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Dcun1d1 (NM_033623) Mouse Untagged Clone
CAT＃: MC204889
Dcun1d1 (untagged) - Mouse DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae) (Dcun1d1), transcript variant 3, (10ug)
CNY 2,400.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | pTes3; Rp42; SCCRO; Tes3 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC037431
CCACGCGTCCGGGGAGAGGTCCGGAGGACACCAACATGAACAAGTTGAAATCATCGCAGAAGGATAAAGT TCGTCAGTTTATGATCTTCACACAATCTAGTGAGAAAACTGCAGTAAGTTGTCTTTCTCAAAATGACTGG AAGTTAGATGTTGCAACAGATAATTTTTTTCAAAATCCAGAACTTTATATACGGGAGAGTGTAAAAGGAT CGTTGGACAGGAAGAAGTTAGAGCAACTGTACACTAGATACAAAGACCCTCAGGATGAAAATAAAATTGG AATAGATGGTATACAGCAGTTCTGTGATGATCTGGCCCTCGATCCAGCCAGCATCAGTGTGTTGATCATT GCATGGAAGTTCAGGGCGGCCACACAGTGCGAGTTCTCCAAACAGGAGTTCATGGATGGCATGACAGAGT TAGGATGTGACAGCATAGAAAAACTAAAGGCCCAAATACCCAAGATGGAACAAGAATTGAAAGAACCAGG ACGATTTAAGGATTTTTACCAGTTTACTTTTAATTTTGCAAAGAATCCAGGACAAAAAGGATTAGATCTA GAAATGGCCATTGCTTACTGGAACTTAGTGCTTAATGGAAGATTTAAATTCTTAGACTTATGGAATAAAT TTTTGTTGGAGCATCATAAACGATCAATACCAAAAGACACGTGGAATCTTCTGTTAGACTTCAGTTCAAT GATTGCAGATGACATGTCCAATTATGATGAAGAAGGAGCATGGCCTGTCCTTATTGATGACTTTGTGGAA TTTGCACGCCCTCAAATTGCTGGGACAAAAAGTACAACAGTGTAGCACTAAAGGAACCTTCTAGAATGTA CATAGTCTGTACAATAAATACAACGGAAAATGCACAGTCAATTTCTGCTGGCTGGACTGAAGATCAATCC TCATAATTCAAACTGAGGGTTGAGACAAAACTTTTAAGGATACATCTTGGACCATATCGTACTCCATTCT TCTACTGGTGGTTTGGGCTTGTCTTCTAGTCTGGGCCACTCTAAGCATTTATAATTCCGACATTGTGGAT TTCATCTTACACCTGTGGACCATCTTAGTCTGTCTTCCCATAAGTCATAGAAGCTTGATGGTGATTATTT TGAGGTTTCCATTCTTGCATAAAGCACAATGCTGTCTTCATCAGAAAACAGTTTGGCATAAGAATTAAAC ATAATAATGAACACCACAACAATTTATAAAAACTTCTTAAGTATATGCTTTGGGCTAGTTGCAAAGACTG TGCTGGCAGCACTTCAAATGAGAGTGATTTATCTGAGTGTACCGCATCTGAAATGGTGTTTGGTTGGGGG AATTTAATTTTCCTGGCAAACTACATGTACTGCTGGTGTGAGTAGAATACCTGATATAAGAAATGTCAAA ATAACCAACACATGAAAAAAAAAAGGATAATCTGGGGACTCCCTGAAATATGTCTTGTGTTTCAGACGCT CAGAAAGGTTCTACAGAACAGGTCTGTGGTTATCTTACCCATGTTTATACAGTGTCCTGCAGTGAACTTT TATTTAAAAAAATATTGTAATGAGATCAGATGTTAGACTCCACACTCGTGCATCACACACCACATAATCG GATTTCATTGTGCTCTGAAATACTTCTATGCCTTCTGAATAAGTTAAACTATTTCATTTGTTTTGAATGT TTTGGATTGCTATACAATATAATATTCTAAATTTAGGCATGAGGGTATTAGGTTTGTTTTTTTTTTTTTT AATTTTTTGTCATCGCACTATGGAACACAATGCAATTCACTTAATTTATAAGAAGATAGTAAGATTTAGA TTTTGAAAATGGTTGTGATGAGCCGTGAAGTTCAACCTTTCTCCCATGGCACTGCTCTTTGAGGCGTGGT CCAGCCATGTTGGCTGCTGACTGTTGAGATGGCTTACTGCTAAATCACTGTGGTTGCCAAGTCTACTCCA GAAGCAGAGATTGTCAGACAGGCGAGCACTGGTGCAGATACCAATGGCTTCTGTTCTATAAATTATTTGA CTTTTGACTGTTTTTGTCTCACTTGCATTTGCTATTTCCACATCACAGCAGAATGCAGTCTTGATTGGTT TTGTTTTTTGTTTTTTGTTTTTCCATGATAGGACTCATGCCTTGCTCTCCTGCTGAAAATGCTTGTTTCT CTGATAACATACTGACATGTGAAAGGGGTGTATTCATACATATTTCCATGAGGAAGTTAAAATGATGAAC TAAATGTTGGGTGACTGGTCTTCAATAGGAACAGATTTTGCTTCATGTATCCAGGTCCTCTGCAGGGTAC CAAGGAATGTTCTTCAGGATAGCGGAACAATTTATGCAGATATGGAAATGATTAAAACTGCTTTTTAGTC