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Bub1 (NM_009772) Mouse Untagged Clone
CAT＃: MC204570
Bub1 (untagged) - Mouse budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae) (Bub1), transcript variant 2, (10ug)
CNY 7,384.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AL022991; Bub1a; C80208; D2Xrf87 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC029116
CGAGACTCGGGGTTAGGGGCTGATGGGGAGTTTGGTCGGCGGCTGGTGGGGTATTTCGCCGCTGCGCGTT TTTTCTCGTCATGGACAACCTAGAAAATGTCTTTCGCATGTTTGAAGCCCATATGCAAAGCTACACGGGT AATGACCCACTTGGAGAATGGGAAAGCTTTATAAAGTGGGTAGAAGAGAATTTTCCTGACAATAAAGAAT ACTTGATGACATTATTAGAACATTTAATGAAGGAATTTTTACATAAGAAGAACTACCACAATGATTCAAG ATTCATCAATTATTGCTTAAAATTTGCTGAGTACAACAGCGACCGTCATCAGTTTTTTGAGTTTCTGTAC AACCAGGGAATTGGAACCAAGTCATCATATATATACATGTCCTGGGCAGGGCATCTGGAAGCCCAGGGAG AGCTGCAGCATGCCAGTGCTATTTTTCAGACAGGAATTCACAATGAGGCTGAACCTAAAGAACTACTACA GCAACAATACAGGCTATTCCAAGCACGCCTTACTGGAATCCATTTGCCAGCTCAAGCTACAACCTCAGAA CCTTTGCATAGTGCACAGATTTTAAACCAAGTTATGATGACAAACTCAAGTCCAGAAAAAAACTCAGCCT GTGTTCCTAAGAGTCAGGGTTCAGAATGTTCTGGTGTGGCATCTTCCACTTGTGATGAAAAGTCTAATAT TAGGGAACAAAGGGTGATCATGATTTCCAAGTCAGAATGCTCTGTCAGCTCATCTGTGGCACCCAAGCCT GAGGCTCAGCAAGTTATGTACTGCAAGGAAAAGCTTATTCGTGGAGATTCAGAATTTTCTTTTGAAGAAC TGAGAGCCCAGAAATATAATCAAAGGAAGAAGCATGAGCAGTGGGTTAGTGAAGACAGAAATTATATGAA AAGGAAAGAAGCAAATGCTTTTGAAGAGCAATTATTAAAACAGAAAATGGATGAACTTCACAAGAAATTG CATCAAGTGGTGGAATTGTCACACAAGGACCTTCCTGCTTCTGAGAACAGGCCTGATGTTAGTCTAGTAT GTGTTGGACAAAATACTTGCTCCCAGCAGGAATTGAGGGGTCCAAGTCTTTCATCCATCAGTCATCAGAC CTCAGAGAGTTCAGGAGAGAAACCACAGGAAGAACCTTCTGTTCCTCTTATGGTAAATGCTGTTAACAGC ACTTTGCTGTTCCCAGCTGCCAACCTGCCAGCTCTTCCTGTTCCTGTAAGTGGCCAGTCATTGACAGACT CCAGATGTGTGAATCAAAGTGTTCATGAATTCATGCCACAGTGTGGACCAGAAACAAAAGAAGTGTGTGA AACAAATAAAGTTGCCAGCATTAATGATTTTCATACAACTCCAAACACATCATTGGGAATGGTTCAAGGA ACACCATGCAAAGTGCAGCCATCACCAACTGTCCACACCAAGGAAGCATTAGGTTTCATCATGGACATGT TTCAGGCTCCAACACTTCCTGACATTTCTGATGATAAAGATGAATGGCCATCTCTGGACCAAAATGAAGA TGCATTTGAAGCCCAGTTTCAAAAAAATGCAGTATCTTCGGGAGATTGGGGAGTTAAAAAAATTATGACT TTGTCATCTGCTTTTCCTATTTTTGAAGATGGAAACAAAGAAAATTATGGCTTACCACAGCCTAAAAATA AGCCCTTAGGAGCTAGGACCTTTGGAGAACGATCTCTCAGTAAATATTCCTCGAGATCAAATGAAATGCC TCACACTGATGAGTTTATGGATGATTCAACAGTATGTGGTATTCGCTGCAACAAAACTCTAGCTCCCAGT CCTAAAAGTATAGGAGACTTTACATCTGCTGCCCAACTTTCGTCTACACCATTCCACAAATTTCCAGCAG ATTTAGTACAGATTCCAGAAGATAAAGAAAATGTGGTAGCCACACAGTATACACATATGGCTTTGGATTC TTGTAAAGAAAACATAGTGGACCTCTCAAAAGGCAGAAAGCTTGGGCCAATTCAAGAGAAAATTTCAGCA TCTTTACCCTGTCCTAGTCAGCCTGCCACAGGTGGTTTGTTCACCCAGGAAGCAGTGTTCGGCCTTGAGG CTTTTAAATGCACAGGCATTGACCATGCGACAGTGGAAGACCTATCCGATGCCAATGCTGGGCTCCAAGT