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Hadha (NM_178878) Mouse Untagged Clone
CAT＃: MC205925
Hadha (untagged) - Mouse hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit (Hadha), nuclear gene encoding mitochondrial protein, (10ug)
CNY 5,600.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | C77020; Mtpa |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC058569
GAAGAGCTTTCGTCCTCTTCTGCTCAAGATGGTGGCGTCCCGGGCGATTGGCAGTCTCAGTCGCTTCTCT GCCTTCAGGATCCTACGCTCCAGAGGCTGCATTTGCCGCAGCTTTACAACGTCTTCTGCTCTGCTGACCA GAACCCATATTAATTATGGCGTCAAAGGGGATGTGGCAGTTATTCGGATTAACTCACCCAATTCAAAGGT AAATACATTGAATAAAGAAGTACAGTCAGAGTTCATAGAAGTCATGAACGAAATCTGGGCCAACGACCAA ATCAGGAGTGCTGTCCTTATCTCATCAAAGCCTGGCTGCTTTGTCGCAGGGGCTGACATCAACATGCTAT CCTCTTGTACAACCCCCCAAGAAGCAACACGAATATCACAGGAAGGTCAGAGAATGTTTGAGAAACTTGA AAAGTCCCCAAAGCCCGTTGTGGCCGCCATCAGTGGATCCTGCTTGGGAGGAGGACTTGAGCTTGCCATA GCATGCCAATACAGAATAGCAACAAAAGACAGAAAAACAGTATTAGGTGTCCCTGAAGTGTTGCTGGGGA TCTTACCAGGAGCCGGAGGTACCCAGAGACTGCCCAAAATGGTGGGTGTGCCTGCTGCATTTGACATGAT GCTGACTGGCAGGAACATTCGTGCAGACAGAGCAAAGAAAATGGGACTGGTTGACCAGTTGGTGGAACCG CTGGGACCAGGAATAAAATCACCAGAAGAAAGGACAATTGAATACCTAGAGGAAGTTGCAGTTAATTTTG CCAAAGGCCTGGCTGATCGGAAAGTCTCTGCAAAGCAGAGCAAAGGCCTTGTGGAAAAGCTGACAACGTA TGCCATGACTGTCCCATTTGTCAGACAACAGGTTTACAAAACAGTTGAAGAAAAAGTGAAGAAGCAGACT AAAGGCCTTTATCCTGCCCCTTTGAAAATAATTGATGCTGTGAAGGCTGGACTTGAGCAAGGCAGTGACG CTGGTTATCTTGCTGAATCGCAGAAATTTGGAGAGCTTGCATTGACCAAAGAATCGAAGGCCCTGATGGG GCTTTATAACGGCCAGGTCCTGTGCAAGAAAAATAAATTTGGAGCACCACAGAAGAACGTTCAGCAGCTA GCCATCCTTGGCGCAGGGCTGATGGGGGCTGGCATTGCCCAGGTCTCTGTGGATAAGGGACTGAAAACTC TTCTTAAAGACACCACAGTGACGGGGCTGGGCCGGGGACAGCAGCAAGTGTTCAAAGGGCTGAACGACAA AGTGAAGAAGAAAGCTCTCACATCATTTGAAAGGGATTCCATCTTCAGCAACTTGATTGGGCAGCTGGAT TACAAAGGCTTTGAGAAGGCCGACATGGTGATTGAGGCTGTGTTTGAGGACCTCGGTGTAAAGCACAAAG TGTTAAAGGAAGTGGAAAGCGTGACTCCAGAACACTGTATCTTTGCCAGCAACACATCTGCTCTCCCAAT CAATCAAATTGCTGCTGTCAGCAAAAGGCCTGAGAAGGTGATCGGCATGCACTACTTCTCCCCCGTGGAC AAGATGCAGCTCCTAGAAATCATCACCACTGACAAAACCTCCAAGGACACAACGGCCTCTGCCGTGGCCG TGGGGCTCAGGCAAGGGAAGGTCATCATTGTGGTCAAGGACGGACCCGGCTTCTACACCACCAGGTGTCT TGCACCCATGATGTCTGAAGTCATGCGAATCCTCCAGGAAGGAGTTGACCCTAAGAAGCTGGACGCCTTG ACCACAGGTTTCGGCTTCCCTGTGGGTGCTGCCACGCTGGCAGACGAAGTGGGTGTGGATGTAGCACAGC ACGTAGCAGAAGATCTAGGCAAAGCCTTTGGGGAGCGATTTGGAGGTGGCAGCGTAGAACTGCTGAAACA GATGGTCTCCAAGGGCTTCTTAGGTCGCAAGTCTGGGAAGGGCTTTTACATCTATCAGGAGGGCTCAAAG AATAAGAGTTTGAATTCTGAAATGGATAATATCTTGGCAAATCTGAGGCTGCCTGCCAAGCCCGAGGTCT