Gaa (NM_008064) Mouse Untagged Clone

CAT#: MC203732

Gaa (untagged) - Mouse glucosidase, alpha, acid (Gaa), transcript variant 1, (10ug)



  "NM_008064" in other vectors (5)

CNY 10,480.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms E430018M07Rik
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC010210
GCTGCCCGCTGAGCCTGGGGGTCTTCGGCCTGGAGGGTGATTGCGCAGGTGAGCTGGGCGAGCTGGTGAA GGCTGTCTCCGGCCCCAGAGCCTCCGGAGGAGCGGCCTCCTCCAGGCAGGCAGGGCCGACTGTCAGCGAG TTCCTGCTTTGGAGAATCGAGCAGAGACACGCGGCATAGGCCTTCAGAAGTATTCATGCTGCCCCGAACC AACAGGCTTTCACCATGAATATACGGAAGCCCCTCTGTTCGAACTCCGTGGTTGGGGCCTGCACCCTTAT CTCTCTGACTACAGCGGTCATCCTGGGTCATCTCATGCTTCGGGAGTTAATGCTGCTTCCCCAAGACCTT CATGAGTCCTCTTCAGGACTGTGGAAGACGTACCGACCTCACCACCAGGAAGGTTACAAGCCAGGGCCTC TGCACATCCAGGAGCAGACTGAACAGCCCAAAGAAGCACCCACACAGTGTGATGTGCCCCCCAGCAGCCG CTTTGACTGTGCCCCCGACAAAGGCATCTCACAGGAGCAATGCGAGGCCCGCGGCTGCTGCTATGTCCCA GCAGGGCAGGTGCTGAAGGAGCCGCAGATAGGGCAGCCCTGGTGTTTCTTCCCTCCCAGCTACCCAAGCT ACCGTCTAGAGAACCTGAGCTCTACAGAGTCGGGGTACACAGCCACCCTGACCCGTACCAGCCCGACCTT CTTCCCAAAGGATGTGCTGACCTTACAGCTGGAGGTGCTGATGGAGACAGACAGCCGCCTCCACTTCAAG ATCAAAGATCCTGCTAGTAAGCGCTACGAAGTGCCCCTGGAGACCCCACGTGTGCTGAGCCAGGCACCAT CCCCACTTTACAGCGTGGAATTCTCAGAGGAACCCTTTGGAGTGATCGTTCGTAGGAAGCTTGGTGGCCG AGTGTTGCTGAACACAACCGTGGCCCCCCTGTTCTTCGCTGACCAGTTCCTGCAGCTGTCCACTTCCCTG CCCTCCCAGCACATCACAGGCCTGGGGGAACACCTCAGCCCACTCATGCTCAGCACCGACTGGGCTCGTA TCACCCTCTGGAACCGGGACACACCACCCTCGCAAGGTACCAACCTCTACGGGTCACATCCTTTCTACCT GGCACTGGAGGACGGTGGCTTGGCTCACGGTGTCTTCTTGCTAAACAGCAATGCCATGGATGTCATCCTG CAACCCAGCCCAGCCCTAACCTGGAGGTCAACGGGCGGGATCCTGGATGTGTATGTGTTCCTAGGCCCAG AGCCCAAGAGCGTTGTGCAACAATACCTGGATGTTGTGGGATACCCCTTCATGCCTCCATACTGGGGCCT CGGCTTCCACCTCTGCCGCTGGGGCTACTCCTCGACCGCCATTGTCCGCCAGGTAGTGGAGAACATGACC AGGACACACTTCCCGCTGGATGTGCAATGGAATGACCTGGACTACATGGACGCCCGAAGAGACTTCACCT TCAACCAGGACAGCTTTGCCGACTTCCCAGACATGGTGCGGGAGCTGCACCAGGGTGGCCGGCGCTACAT GATGATCGTGGACCCTGCCATCAGCAGCGCAGGCCCTGCTGGGAGTTACAGGCCCTACGACGAGGGTCTG CGGAGGGGTGTGTTCATCACCAACGAGACTGGGCAGCCGCTGATTGGGAAGGTTTGGCCCGGAACCACCG CCTTCCCTGATTTCACCAACCCTGAGACCCTTGACTGGTGGCAGGACATGGTGTCTGAGTTCCACGCCCA GGTGCCCTTCGATGGCATGTGGCTCGACATGAACGAACCGTCCAACTTCGTTAGAGGCTCTCAGCAGGGC TGCCCCAACAATGAACTGGAGAACCCCCCCTATGTGCCCGGGGTGGTTGGCGGGATCTTGCAGGCAGCCA CCATCTGTGCCTCCAGCCACCAATTCCTCTCCACACACTACAACCTCCACAACCTGTACGGCCTCACTGA AGCTATCGCCTCCAGCAGGGCCCTGGTCAAGACTCGGGGAACACGACCCTTTGTGATCTCCCGCTCAACC