ACATGATTTGGAAAATGCATTGAAAGCTTACCTTACAGATATATCATTAATCCAATCATAACTTTAATTT AGGAATGCATTTCTACCAAAAGAAGCTTAGAGTAAATTTCTATTCATGTTACAGGGATTGGTCATTAAAA GGAAAAGAATCTTGCTGTTTTCAGTATTTCTTGATTTCATTTTTTGTAAATATAAAGAACTTCAATTATG AAAAAAATTAAAGATATATGTATATATATATCTTTAAAAGTTTTACAGACCTATATTGTTTATTCCCTAC ATGGAACATTTTGAGTTTTGGTATTGTTTTCAGATTGATTGATTCAGATATGATTTGTTTTCAAATGAGA CTCCATTAGCTTCTTTCCCTGCCTCATAAATTTTTTTTTCATAAAAATCTTGATATTTGTGTGTGGCCTA ATGCTTTGGGTTTTACAGATTTTATTTTTAAATGCAAGACTTTTTAACAAAATAGTGTTTGTCATCAGTT TGGTACTAAACATTTACAATTACTGTGTAATTATAAACAAAAATATATAAAGCTTTTATTATGTAGCAAA AAGAGTTAAGGTTGTTCACCATGATGGCATCTTAGAATTAAACAAGACTTGCTAGGGCTCCAAGAGAAAA TTGATTTAATGTGTTTATTCTGAGGGTGACTGTCTGGTTCTAGGAAATGTTTTGTAATACAAATGCTTAC CCTGTTGGGGGCGGGGCGTCGGGGAGTCTTCCAGAGGAAGGGCACACCCACTCACACACAGCCAGCTATT GCCGGCAGAGGATCATGGGGTTCAGTCTTCGTGTGGGTCAGGTCTCCAAGCTGATTCTTTTCTTTCCAGA CAGAACCATAGGGAGAGTTTACTTTTTGCCAAATGTCAGAACAGGTACACATTTTTAAATGTAATGCTTT TTAAATAGTAAACTGTATAAAATGAGAAGTACCCACATATAAAAATACTTGAGGTCAAGGTTCTCTTGAG TGACTATCACCTGCATCTCTCTGTACGTTCAGGTGTGTCGTTTGCAATCCCTGTCATATTACCCACGGAG GCAATAAAAGCTTCAGTAAATTTGCCATGAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_033623 |
| Insert Size | 780 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC037431, AAH37431 |
| RefSeq Size | 3478 bp |
| RefSeq ORF | 780 bp |
| Locus ID | 114893 |
| Gene Summary | Part of an E3 ubiquitin ligase complex for neddylation. Promotes neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes. Acts by binding to cullin-RBX1 complexes in the cytoplasm and promoting their nuclear translocation, enhancing recruitment of E2-NEDD8 (UBE2M-NEDD8) thioester to the complex, and optimizing the orientation of proteins in the complex to allow efficient transfer of NEDD8 from the E2 to the cullin substrates. Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity (By similarity). Acts also as an oncogene facilitating malignant transformation and carcinogenic in vivo (PubMed:20563250).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region and uses a downstream, in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG203034 | Dcun1d1 (tGFP-tagged) - Mouse DCUN1D1 DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae) (Dcun1d1) |
CNY 2,850.00 |
|
| MR203034 | Dcun1d1 (Myc-DDK-tagged) - Mouse DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae) (Dcun1d1), transcript variant 3 |
CNY 2,400.00 |
|
| MR203034L3 | Lenti ORF clone of Dcun1d1 (Myc-DDK-tagged) - Mouse DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae) (Dcun1d1), transcript variant 3 |
CNY 4,750.00 |
|
| MR203034L4 | Lenti ORF clone of Dcun1d1 (mGFP-tagged) - Mouse DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae) (Dcun1d1), transcript variant 3 |
CNY 4,750.00 |
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