TGAATGCGTGCAGACACTTGGAAATGTCAATGCTCCAAGCTTTACTGTTGAGAACCCATGGGATGATGAA TTGATTCTTAAACTTCTCTCTGGACTTTCTAAGCCAGTTACTTCCTATTCAAATACTTTTGAGTGGCAGA GTAAACTTCCAGCCATCAAGACCAAGACAGAATATCAATTGGGTTCTTTGCTGGTCTATGTGAATCACCT TCTTGGAGAAGGAGCCTTTGCTCAAGTCTTTGAAGCTATTCATGGAGATGTGAGAAATGCCAAAAGTGAA CAGAAATGCATTTTGAAGGTGCAGAGACCTGCCAACTCCTGGGAATTCTACATTGGGATGCAGCTGATGG AAAGACTAAAGCCAGAAGTACATCACATGTTCATCAAGTTTTATTCTGCTCATTTATTCAAGAACGGCAG CATATTAGTAGGGGAACTCTACAGCTATGGGACGTTACTAAATGTCATTAACCTCTATAAAAATACCTCT GAAAAAGTGATGCCCCAGGCTCTTGTCCTCACTTTCGCTATCAGAATGCTTTACATGGTTGAACAAGTCC ACAGCTGCGAAATCATTCATGGAGACATTAAGCCAGATAACTTCATACTAGGACACAGATTTTTGGAACA GGCTGATGAAGACTTAGCTACCGGCTTGGCATTGATTGACCTGGGTCAGAGTATAGATATGAAACTTTTC CCTAAAGGAACTGTATTTACAGGAAAATGTGAAACATCTGGTTTTCAGTGTCCTGAGATGCTCAGTAACA AGCCATGGAACTACCAGATTGATTACTTTGGAGTTGCTGCAACAATATACTGTATGCTCTTTGGCTCTTA CATGAAAGTAAAAAATGAAGGAGGAGTCTGGAAACCTGAAGGTCTTTTTAGAAGGCTTCCTCATTTGGAT ATGTGGGAGGAATTTTTTCACATCATGTTGAATATACCGGATTGTCATAATCTTCCATCTTTGGATTTTC TGAGACAGAATATGAAGAAATTACTTGAACAACAGTATTCCAACAAGATTAAGACCTTGCGTAATAGGCT AATTGTGATGCTTTCAGAATATAAGCGTTCAAGAAAATAAAATGAATATGGACCTTCTCCAAAACACACT TAGTAAATTTAAACTCTCACTTTGAATCCTATTTTTTTCATACTTACTATTTATGTATGTTTAAAAAAAA TAAAGCCCATATGATATACCCAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_009772 |
| Insert Size | 3180 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC029116, AAH29116 |
| RefSeq Size | 3405 bp |
| RefSeq ORF | 3180 bp |
| Locus ID | 12235 |
| UniProt ID | O08901 |
| Gene Summary | Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Required for centromeric enrichment of AUKRB in prometaphase. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis. Essential during early and later stages of embryonic development. Necessary for postimplantation embryogenesis and proliferation of primary embryonic fibroblasts and plays an important role in spermatogenesis and fertility.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the mid-coding region, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211557 | Bub1 (tGFP-tagged) - Mouse budding uninhibited by benzimidazoles 1 homolog (S |
CNY 11,590.00 |
|
| MR211557 | Bub1 (Myc-DDK-tagged) - Mouse budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae) (Bub1), transcript variant 2 |
CNY 10,550.00 |
|
| MR211557L3 | Lenti ORF clone of Bub1 (Myc-DDK-tagged) - Mouse budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae) (Bub1), transcript variant 2 |
CNY 12,450.00 |
|
| MR211557L4 | Lenti ORF clone of Bub1 (mGFP-tagged) - Mouse budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae) (Bub1), transcript variant 2 |
CNY 12,450.00 |