TTCTCGGGCCACGGCCGGTACGCTGGTCACTGGACAGGGGATGTGCGGAGCTCTTGGGAGCATCTTGCAT ACTCTGTGCCAGACATCCTGCAGTTCAACCTGCTGGGCGTGCCCCTGGTCGGGGCGGACATCTGCGGCTT CATAGGAGACACGTCAGAAGAGCTGTGTGTGCGCTGGACCCAGTTGGGGGCCTTCTACCCCTTCATGCGG AACCACAATGACCTGAATAGCGTGCCTCAGGAGCCGTACAGGTTCAGCGAGACGGCGCAGCAGGCCATGA GGAAGGCCTTCGCCTTACGCTATGCCCTTCTGCCCTACCTGTACACTCTCTTCCACCGCGCCCACGTCAG AGGAGACACGGTGGCCCGGCCCCTCTTCCTGGAGTTCCCTGAGGATCCCAGCACCTGGTCTGTGGACCGC CAGCTCTTGTGGGGGCCGGCCCTGCTCATCACACCTGTGCTTGAGCCTGGGAAAACTGAAGTGACGGGCT ACTTCCCCAAGGGCACGTGGTACAACATGCAGGTGGTGTCAGTGGATTCCCTCGGTACTCTCCCTTCTCC ATCATCGGCTTCATCCTTCAGATCTGCTGTCCAGAGCAAGGGGCAGTGGCTGACACTGGAAGCCCCACTG GATACCATCAACGTGCACCTGAGGGAGGGGTACATCATACCGCTGCAGGGTCCCAGCCTCACAACCACGG AGTCCCGAAAGCAGCCCATGGCTCTGGCTGTGGCATTAACAGCAAGCGGCGAGGCCGATGGGGAGCTGTT CTGGGACGACGGGGAGAGCCTTGCAGTTCTGGAGCGTGGGGCCTACACACTGGTCACCTTCTCAGCCAAG AACAATACCATTGTGAACAAGTTAGTGCGTGTGACCAAGGAGGGAGCTGAGCTACAACTGAGGGAGGTGA CCGTCTTGGGAGTGGCCACAGCTCCTACCCAGGTCCTTTCCAACGGCATCCCTGTCTCCAATTTCACCTA CAGCCCTGACAACAAGAGCCTGGCCATCCCTGTCTCACTGCTGATGGGAGAGCTGTTTCAAATCAGCTGG TCCTAGGAGAGTCCGTCGTTTACAGAGGCCTCCAGGGAGGCAGAGGGAGCTTGAGCTGGCTCTGGCTGGT GGCTCCTGTAAGGACCTGCGTCCTGCTCTCCTGACACATCTTTGAGCTTTTCCCACCGTGTTGCTGCATG CGCCCCTGAAGCTCTGTGTTCTTAGGAGAGTGAGGCTCGCCTCACCTGCCCCACCCCAGCTGTCTGTCCC TCACCTGGCACTAGAGAATGTGGAGCTCGGCGTGGGGACATCGTGTCTGCACCAACATCAGGCTGTGCAG CCACTGCAGCCGCAACCCTGCAGAGACAGAGCTGGTGCCTTCACCAGGTTCCCAAGACTTGAGAAACTTA CTGTGAAGTGTACTTACTTTTAATAAAAAGGATATTGTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAA
Restriction Sites RsrII-NotI     
ACCN NM_008064
Insert Size 2862 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC010210, AAH10210
RefSeq Size 3504 bp
RefSeq ORF 2862 bp
Locus ID 14387
UniProt ID P70699
Gene Summary This gene encodes a lysosomal acid glucosidase that is involved in the degradation of glycogen. The encoded preproprotein undergoes proteolytic processing to generate a mature enzyme that cleaves alpha-1-4 and alpha-1-6 glycosidic bonds of glycogen, maltose and intermediate oligosaccharides within the lysosome. Mice lacking the encoded protein exhibit symptoms similar to human Pompe syndrome such as accumulation of glycogen in cardiac and skeletal muscle lysosomes resulting in reduced mobility and strength. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2015]
Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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