Kras (NM_021284) Mouse Untagged Clone

CAT#: MC205663

Kras (untagged) - Mouse v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (Kras), (10ug)



  "NM_021284" in other vectors (6)

CNY 2,400.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AI929937; c-K-ras; c-Ki-ras; K-Ras; K-ras; Ki-ras; Kras-2; Kras2; p21B; ras
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC004642
CGGACGCGTGGGCGGCAGCGCTGTGGCGGCGGCTGAGACGGCAGGGGAAGGCGGCGGCGGCTCGGCCCGG AGTCCCGCTCCCGCGCCATTTCGGACCCGGAGCGAGCGCGGCGCGGGCCTGAAGGCGGCGGCGGGAGCCT GAGGCGCGGCGGCTCCGCGGCGCGGAGAGAGGCCTGCTGAAAATGACTGAGTATAAACTTGTGGTGGTTG GAGCTGGTGGCGTAGGCAAGAGCGCCTTGACGATACAGCTAATTCAGAATCACTTTGTGGATGAGTACGA CCCTACGATAGAGGACTCCTACAGGAAACAAGTAGTAATTGATGGAGAAACCTGTCTCTTGGATATTCTC GACACAGCAGGTCAAGAGGAGTACAGTGCAATGAGGGACCAGTACATGAGAACTGGGGAGGGCTTTCTTT GTGTATTTGCCATAAATAATACTAAATCATTTGAAGATATTCACCATTATAGAGAACAAATTAAAAGAGT AAAGGACTCTGAAGATGTGCCTATGGTCCTGGTAGGGAATAAGTGTGATTTGCCTTCTAGAACAGTAGAC ACGAAACAGGCTCAGGAGTTAGCAAGGAGTTACGGGATTCCGTTCATTGAGACCTCAGCAAAGACAAGAC AGGGTGTTGACGATGCCTTCTATACATTAGTCCGAGAAATTCGAAAACATAAAGAAAAGATGAGCAAAGA TGGGAAGAAGAAGAAGAAGAAGTCAAGGACAAGGTGTACAGTTATGTGAATACTTTGTACTCTTTCTTAA GGCACACTTAAGTAAAAGTGTGATTTTTGTACATTACACTAAATTATTAGCATTTGTTTTAGCATTACCT AATCTTTTTTTTTTCTTCTGTTCGTGCAAACTGTCAGCTTTTATCTCAAATGCTTATTTTAAAAGAACAG TGGAAACCTTCTTTTTTCTAAGTGCCAGTATTCCCTGGGTTTTGGACTTAAACTAGCAATGCCTGTGGAA GAGACTAAAGACCTGAGACTCTGTCTTGGGATTTGGTGCATGCAGTTGATTCCTTGCTAGTTCTCTTACC AACTGTGAACACTGATGGGAAGCAGGATAATGAAGCTTCCGGACCATCCCTGCTCTGTGTCCATCTACTC ATCCAATGGAGTCATTAGCAGTCAATCGCAGCTTCACTGGACACTGAGGGGTCACAGACTTAGGCTCCCT TTGAGTCACGTCCAGCGTGTCCTAGACTTTATCATCTTTCAGAGGCGTAGGCAGACTGTTCACAAAGGCT TTCTCTAGCTTTCCACTGCAATTAATCTTGGTCACTCCCTCAAATAGTATATTTTTTCTAGAAAAGGGGA AAAATGGAAAAAAAAAAAAAGGCAATGGAAAATGTTGAAATCCATTCAGTTTCCATGTTAGCTAAATTAC TGTAAGATTCCTATAATAGCTTTTCCTGGTAAGGCAGACCCAGTATGAAATAGTAATAACCATTTGGGCT ATATTTACATGCTACTAAATTTTTGTAATAATTCAAACAACTTTAGCATATATAAAAAGTTCTCATAAGA ATTAAGTACAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN NM_021284
Insert Size 567 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC004642, AAH04642
RefSeq Size 1567 bp
RefSeq ORF 567 bp
Locus ID 16653
UniProt ID P32883
Gene Summary Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (By similarity). Plays an important role in the regulation of cell proliferation (PubMed:6474169, PubMed:1352876). Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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