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Pfkl (NM_008826) Mouse Untagged Clone
CAT＃: MC203910
Pfkl (untagged) - Mouse phosphofructokinase, liver, B-type (Pfkl), (10ug)
CNY 5,720.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AA407869; ATP-PFK; PFK-B; PFK-L |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC020097
CGGACGCGTGGGCGCGGAACGCAGAGCGGCGGGAGAGGAGCTTGGGCTCCTGGTCTCCGCTGCCGCCATG GCTACCGTGGACCTGGAGAAACTGCGGATGTCGGGGGCTGGCAAGGCCATTGGAGTGCTGACCAGCGGCG GTGATGCGCAAGGTATGAATGCTGCTGTCAGGGCTGTGACCCGTATGGGCATATATGTGGGGGCCAAAGT CTTCCTCATCTATGAGGGCTACGAGGGCCTTGTGGAAGGAGGCGAGAACATCAAGCCAGCCAACTGGCTC AGCGTTTCCAATATCATCCAGCTGGGTGGCACCATTATTGGCAGTGCCCGCTGTAAGGCCTTCACTACGA GGGAAGGCCGCCTGGCTGCAGCCTACAATCTGCTCCAACACGGCATCACCAACCTGTGTGTCATCGGTGG CGATGGCAGCCTCACGGGGGCCAACATCTTCCGCAACGAGTGGGGCAGCTTGCTGGAGGAGCTGGTGAAG GAAGGCAAGATCTCAGAGTCCACAGCTCAGAACTACGCACACTTGACCATCGCCGGTCTGGTGGGCTCCA TCGATAATGACTTCTGCGGCACTGACATGACCATTGGCACAGACTCAGCCCTGCACCGCATTATGGAGGT CATTGACGCCATCACTACCACTGCCCAAAGTCACCAGAGGACCTTTGTTTTGGAGGTGATGGGACGGCAC TGCGGGTACCTGGCGCTGGTGTCTGCGCTGGCTTCCGGGGCTGATTGGCTATTCATTCCTGAAGCGCCCC CTGAGGATGGCTGGGAGAACTTCATGTGTGAGAGGCTGGGCGAGACTCGGAGCCGAGGCTCTCGGCTGAA CATCATCATCATCGCAGAGGGCGCCATTGACCGGCATGGAAAGCCTATCTCATCCAGCTACGTGAAGGAT CTGGTGGTTCAGAGGCTGGGCTTCGATACACGAGTGACTGTGCTGGGTCATGTACAGCGAGGAGGGACGC CTTCAGCCTTCGACCGAATCCTGAGTAGCAAGATGGGTATGGAGGCCGTGATGGCGCTGCTAGAGGCCAC GCCTGACACGCCGGCCTGTGTGGTCAGCCTCTCCGGGAACCAGTCTGTGAGGCTGCCTCTCATGGAGTGT GTGCAAGTGACAAAGGACGTGCAGAAGGCCATGGACGAGGAGAGGTTTGACGAGGCCATCCAGCTCCGTG GCAGGAGCTTTGAGAACAACTGGAAAATTTACAAGCTCCTTGCCCACCAGAAGGTGTCTAAAGAGAAGTC CAACTTCTCCCTGGCCATCCTGAATGTGGGGGCTCCAGCTGCTGGCATGAATGCAGCTGTGCGCTCCGCA GTGCGCACCGGTATCTCCGAGGGACACACGGTATACATCGTGCATGATGGCTTTGAGGGTCTGGCCAAGG GTCAGGTGCAAGAAGTGGGCTGGCATGATGTGGCAGGCTGGCTGGGACGTGGTGGCTCGATGCTGGGGAC CAAGAGGACACTGCCCAAGCCCCATCTGGAGGCCATTGTGGAGAATCTCCGCACCTACAACATCCACGCC CTGCTGGTGATTGGTGGCTTTGAGGCCTACGAGGGTGTGCTGCAGCTGGTGGAGGCCCGGGGGCGCTACG AGGAACTCTGCATTGTCATGTGCGTCATCCCAGCCACCATCAGCAACAATGTGCCTGGCACTGACTTCAG CCTGGGCTCAGACACGGCTGTCAACGCTGCAATGGAGAGTTGTGATCGCATCAAGCAGTCAGCCTCAGGG ACAAAGCGGCGTGTGTTCATTGTAGAGACCATGGGGGGCTACTGTGGCTACCTGGCCACTGTGACCGGCA TTGCTGTGGGTGCCGATGCTGCCTACGTCTTTGAGGACCCTTTCAACATCCATGACTTAAAGGCCAATGT GGAGCATATGACAGAGAAGATGAAGACAGACATCCAGAGGGGACTGGTGCTCCGGAACGAGAAGTGTCAC GAACACTACACCACAGAGTTCCTATACAACCTGTACTCCTCAGAAGGCAGGGGCGTGTTTGACTGCAGGA CCAATGTGCTGGGCCACTTACAGCAGGGTGGTGCTCCAACCCCTTTCGACCGGAACTATGGGACCAAACT GGGGGTGAAGGCCATGTTGTGGGTGTCTGAGAAGCTACGTGATGTCTACCGTAAAGGGCGGGTGTTTGCC AATGCTCCAGACTCAGCCTGTGTGATCGGCCTGCGGAAGAAGGTAGTGGCCTTCAGTCCGGTCACAGAAC TCAAGAAAGAGACTGATTTTGAGCACCGCATGCCCCGGGAGCAGTGGTGGCTGAATCTGCGGCTGATGCT GAAGATGCTGGCACACTATCGCATCAGCATGGCAGACTATGTGTCTGGGGAGCTGGAGCACGTCACACGC CGCACCTTGAGCATAGACAAGGGTTTCTGAGCTTACTGTCACTACTGTTCCTGCGTTCCCGGACTCTCAC CCTCCCAGTGCTAGCCACAGATCCCAGCACCGAGGGGAACTCAAGGGGAACTCGGAGCCCGGGGCTGGCT GAGGGATGTGGGGGTCTACTGTGCTGCTCTGGGGCTCTTCCCTTAGCCCTCCCCCCACCCCCAGGACAGA GAGCCTGTGGGGCCCCTACCTTCCAGTCAGACCCACATTGCCTCTCCCTCACTGGCCAGCTGGCTGCCTC ACCACACGGCTGAAAAGAGCCTCACTTTTTTAGAAATAAAGTCACCTGTCTAGAGCAAAAAAAAAAAAAA AA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_008826 |
| Insert Size | 2343 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC020097, AAH20097 |
| RefSeq Size | 2732 bp |
| RefSeq ORF | 2343 bp |
| Locus ID | 18641 |
| UniProt ID | P12382 |
| Gene Summary | Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis (By similarity). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway (PubMed:26194095).[UniProtKB/Swiss-Prot Function] |
Citations (1)
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210614 | Pfkl (tGFP-tagged) - Mouse phosphofructokinase, liver, B-type (Pfkl) |
CNY 7,510.00 |
|
| MR210614 | Pfkl (Myc-DDK-tagged) - Mouse phosphofructokinase, liver, B-type (Pfkl) |
CNY 5,720.00 |
|
| MR210614L3 | Lenti ORF clone of Pfkl (Myc-DDK-tagged) - Mouse phosphofructokinase, liver, B-type (Pfkl) |
CNY 8,740.00 |
|
| MR210614L4 | Lenti ORF clone of Pfkl (mGFP-tagged) - Mouse phosphofructokinase, liver, B-type (Pfkl) |
CNY 8,740.00 |
