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Nfya (NM_010913) Mouse Untagged Clone
CAT＃: MC205791
Nfya (untagged) - Mouse nuclear transcription factor-Y alpha (Nfya), transcript variant 2, (10ug)
CNY 3,600.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AA407810; Cbf-b; SEZ-10; SEZ10 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC029695
GGCGGCTGGAGCCTCTGATTGGGTTTCGGGGTCCGGTACTGGAGCCAATCAGCGCGGGCAGCGAACCGGG GGAGCGAGGCACGGAGTCCCTACCTGGCAGCTGCCTGGGATCTGTAGAGTGAAGCTTCAGGACTCTTAAC GTGGCCGGGCCATGGAGCAGTATACGACAAACAGCAATAGTTCCACAGAGCAGATCGTGGTGCAGGCCGG CCAGATTCAGCAGCAGGTCCAGGGGCAGCCGTTAATGGTGCAAGTCAGTGGAGGCCAGCTTATTACATCA ACAGGCCAACCCATCATGGTACAGGCTGTGCCTGGTGGACAAGGCCAAACCATCATGCAAGTACCTGTGT CTGGAACACAAGGTTTACAGCAGATACAGTTGGTGCCTCCTGGACAGATCCAGATTCAGGGTGGGCAGGC TGTGCAGGTGCAAGGCCAGCAGGGTCAAACCCAGCAGATCATCATCCAACAGCCACAGACTGCAGTCACT GCTGGCCAGACTCAGACACAACAACAGATTGCTGTCCAGGGACAGCAAGTGGCCCAGACTGCTGAAGGAC AGACTATTGTCTACCAACCAGTTAATGCAGATGGCACAATTCTCCAGCAAGTTACAGTCCCTGTTTCAGG CATGATCACCATCCCAGCAGCCAGTTTGGCAGGGGCACAGATCGTTCAGACAGGAGCCAATACCAACACA ACCAGCAGTGGACAAGGGACGGTCACTGTGACACTGCCTGTGGCAGGGAATGTGGTCAACTCAGGAGGAA TGGTCATGATGGTACCAGGAGCTGGCTCTGTGCCTGCTATCCAAAGAATCCCTTTACCTGGAGCAGAGAT GCTGGAAGAAGAGCCCCTGTATGTGAATGCCAAACAGTATCACCGCATCCTTAAGAGGAGACAAGCACGG GCTAAGCTAGAGGTAGAAGGGAAAATCCCAAAGGAACGAAGGAAATACCTCCATGAGTCTCGGCATCGGC ACGCCATGGCACGGAAGCGTGGGGAAGGGGGCCGCTTCTTCTCTCCAAAAGAAAAGGACAGTCCTCACAT GCAGGATCCAAACCAAGCTGACGAAGAAGCCATGACACAGATCATCCGAGTTTCCTAACCACAGGAGGGT GGCAGAGCTGATCAGTCACGGTCCTCTCCACTGTTCTGGAAATGGACTGACTTCCAGTGGGGCACTGACA GTCTCACTCTGCCCTTTCTCAGGACAGAAACTACTTAGCTCAGTATTACAGCTGCAATGATGGCTGACAA ACTGAAGTGGCAAGCTTTTGTCGCACCCCTTCATTGAGGATCTGTTTCAGACTGTTGGACACTGACCTTT TGCAAATTTGGATGATTAAAAAGAGGTATTTGCCAGCCCAGCATTACAAACACAGCACTTATGTTGAGTG GTAAAGCCATCCAGTCAGCCATTGCAGTAGGGGAGAGCAGCCTTCTCAACCTGGTCCTCAGTCGAGGCAG CCACAGCCCCTGCCTCCCTGTGGGTTCTCAACAGCATCCATGCCCTGGTGGGGTGCCGGGCTGCCTGATG CATAGACACTTGGCACTCAGGTGGCTCCCAAGCAAAGAAGCTTCCTGTTAGGACTTGGAGCAACAGACCA AGCTGCTGTCTTCTGACCTGTGCTGATAAGAATCTGTCTGCCCACATGCTAGGTTGGTGTTTACTTCAGC CAAGAAAGTACAAAAAGGATATTTGGACTCAGGATAAAAGGATAGATGGACCTAAAAGGTACATTTCTAG CTTTGACTGTGGTCTGCTCATTTCTATTAACAGCCATCTGGGTCATAGTTCATACTGTGGCCACTTTAGA GACAGTTTAGAGAGTAAAGAACAAAACTTCAAAACATCTGTGCTGAAGTGTTGAGGACATTCACAGTCGT TCTCCAACAGAGGCGAACCTAGGGTATTCAATCATGGGCTTTATGACAGCAAGTAGCAAATGGAAGGATC TGTGCATTTCAATCTAGAGAGCAAGGGCCCTGCTGGCCCTAGTGCTGCTGTAGAGCGTGGTTCTCCAGTG GATGGATAGATACCAGGCATGACTCTTAGGCTGAAGAAACCAAGTCACATTTACATTGACACCTTCTGGC