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Aph1a (NM_146104) Mouse Untagged Clone
CAT＃: MC204622
Aph1a (untagged) - Mouse anterior pharynx defective 1a homolog (C. elegans) (Aph1a), transcript variant 1, (10ug)
CNY 2,400.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 6530402N02Rik; APH-1a |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC057865
CCGTAGACGAGGTCGGGCGGACCTTCGTCCGGGGTTTCGGCACCCCCCCTTCCCCTTCCCCGGGGTCCGG GGGTGACATTGCACCGCGCCCCTCCTGGGGTTGCGTCCTCGCCCCACGCGGACTCCCCCTCGGCGCGCAG CTCCCTTTCGCCTTCCTTGGCCGGGGTTCCCTCCCTTCCCCAGCTGCCCAGTCATGGGGGCTGCTGTGTT TTTCGGATGCACCTTCGTCGCGTTCGGCCCAGCCTTCTCCCTTTTCCTGATCACTGTAGCTGGAGACCCA CTTCGGGTTATCATCCTGGTGGCGGGAGCCTTTTTCTGGCTGGTCTCCCTGCTCTTGGCTTCTGTGGTCT GGTTCATCTTGGTCCATGTGACAGACCGATCAGATGCACGGCTCCAGTATGGCCTCCTGATTTTTGGTGC TGCTGTCTCTGTCCTTCTACAGGAAGTGTTCCGTTTTGCTTACTACAAGCTCCTTAAGAAGGCAGATGAG GGCTTAGCATCACTGAGTGAGGACGGAAGATCACCCATCTCCATCCGACAGATGGCCTATGTTTCTGGTC TGTCCTTCGGTATCATCAGTGGTGTCTTCTCTGTTATCAATATTTTGGCTGATGCACTTGGGCCAGGTGT GGTTGGGATCCATGGAGACTCACCCTATTACTTCCTGACTTCAGCCTTTCTGACAGCAGCCATTATCCTG CTCCACACCTTTTGGGGAGTTGTGTTCTTTGATGCCTGTGAGAGGAGACGGTACTGGGCTTTGGGCCTGG TAGTTGGGAGTCACCTTCTGACATCGGGACTGACATTCCTGAACCCCTGGTATGAGGCTAGCCTGCTGCC CATCTATGCAGTCACCGTTTCCATGGGGCTCTGGGCGTTCATCACAGCCGGAGGCTCCCTCCGAAGTATC CAGCGCAGCCTTTCGTGTAAGGACTGACTACCTGGACTGATCGCCCGACAGATCCCATCTGCCTATCCAC TGCCCATGACTGAACCCAGCCCCAGCCCGGGTCCATTGCCCTCATCCTCCGTCTCCTCGCTGATGTGCCC CGCTTCCTTCCGGGTTTGGCGTTGTCCATTTGTGACCTGTAGTCTCTAAGCTTTCTCAGGAGCAGCCTGG GTGCAGCCAGTCAGGGACTGGTGGGTTTGAATCTGCATCTCTCCCCACCACCTGGGGACCCCCTTGTTGT CCAGGTCTCCCCATGTGTCAGTGCTCCACCCTCACCCTGCCCATGACTCACCCCGCTTCCCCTCTGCAGG CCGCCGGCAGGAGGACAGTCGGGTGATGGTGTACTCTGCCCTGCGCATCCCACCCGAGGACTGAGGGAAC ATGGGGGGGCCCCTGGGCCTGGGGTGCCCTCCCGATCTCCTCGTCCCGTATTTCTCCATCTCCAGTTCTG GACAGTGCAGGTTGCCAAGAAAAGGGACCTAGCTTAGCCATGGCCCTGGAGATGAAGTTACTGAGGCTGA GGGTAGATGGGTTCAGTTCAGTGTCTCCTGCCCAGACTGGACATCTTGGTCTTTTTCTCAGGTCTGAAAG GAACCATTTTTTGGTATGATAAAGACCCCAAAATGCCTTTTTTTTAAAGTGGGGAGAGGGAGGAGGTACG CCGGGAGCCTCTTTAACCTCCATAGGCTGTGTTTGCTCCCAAATCTGCTGATGGTGGCTCATTTCTATCC TGTTTTCCCTTTGGTCCCAGGCCCTGGGGGAAAGGAAGGAAGTGCATGCTTGGGGACTGGTCCCACTGGA TCTCTATCCTAACGTCCATGGCCGTCAGCACCCCTCCTTTCCCCAAGGTGAAATGGAGGGTGCTGTGGGG GCCTGCCCACTCCATGGCAGTGCCCTGAAGGAGTCAGACTACCATGGCATCGCAGGCCCAGAGGGCAGAC ATTTTTTCTAGTTTTTAATTGGGGTGTGGGAGGGTTTGGGGAGGTTTTCTATCACTGTGTTGTTTTTCTG CTGAGGGTGGAATATTCTGTCCTTTTAATCAAGGTGATTGTGATTTTGACTAATAAAAAGGACTTTATAA TTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_146104 |
| Insert Size | 744 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC057865, AAH57865 |
| RefSeq Size | 2104 bp |
| RefSeq ORF | 744 bp |
| Locus ID | 226548 |
| UniProt ID | Q8BVF7 |
| Gene Summary | This gene encodes a subunit of the gamma-secretase complex, which is localized to the endoplasmic reticulum and golgi apparatus. Gamma-secretase is a multi-protein enzyme that catalyzes intramembraneous proteolysis of type I transmembrane proteins and is essential for many signaling pathways, including the Notch signaling pathway. Studies suggest that the protein encoded by this locus binds directly to substrates of the gamma-secretase complex, including the beta-amyloid precursor protein which is associated with Alzheimer disease progression. This gene is required for normal embryonic development and survival, and disruption is associated with defects in the yolk sack angiogenesis, neural tube formation, and somitogenesis. A pseudogene of this gene is located on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013] Transcript Variant: This variant (1) differs in the 3' coding region and 3' UTR, compared to variant 2. The resulting isoform (1, also known as isoform S) is shorter and has a distinct C-terminus, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data.The genomic coordinates used for the transcript record were based on alignments. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG223505 | Aph1a (tGFP-tagged) - Mouse anterior pharynx defective 1a homolog (C. elegans) (Aph1a) transcript variant 1, (10ug) |
CNY 2,850.00 |
|
| MR223505 | Aph1a (Myc-DDK-tagged) - Mouse anterior pharynx defective 1a homolog (C. elegans) (Aph1a), transcript variant 1 |
CNY 2,400.00 |
|
| MR223505L3 | Lenti ORF clone of Aph1a (Myc-DDK-tagged) - Mouse anterior pharynx defective 1a homolog (C. elegans) (Aph1a), transcript variant 1 |
CNY 4,750.00 |
|
| MR223505L4 | Lenti ORF clone of Aph1a (mGFP-tagged) - Mouse anterior pharynx defective 1a homolog (C. elegans) (Aph1a), transcript variant 1 |
CNY 4,750.00 |
