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Eed (NM_021876) Mouse Untagged Clone
CAT＃: MC203771
Eed (untagged) - Mouse embryonic ectoderm development (Eed), (10ug)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | ENSMUSG00000039373; l(7)5Rn; l7Rn5; lusk |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC012966
CCACGCGTCCGCCCACGCGTCCGGCCAATTGCGGATGAAACGTCTTGGCCAGGAAGGGGGTGAGGGAGCA GCCCTTTGAGTTCCGCCTCGTCTCTAGGCGCTGGTGGGGAAGGGGGACGTAGGAAACCCCTGCCCTCTGA GCCAGCAGATCCGACGCCTCGGTGCAAAGTGCCGAGAGGGCGGCGGGAAAACCGCCCGCCCGGACCAAGG GACGGGAAGCAGCCGGGAAGCCGTGCGGGAGGGCGCGCGCGCGCGCGCCCCTTTTTCAGCAGTGTGGCGG GGTCGCACGCACGCCCGCCTCGGCGGCTGGGCGCGATTTGCGACAGTGGGGGGAGCGGCGGCGGTGGCGG CGCCGGCAGCTTTGCGGCAGGCTCGGGCCGGGCGTGCTTGACGGCGGTGTGGCGGAGGCCCCGCCCGAGG CGGCAAGAACCTGGAGGGAGGCGGAGGAACATGTCCGAGAGGGAAGTGTCGACTGCGCCGGCGGGAACAG ACATGCCCGCGGCCAAGAAGCAGAAGTTGAGCAGCGACGAGAACAGCAACCCGGACCTCTCGGGAGACGA AAATGACGATGCTGTCAGTATTGAGAGTGGCACAAACACAGAACGCCCGGACACACCCACAAATACGCCA AATGCACCAGGAAGGAAAAGCTGGGGAAAGGGAAAATGGAAGTCAAAGAAATGCAAATATTCTTTCAAAT GTGTGAACAGCCTCAAGGAAGATCATAACCAGCCATTGTTTGGAGTTCAGTTTAACTGGCACAGTAAAGA AGGAGACCCTCTGGTGTTTGCAACTGTGGGAAGCAACAGAGTAACCTTATACGAATGCCATTCACAGGGG GAGATACGGTTATTGCAGTCCTATGTCGATGCTGATGCAGATGAAAACTTTTACACTTGTGCATGGACCT ATGATAGCAACACCAGCCACCCTCTATTAGCAGTTGCTGGATCTAGAGGCATTATAAGAATAATTAATCC TATAACAATGCAGTGTATAAAGCACTATGTTGGCCATGGAAATGCTATCAATGAGCTGAAATTCCACCCA CGAGACCCAAACCTTCTCCTGTCAGTAAGTAAAGATCATGCTTTACGGTTATGGAATATCCAAACAGACA CTCTTGTGGCAATATTCGGAGGTGTGGAAGGGCACAGAGATGAAGTTCTGAGTGCTGATTATGATCTTTT GGGTGAAAAAATAATGTCCTGTGGTATGGATCACTCTCTTAAACTGTGGAGAATCAACTCAAAGAGGATG ATGAATGCAATTAAGGAGTCTTATGATTATAACCCAAACAAAACTAACAGGCCATTTATTTCCCAGAAAA TCCACTTTCCTGACTTTTCTACCAGAGACATACATAGGAATTATGTTGATTGTGTGCGATGGTTAGGCGA TTTGATACTTTCCAAGTCTTGTGAAAATGCCATTGTATGCTGGAAACCTGGCAAAATGGAGGATGATATA GATAAAATTAAACCTAGTGAGTCTAATGTGACTATTCTTGGGCGATTTGATTACAGCCAGTGTGACATTT GGTACATGAGGTTTTCTATGGATTTCTGGCAAAAGATGCTTGCATTGGGCAATCAGGTTGGCAAACTGTA TGTTTGGGATTTAGAAGTAGAAGATCCTCATAAAGCCAAATGCACAACACTGACCCATCATAAATGTGGC GCGGCTATTCGACAAACCAGTTTCAGTAGGGATAGCAGCATCCTCATAGCTGTCTGCGATGATGCCAGCA TTTGGCGATGGGATCGACTTCGATAAACTATTTTTTCCTAGTAAAAATTAGTCTGTTGTCTGTAAAATAG AAGTAATGTATCTTGCTAGTAAGGGCACATAGAGCATTTAGACTTGTTTTTCAGCATTCGATCAGGCTGA GCTGAATGTAGTGATGTTCACATTGTTTACATTCTTTGTACTGTCTTCCTGCTCGGACTTTACTGCTTTT AATAAAAATTTATTTTTGTAAAGCTGTGTTATTTTTATTGTGATGGAAACAAACTGGAAGAAATAAAGTT GTACCATAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_021876 |
| Insert Size | 1326 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC012966, AAH12966 |
| RefSeq Size | 2054 bp |
| RefSeq ORF | 1326 bp |
| Locus ID | 13626 |
| UniProt ID | Q921E6 |
| Gene Summary | Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark (By similarity). The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (By similarity). Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8. Plays a role in X chromosome inactivation (XCI), in which one of the two X chromosomes in female mammals is transcriptionally silenced to equalize X-linked gene dosage with XY males. Required for stable maintenance of XCI in both embryonic and extraembryonic tissues. May prevent transcriptional activation of facultative heterochromatin during differentiation. Required for development of secondary trophoblast giant cells during placental development. May regulate hippocampal synaptic plasticity in the developing brain.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG207038 | Eed (tGFP-tagged) - Mouse embryonic ectoderm development (Eed) |
CNY 7,088.00 |
|
| MR207038 | Eed (Myc-DDK-tagged) - Mouse embryonic ectoderm development (Eed) |
CNY 5,488.00 |
|
| MR207038L3 | Lenti ORF clone of Eed (Myc-DDK-tagged) - Mouse embryonic ectoderm development (Eed) |
CNY 5,800.00 |
|
| MR207038L4 | Lenti ORF clone of Eed (mGFP-tagged) - Mouse embryonic ectoderm development (Eed) |
CNY 5,800.00 |