GTGCACTAAAAGAGAATCCTTATATTGCCTGAAGAAGCACTGTTGAGCTAGCTGTCTTGGCTGCAGGCTG GAGCACTGCTGGAGCACAGAGTGGAGGAGAGAGATGTGCTTCATCCCTGCCTGGGTGCTGACCCAGAGGG TGAATGCTCTTCCCATGGCAGCTGATTCTCTGGTGCCAGTTTCCACTTGTTTTCTCCTTTCTCTGTAGGC CTTGCAGCAAGGTTTGCTCACTGCATCAGTGCTGTAGGGACCAACTACAGCCTGCAAGGATTCCTGCAGG GTCAGGAATTGGGCCTCAGAAATGTGTTTTCTGGACAAGCACTCTACCACTGAGCCACACACACATACAT ACACACACACACACACACACACACACCCTTCCCCTGCCCCAGCCCCAGCCCCAACCCCAGCCCCAGCCAA TGCTGAGGTCTGGAAGAAAAGACCAATGTTGATACATCAAAAGAGTTGGCTGCCTTCTCTGCAATCAAAG TCAAACGCAGTCAATCCAAAGACTAAAATACCCGTCAAGTCTAGAGACTGGCCCTTTGACTTTGTTCTGT GTTAAAGATAGGAAAGACTTTAACCTTATAAAGAAATATCGCAGGGAGACCAGTTTAATTGGAGAACCAT GAGGTAAGCCATCTCTTCCTGCTCCCAGGAATCTTGCCATTTCTCCTGGCTTCCGGAGGTCAGAGGGGGC TGATCCTTTTTTTTATAAACTCACTACAAACTTCTGGCTCTTCACATGAGCTTCCGTGTGTTACTGAAAG CCGGGAGGAATTTCTCCCTTTGAGGTGGACAGGATTCTAAGGGGAGGCTTCAGGGATTCCTCCGACTTAA CAGTAGCGCTTCAGCCAGCAGTTGTAGAATGCTATTTATTCGTGTCTGTAAGTTTGTACATAACCCTAAC CTCGTAGGCAAGTTTGAGTTTTCTTTCCTTTGATGCAGATGCTTGGGTTTCCACTCTGGCTGCATTGCAG TCGGCTCCCAGCCCTGCCGGCGTGGGCACAGAGGCCAGAGGGGCTCACTTGGATTTATTTTTGTTTGTTT GTTTATTTGTTTAACTTAGTTCTTTTTACTTGTTCTCGTGATTGTAGTGATGGCTTCAAAGACACAGAGT AAGTGGAGGAGTGGGCTGCTGGTCTACTGGGCACTCTACTTCCTGTTGCAGGATAACTGTAAATGTATAT ATTTTAATTGCACTGGTCTAGACCACTGCACCAACAGCCTCTCCTGGCTACAGGGTGCCTGATAATAATG TCTTGATTTTCCTTTGGGACTCAACCATGTACGTCTCTCTTCACACCCCTCACCCCTCAGCCCCTTCCCC CAGTAGGGGAGTACAGGTGATCACTGATGTCATTGCAATTGCTTTTCTTTTTTAATAAATTTATAAAAAT ACTAGAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_010913 |
| Insert Size | 957 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC029695, AAH29695 |
| RefSeq Size | 3524 bp |
| RefSeq ORF | 957 bp |
| Locus ID | 18044 |
| UniProt ID | P23708 |
| Gene Summary | Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. NF-YA positively regulates the transcription of the core clock component ARNTL/BMAL1.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the coding region, compared to variant 1, resulting in a longer protein (isoform b). |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG204581 | Nfya (tGFP-tagged) - Mouse nuclear transcription factor-Y alpha (Nfya) |
CNY 5,200.00 |
|
| MR204581 | Nfya (Myc-DDK-tagged) - Mouse nuclear transcription factor-Y alpha (Nfya), transcript variant 2 |
CNY 3,600.00 |
|
| MR204581L3 | Lenti ORF clone of Nfya (Myc-DDK-tagged) - Mouse nuclear transcription factor-Y alpha (Nfya), transcript variant 2 |
CNY 5,890.00 |
|
| MR204581L4 | Lenti ORF clone of Nfya (mGFP-tagged) - Mouse nuclear transcription factor-Y alpha (Nfya), transcript variant 2 |
CNY 5,890.00 |