CCTCTGATGAAGACGTCCAATACCGTGTGATAACAAGGTTCGTGAACGAGGCAGTCCTGTGCCTACAGGA AGGGATCCTGGCCACACCGGCAGAGGGAGACATCGGAGCTGTCTTTGGGCTTGGCTTTCCCCCTTGTCTC GGAGGGCCCTTCCGCTTTGTGGATCTGTATGGTGCTCAGAAGGTAGTGGACCGGCTACGGAAGTACGAGT CTGCCTATGGAACACAGTTTACCCCATGCCAGCTGCTCCTCGACCACGCTAACAACTCTAGCAAGAAGTT CTACCAGTGACAGGCCCTCCCACCCTACCCCACCATGCACTAACCCAGACCTGGCAGTGCCGCTTCTCAG CTGTGCTGTCCAAATTATCAGGAAGCAGGAAAAAGACCAAGGCTAGCCTTGGGCTTGCTCCTCCATCAGA GTGCCTTCTGCCCTGGCCCGCTCTTCCTCCTGGTGAAGTCTGACTGTGAATTAACTGTTTGCACTTCATG TTAAGGGGGAGCCCCACTGTGCTTCTTTTGCAAGCCCTGCCTGAGACCCAAATCAACAGCTAAGAGTAAC CCAGAACACCTGCTGCCTGTGCCTTCCCAGAGGCCAGTGGGGCCTGGCAGTGGTAAGGGCATTTTTTGCA CCAAGCCAAACACAGGATAACAATAAAATCCGGACTTTGTGCCTCAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_178878 |
| Insert Size | 2292 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC058569, AAH58569 |
| RefSeq Size | 2749 bp |
| RefSeq ORF | 2292 bp |
| Locus ID | 97212 |
| UniProt ID | Q8BMS1 |
| Gene Summary | Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway. The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA. Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids. Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA described here carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities while the trifunctional enzyme subunit beta/HADHB bears the 3-ketoacyl-CoA thiolase activity. Independently of the subunit beta, the trifunctional enzyme subunit alpha/HADHA also has a monolysocardiolipin acyltransferase activity. It acylates monolysocardiolipin into cardiolipin, a major mitochondrial membrane phospholipid which plays a key role in apoptosis and supports mitochondrial respiratory chain complexes in the generation of ATP. Allows the acylation of monolysocardiolipin with different acyl-CoA substrates including oleoyl-CoA for which it displays the highest activity.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210526 | Hadha (tGFP-tagged) - Mouse hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A |
CNY 8,360.00 |
|
| MR210526 | Hadha (Myc-DDK-tagged) - Mouse hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit (Hadha), nuclear gene enc |
CNY 5,600.00 |
|
| MR210526L3 | Lenti ORF clone of Hadha (Myc-DDK-tagged) - Mouse hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit (Hadha), nuclear gene enc |
CNY 9,500.00 |
|
| MR210526L4 | Lenti ORF clone of Hadha (mGFP-tagged) - Mouse hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit (Hadha), nuclear gene enc |
CNY 9,500.00 |
