Lin28a (NM_145833) Mouse Untagged Clone

CAT#: MC204860

Lin28a (untagged) - Mouse lin-28 homolog A (Lin28a), (10ug)



  "NM_145833" in other vectors (4)

CNY 2,400.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AL024421; ENSMUSG00000070700; Gm10299; Lin-28; lin-28A; Lin28; Tex17
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC068304
GGGGCCCGGGGCCACGGGCTCAGCAGACGACCATGGGCTCGGTGTCCAACCAGCAGTTTGCAGGTGGCTG CGCCAAGGCAGCGGAGAAGGCGCCAGAGGAGGCGCCGCCTGACGCGGCCCGAGCGGCAGACGAGCCGCAG CTGCTGCACGGGGCCGGCATCTGTAAGTGGTTCAACGTGCGCATGGGGTTCGGCTTCCTGTCTATGACCG CCCGCGCTGGGGTCGCGCTCGACCCCCCGGTGGACGTCTTTGTGCACCAGAGCAAGCTGCACATGGAAGG GTTCCGAAGCCTCAAGGAGGGTGAGGCGGTGGAGTTCACCTTTAAGAAGTCTGCCAAGGGTCTGGAATCC ATCCGTGTCACTGGCCCTGGTGGTGTGTTCTGTATTGGGAGTGAGCGGCGGCCAAAAGGGAAGAACATGC AGAAGCGAAGATCCAAAGGAGACAGGTGCTACAACTGCGGTGGGCTAGACCATCATGCCAAGGAATGCAA GCTGCCACCCCAGCCCAAGAAGTGCCACTTTTGCCAAAGCATCAACCATATGGTGGCCTCGTGTCCACTG AAGGCCCAGCAGGGCCCCAGTTCTCAGGGAAAGCCTGCCTACTTCCGGGAGGATGAGGAAGAGATCCACA GCCCTGCCCTGCTCCCAGAAGCCCAGAATTGAGGCCCAGGAGTCAGGGTTATTCTTTGGCTAATGGGGAG TTTAAGGAAAGAGGCATCAATCTGCAGAGTGGAGAAAGTGGGGGTAAGGGTGGGTTGCGTGGGTAGCTTG CACTGCCGTGTCTCAGGCCGGGGTTCCCAGTGTCACCCTGTCTTTCCTTGGAGGGAAGGAAAGGATGAGA CAAAGGAACTCCTACCACACTCTATCTGAAAGCAAGTGAAGGCTTTTGTGGGGAGGAACCACCCTAGAAC CCGAGGCTTTGCCAAGTGGCTGGGCTAGGGAAGTTCTTTTGTAGAAGGCTGTGTGATATTTCCCTTGCCA GACGGGAAGCGAAACAAGTGTCAAACCAAGATTACTGAACCTACCCCTCCAGCTACTATGTTCTGGGGAA GGGACTCCCAGGAGCAGGGCGAGGTTATTTTCACACCGTGCTTATTCATAACCCTGTCCTTTGGTGCTGT GCTGGGAATGGTCTCTAGCAACGGGTTGTGATGACAGGCAAAGAGGGTGGTTGGGGAGACAACTGCTGAC CTGCTGCCCACACCTCACTCCCAGCCCTTTCTGGGCCAATGGGATTTTAATTTATTTGCTCCCTTAGGTA ACTGCACCTTGGGTCCCACTTTCTCCAGGATGCCAACTGCACTATCTACGTGCGAATGACGTATCTTGTG CGTTTTTTTTTTTTTTAATTTTTAAAATTTTTTTTCATCTTCTTAATATAAATAATGGGTTTGTATTTTT GTATATTTTAATCTTAAGGCCCTCATTCCTGCACTGTGTTCTCAGGTACATGAGCAATCTCAGGGATAAT AAGTCCGTAGCAGCTCCAGGTCTGCTCAGCAGGAATACTTTGTTTTGTTTTGTTTTGATCACCATGGAGA CCAACCATTTGGAGTGCACAGCCTGTTGAACTACCTCATTTTTGCCGATTACAGCTGGCTTTTCTGCCAT AGCGTCCTTGAAAAATGTGTCTCACGGGTTTCGATTGAGCTGCCCCAAGACTTGATCTGGATTTGGCAAA ACATAGGACATCACTCTAAACAGGAAAGGGTGGTACAGAGACATTAAAAGGCTGGGCCAGGTGAAAGGCA CAAGAGGAACTTTCCATACCAGATCCATCCTTTTGCCAGATTAGTGGAAGCCTGCCATGCACAGCAGGGT GTGAGAGAGAGAGTGTGTATGTATGTGTGTGTGGATTTTTTTTTAATGCAAATTTATGAAGACGAGGTGG GTTTTGTTTATTTGATTGCTTTTTGTGCTGGGGATGGAATCTTGGGCTTCATTTGTGCTAGGAAGTACAC TGCCACTGAGTTATCCCAGTAAGAATGCAACTTAAGACCAGTACCCTTATTCCCACACTGTGCTGTCCAG GCATGGGAACATGAGGCAGGGACTCAACTCCTTAGCCTTTCACAATCTTGGCTTTCTGAGAGACTCATGA GTATGGGCCTCAGTGGCAAGTGTCCTGCCCTGCTGTAACGTGATGGTTGATAGCTAAAGGAAAGAGGGGG TGGGGAGTTTCGTTTACATGCTTTGAGATCGCCACAAACCTACCTCACTGTGTTGAAACGGGACAAATGC AATAGAACACATTGGGTGGTGTGTGTGTGTGTCTGATCTTGGTTTCTTGTCTCCCTCTCCCCCCAAATGC TGCCCTCACCCCTAGTTAATTGTATTCGTCTGGCCTTTGTAGGACTTTTACTGTCTCTGAGTTGGTGATT GCTAGGTGGCCTAGTTGTGTAAATATAAATGTGTTGGTCTTCATGTTCTTTTGGGGTTTTATTGTTTACA AAACTTTTGTTGTATTGAGAGAAAAATAGCCAAAGCATCTTTGACAGAAAGCTCTGCACCAGACAACACC