAATTTTTGCTACCTAA AACCATCTGCATAAAATAGGCCCAATTACCAGATGAGAACTAGGTCTCTAGTTACCATTCAGTTCATTAA TTTCTGCCATTCCATACCCATAAACAGGACCACTAACATCATGCACAAAATTAGATTCCTCATACTCTTG ACTGTATATTTGTATGATATTTTAAAATCTCCTAAATGGCCATTCACAGTTGCTTTGCACTGGCCTTCCA GTAAAATGTTAACACAGCTATTGTAATATACACAAAGCATTCTATCTACTGATTTGGACTGAATGTATGT ACATAGGTTTTACAAAAAAAAAAGTCAGATGTTAAAGCAGTGGTTTACAGATCAGTGATTTTCAGATAGA GTTTCTTTCAGTTGCTGTGGAATCTTTAAAAAGCACTCTTAGTGTGAACTTACTGAACTCTAGGGGTCCT GCGATCAGAGTCCCCTAGAGAGGAAACCTTTTCTTGTAATAATATGAACCAAGATACCAGATTATCTTGA GTCTCACTTCCAGTGTTTCAAGTGGAAAACGTATCTCCTTATTGTCTCTGTAAATGGAACATCCTTACTT TTTTCTCTTAAAGAATATTGTACTGTTAGATGTTTGTTGAACTGGTAGCATCATTGAGACTGCTGTGATG TCTTTGTCAGTAATCTGTATAATATTTTGTATACAAGTACTGGTAATATTGTTATTAAATGTAGCTCGGT CATTAAATTACTATAGCAAAGTAGTACTTCTGTAATATTTACAATGTATTAAGCCTACAGTATATTTTAT TTCATTGATGTAATTTAATTGTTATTTATTCAAGAGAAAGCAGTTCATCATGTCTATTGTCTAAAATTAC CTGGAATCAAATAAAAATTCTAGATTATCATGAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | AscI-NotI |
| ACCN | BC079564 |
| Insert Size | 1422 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC079564, AAH79564 |
| RefSeq Size | 5302 bp |
| RefSeq ORF | 1422 bp |
| Locus ID | 12801 |
| Gene Summary | G-protein coupled receptor for cannabinoids, including endocannabinoids (eCBs), such as N-arachidonoylethanolamide (also called anandamide or AEA) and 2-arachidonoylglycerol (2-AG) (PubMed:9888857, PubMed:22388959). Mediates many cannabinoid-induced effects, acting, among others, on food intake, memory loss, gastrointestinal motility, catalepsy, ambulatory activity, anxiety, chronic pain (PubMed:9888857, PubMed:27828947). Signaling typically involves reduction in cyclic AMP (PubMed:8832654, PubMed:27828947). Signaling typically involves reduction in cyclic AMP (By similarity). In the hypothalamus, may have a dual effect on mitochondrial respiration depending upon the agonist dose and possibly upon the cell type. Increases respiration at low doses, while decreases respiration at high doses (PubMed:25707796, PubMed:27828947). At high doses, CNR1 signal transduction involves G-protein alpha-i protein activation and subsequent inhibition of mitochondrial soluble adenylate cyclase, decrease in cyclic AMP concentration, inhibition of protein kinase A (PKA)-dependent phosphorylation of specific subunits of the mitochondrial electron transport system, including NDUFS2 (PubMed:27828947). In the hypothalamus, inhibits leptin-induced reactive oxygen species (ROS) formation and mediates cannabinoid-induced increase in SREBF1 and FASN gene expression (PubMed:25869131). In response to cannabinoids, drives the release of orexigenic beta-endorphin, but not that of melanocyte-stimulating hormone alpha/alpha-MSH, from hypothalamic POMC neurons, hence promoting food intake (PubMed:25707796). In the hippocampus, regulates cellular respiration and energy production in response to cannabinoids. Involved in cannabinoid-dependent depolarization-induced suppression of inhibition (DSI), a process in which depolarization of CA1 postsynaptic pyramidal neurons mobilizes eCBs, which retrogradely activate presynaptic CB1 receptors, transiently decreasing GABAergic inhibitory neurotransmission (PubMed:22388959). Also reduces excitatory synaptic transmission (PubMed:27828947). In superior cervical ganglions and cerebral vascular smooth muscle cells, inhibits voltage-gated Ca(2+) channels in a constitutive, as well as agonist-dependent manner (By similarity). In cerebral vascular smooth muscle cells, cannabinoid-induced inhibition of voltage-gated Ca(2+) channels leads to vasodilation and decreased vascular tone (By similarity). Induces leptin production in adipocytes and reduces LRP2-mediated leptin clearance in the kidney, hence participating in hyperleptinemia (PubMed:22841573). In adipose tissue, CNR1 signaling leads to increased expression of SREBF1, ACACA and FASN genes (PubMed:15864349). In the liver, activation by endocannabinoids leads to increased de novo lipogenesis and reduced fatty acid catabolism, associated with increased expression of SREBF1/SREBP-1, GCK, ACACA, ACACB and FASN genes (PubMed:15864349, PubMed:21987372). May also affect de novo cholesterol synthesis and HDL-cholesteryl ether uptake (PubMed:21987372). Peripherally modulates energy metabolism. In high carbohydrate diet-induced obesity, may decrease the expression of mitochondrial dihydrolipoyl dehydrogenase/DLD in striated muscles, as well as that of selected glucose/ pyruvate metabolic enzymes, hence affecting energy expenditure through mitochondrial metabolism (PubMed:26671069). In response to cannabinoid anandamide, elicits a proinflammatory response in macrophages, which involves NLRP3 inflammasome activation and IL1B and IL18 secretion. In macrophages infiltrating pancreatic islets, this process may participate in the progression of type-2 diabetes and associated loss of pancreatic beta-cells (PubMed:23955712).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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A synthetic cannabinoid MAM-2201 potently suppresses synaptic transmission via activation of presynaptic CB1 receptors and reduces synaptically-evoked Intracellular Ca(2+) transients in cerebellar Purkinje cells
,Irie, T;Kikura-Hanajiri, R;Usami, M;Uchiyama, N;Goda, Y;Sekino, Y;,
Neuropharmacology
,PubMed ID 25747605
[Cnr1]
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