Siah2 (BC058400) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AA415433; Sinh2
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC058400 GCTGGAGTCGGTGCGGGTCGGCGTAGGTCCCCTGGGAAGGGTCGGGAGACTCGGGCTACGTCCTTTCCCT GGGCTGCCGGCTTCCACTGTCGCAGAGAAGCGACCCCACGCGGCGGCGATCGACTCATTCAAGGGCTCCC AGCGAGGAAACCGCTCGGGCGCTGCCAGCTGGGTCGGGCGGAGCTGCGCGGGCCGCAGGGTGGCTCTGCC AGTCCCTGAGTGCCGGGTCGGCCGCACACGTGTCTTAGCGTCACGTCCGCGCGCGCCCCCGGGGCTTGCG TCAGCTGCCGCTCCTGAAGCGAGTGGGCCAGGGCTCTGCGCACCAGAACGCCGCCGGAGGAGGGCACCGC ACGGCCGCGAGCCCCGAGAGCGACGACCATCCGGGTGCCGCCGAGAGGATCGGCGCGGTCCGGTCCCCGC CCTCCCGGTGCCGGTCGGGTCCGCGCTGGGTCCCCCGGGCTGGGCGCCGTGGCGTTGCCCCGGAGCCCGC CACCCGCGGGGCGCGCACCGCCTTAACCCGGGCGGCCCCGCGGCAGGCAGACGCCCGCAGGTCCATGGTG GGCTCGGAGCGCGATGAGCCGCCCGTCCTCCACCGGCCCCAGCGCTAACAAACCCTGCAGCAAGCAGCCG CCGCCGCCTCAGACCCCGCACGCCCCGTCCCCGGCTGCGCCCCCGGCCGCCGCCACCATCTCGGCAGCGG GCCCCGGCTCGTCCGCGGTGCCCGCCGCGGCAGCGGTGATCTCAGGCCCCGGAGCCGGCGGCGGGGCCGA CCCGGTGTCCCCGCAGCACCACGAGCTGACCTCGCTCTTCGAGTGCCCGGTCTGCTTTGACTATGTCCTG CCCCCCATTCTGCAGTGCCAGGCCGGGCACCTGGTGTGTAACCAATGCCGCCAGAAGTTAAGCTGCTGCC CGACGTGCAGGGGCGCCCTAACGCCCAGCATCAGGAACCTGGCTATGGAGAAGGTCGCCTCGGCAGTTCT GTTTCCCTGTAAGTATGCTACCACGGGCTGTTCCCTGACTCTACACCATACAGAGAAACCAGAGCATGAA GACATCTGTGAATACCGTCCTTATTCCTGTCCTTGTCCTGGTGCGTCCTGCAAGTGGCAGGGATCCCTGG AAGCTGTGATGTCCCATCTCATGCATGCCCACAAGAGTATCACTACCCTTCAGGGAGAGGACATAGTCTT TCTAGCTACAGACATTAACCTGCCAGGGGCTGTGGACTGGGTGATGATGCAGTCCTGTTTTGGCCACCAC TTCATGCTGGTACTTGAAAAGCAAGAGAAGTACGAAGGCCACCAGCAGTTCTTTGCCATCGTCCTCCTCA TTGGCACCCGAAAGCAAGCTGAGAACTTTGCCTACAGACTGGAGTTGAATGGGAACCGGAGGAGACTGAC CTGGGAGGCCACACCTCGGTCCATTCATGATGGCGTGGCTGCAGCCATCATGAACAGTGACTGTCTTGTT TTTGACACAGCCATAGCACATCTCTTTGCAGATAATGGGAACCTTGGAATCAATGTCACGATATCTACAT GCTGTCAGTGAGGCTCCAGGAGGCTTTCCTAACCCTGGGAAGTTATTTGGGCATAGCGCTCTATGTTTAA TAAAGGTTTTTTAATAGATGTTTTATTCAGTACATCTTCCCGAGGCGAGACCCACAACTGTCCTATGTTT TATTTAAATGATAGCTTCCTGTCTGGCACTGATGAATCTAAGTTCACCGAACTGTGATAATCGGAGTTGG CCTGTTAGTAATTTGGAGGTGGTTCGGTGATCTCAAATCAACTCTATTCTATTTCCTTCCTGAACAGCTC TTGACAGATCGCTCAGGGCTGCCTCCCCCTGCCTCATCCTGAGCCAACAAAAGCACAGTGACTTTCCTGA GAGTCCCGTTATTTCTAGGAGGTAGCAGTTGCTTTAATACCTTTCAGGCTGGGTATTTACACAGAATCAC CTGTAGTACTCATTTCCAACACAGAGCTAGAGAATCAACAGAGTTGAGCAACCTCCTCATCTCAGAGTTT GAGGATTACTGGCTTGAATTGTGGGCAAGGGTCCAGGATCTAAAGGTGCTTTAAGGCGTCCTCTGCTGAC ACTGTTGGTCTCTACTGTCCGTCCTCAGCCTCTGCACACACCACATGGAGACCAGAACTCTAAATCCATG GGGATGCAACCCCTGGGGTTGGCCTGCTTTCAGGCGCTCAGTGAGTTGAGAGTTCTCACAGTTAGTTGTT GCTCACCATAGTACGGATGACTTGTTTACATATGGCTTCCCTTGGAAGCCCTCCTTGACTGTAACTGATG TGAGAGACTAGATCAGTGCTTGGGAGAACTTGATAACCAGTGTCACATGCTTGCCATTTTAAAAGCATGT GTCAGTTGTGTGGGTTTAGTTTTTGTTGTTTTTGTTTTTATCTTTACATACGCGCACACACATACACACA CACTTTAATTTTTTAATAAAATTTTTTTCAATAAATGAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	AscI-NotI
ACCN	BC058400
Insert Size	978 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC058400, AAH58400
RefSeq Size	2511 bp
RefSeq ORF	978 bp
Locus ID	20439
Gene Summary	E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:11257006, PubMed:14645235, PubMed:14645526, PubMed:17003045, PubMed:9637679, PubMed:26070566). E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed:11257006, PubMed:14645235, PubMed:14645526, PubMed:17003045, PubMed:9637679, PubMed:26070566). Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Mediates ubiquitination and proteasomal degradation of DYRK2 in response to hypoxia. Promotes monoubiquitination of SNCA (By similarity). Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (GPS2, POU2AF1, PML, NCOR1), a cell surface receptor (DCC), an antiapoptotic protein (BAG1), and a protein involved in synaptic vesicle function in neurons (SYP) (PubMed:11257006, PubMed:14645235, PubMed:14645526, PubMed:17003045, PubMed:9637679, PubMed:26070566). It is thereby involved in apoptosis, tumor suppression, cell cycle, transcription and signaling processes. Has some overlapping function with SIAH1. Triggers the ubiquitin-mediated degradation of TRAF2, whereas SIAH1 does not. Regulates cellular clock function via ubiquitination of the circadian transcriptional repressors NR1D1 and NR1D2 leading to their proteasomal degradation. Plays an important role in mediating the rhythmic degradation/clearance of NR1D1 and NR1D2 contributing to their circadian profile of protein abundance (PubMed:26392558).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.