ATCTGAAACTTAAATGTGCGGTCCTCTTCTCAAAGTGAACCTCTGGGACCATGGCTTATCCTTACCTGTT CCTCCTGTGTCTCCCATTCTGGACCACAGTGACCTTCAGACAGCCCCTCTTCTCCCTCGTAAGAAAACTT AGGCTCATTTACTTCTTTGAGCATCTCTGTAACTCTTGAAGGACCCATGTGAAAATTCTGAAGAAGCCAG GAACCTCATTCTTTCCTTGTCCCTAACTCAGTGAAGAGTTTTGGTTGGTGGTTTTGAGACAGGGCCTCAT TCTGTAGCTGGAGATAGAGAGCCTCGGGTTCCTGGCTCTCCTCCTGCCTTCTGCACAGAGTCCCCTGTGC AGGGATTGCAGGTGCCGCTTCTCCCTGGCAAGACCATTTATTTCATGGTGTGATTCGCCTTTGGATGGAT CAAACCAATGTAATCTGTCACCCTTAGGTCGAGAGAAGCAATTGTGGGGCCTTCCATGTAGAAAGTTGGA ATCTGGACACCAGAAAAGGGACTATGAATGTACAGTGAGTCACTCAGGAACTTAATGCCGGTGCAAGAAA CTTATGTCAAAGAGGCCACAAGATTGTTACTAGGAGACGGACGAATGTATCTCCATGTTTACTGCTAGAA ACCAAAGCTTTGTGAGAAATCTTGAATTTATGGGGAGGGTGGGAAAGGGTGTACTTGTCTGTCCTTTCCC CATCTCTTTCCTGAACTGCAGGAGACTAAGGCCCCCCACCCCCCGGGGCTTGGATGACCCCCACCCCTGC CTGGGGTGTTTTATTTCCTAGTTGATTTTTACTGTACCCGGGCCCTTGTATTCCTATCGTATAATCATCC TGTGACACATGCTGACTTTTCCTTCCCTTCTCTTCCCTGGGAAAATAAAGACTTATTGGTACTCCAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAA
Restriction Sites RsrII-NotI     
ACCN NM_145833
Insert Size 630 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC068304, AAH68304
RefSeq Size 3505 bp
RefSeq ORF 630 bp
Locus ID 83557
UniProt ID Q8K3Y3
Gene Summary RNA-binding protein that inhibits processing of pre-let-7 miRNAs and regulates translation of mRNAs that control developmental timing, pluripotency and metabolism (PubMed:17473174, PubMed:18604195, PubMed:18566191, PubMed:18292307, PubMed:19703396, PubMed:23102813, PubMed:24209617). Seems to recognize a common structural G-quartet (G4) feature in its miRNA and mRNA targets (PubMed:26045559). 'Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in mRNA stabilization. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (PubMed:17473174). Suppressor of microRNA (miRNA) biogenesis, including that of let-7, miR107, miR-143 and miR-200c. Specifically binds the miRNA precursors (pre-miRNAs), recognizing an 5'-GGAG-3' motif found in pre-miRNA terminal loop, and recruits TUT4 and TUT7 uridylyltransferaseS. This results in the terminal uridylation of target pre-miRNAs. Uridylated pre-miRNAs fail to be processed by Dicer and undergo degradation. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state by preventing let-7-mediated differentiation of embryonic stem cells (PubMed:19703396, PubMed:28671666). Localized to the periendoplasmic reticulum area, binds to a large number of spliced mRNAs and inhibits the translation of mRNAs destined for the ER, reducing the synthesis of transmembrane proteins, ER or Golgi lumen proteins, and secretory proteins (PubMed:23102813). Binds to and enhances the translation of mRNAs for several metabolic enzymes, such as PFKP, PDHA1 or SDHA, increasing glycolysis and oxidative phosphorylation. Which, with the let-7 repression may enhance tissue repair in adult tissue (PubMed:24209617).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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