Chrna4 (NM_015730) Mouse Untagged Clone

CAT#: MC203635

Chrna4 (untagged) - Mouse cholinergic receptor, nicotinic, alpha polypeptide 4 (Chrna4), (10ug)



  "NM_015730" in other vectors (4)

CNY 4,688.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Acra-4; Acra4; EBN1; ENFL1
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC053013
CGAGCGGCCGCGACACGGGGCATGAAGTTGGGTGCGCGCGGGTCTCGGAGCGGAGGCGCGGTACTGCCGG GAGCCGCCCTCGTCTAGAGCCCGTTCTGTGAGCCATGGAGATCGGGGGCTCCGGGGCGCCGCCGCCGCTG CTGCTCCTGCCGCTCCTGCTGCTCTTAGGGACCGGCCTCTTGCCTGCTAGCAGCCACATAGAGACCCGGG CCCATGCGGAGGAGCGGCTCCTGAAGAGACTCTTCTCTGGCTACAACAAGTGGTCTCGGCCAGTAGCCAA TATCTCAGATGTGGTCCTTGTCCGCTTTGGCTTGTCGATTGCTCAGCTCATTGATGTGGATGAGAAAAAC CAGATGATGACGACCAACGTGTGGGTGAAGCAGGAGTGGCATGACTACAAACTGCGCTGGGACCCTGGTG ACTACGAGAATGTCACCTCCATCCGCATCCCATCTGAACTCATCTGGAGGCCTGACATCGTCCTCTACAA CAACGCGGACGGGGACTTTGCAGTCACCCACCTAACCAAAGCCCACCTGTTCTATGATGGGCGTGTGCAG TGGACACCCCCGGCCATCTATAAGAGCTCCTGCAGCATCGACGTCACCTTCTTCCCCTTCGACCAGCAGA ACTGTACCATGAAGTTTGGGTCCTGGACCTACGACAAGGCCAAGATTGACTTGGTGAGCATGCACAGCCG TGTGGACCAACTGGACTTCTGGGAAAGTGGGGAGTGGGTCATTGTGGATGCCGTGGGCACCTACAACACC AGGAAGTATGAATGCTGTGCCGAGATCTATCCTGACATCACCTACGCCTTCATCATCCGCCGACTGCCAC TGTTCTACACCATCAACCTTATCATCCCGTGCCTGCTCATCTCCTGCCTCACCGTGCTGGTCTTCTATCT GCCCTCGGAGTGCGGCGAGAAGGTCACGCTGTGCATCTCGGTGCTGCTTTCTCTCACCGTCTTCCTGCTG CTCATCACCGAGATCATCCCGTCCACCTCGCTGGTCATCCCGCTCATCGGCGAGTACCTGCTCTTCACCA TGATCTTCGTCACCCTCTCCATTGTCATCACGGTCTTCGTGCTCAATGTACACCACCGCTCACCACGCAC ACACACCATGCCCGCCTGGGTGCGCAGAGTCTTCCTGGACATTGTGCCCCGTCTCCTCTTCATGAAGCGC CCATCTGTGGTCAAAGACAACTGCCGGAGACTTATCGAATCCATGCACAAGATGGCCAACGCCCCTCGTT TCTGGCCAGAGCCTGAGAGTGAGCCCGGCATCTTGGGTGACATCTGCAACCAAGGCCTGTCACCTGCCCC AACTTTCTGCAACCGCATGGACACAGCAGTCGAGACCCAGCCTACATGCAGGTCACCCTCCCACAAGGTC CCTGACTTGAAGACATCAGAGGTTGAGAAGGCCAGTCCCTGTCCATCACCTGGCTCTTGTCACCCACCCA ATAGCAGTGGGGCCCCAGTGCTCATCAAAGCCAGGTCCCTGAGCGTCCAGCATGTGCCCAGCTCCCAGGA AGCAGCCGAGGGCAGCATCCGCTGCCGGTCTCGGAGTATCCAGTACTGTGTTTCCCAAGATGGAGCTGCT TCCCTGACTGAGAGCAAGCCCACTGGCTCCCCAGCCTCCCTGAAGACCCGTCCATCCCAGCTTCCAGTGT CAGACCAGACCTCTCCATGCAAATGCACATGCAAGGAACCATCTCCTGTGTCCCCCATCACTGTGCTCAA GGCTGGAGGCACCAAAGCACCTCCCCAACACCTGCCCCTGTCACCAGCCCTGACACGGGCAGTAGAAGGC GTCCAGTACATTGCAGACCACCTCAAGGCAGAAGACACAGACTTCTCGGTGAAGGAGGACTGGAAATACG TGGCCATGGTCATTGACCGAATCTTCCTCTGGATGTTCATCATTGTCTGCCTTCTGGGCACTGTGGGACT CTTCCTGCCTCCATGGTTGGCTGGTATGATCTAGGGAATAGCGGCACCTAGCTCCCAGGTCTCTACAGGG CCATGCGACTCGTCAGTCACCCACATCTTCCAAACCGGCCATGAGACACCTAGGAGAGAGAGATGCTGCC CTGGTTGACCCTGGTTCTAGTCAGGCCACAGGCCTGGTTGGAGCTAGTTGAGGACTGATATAGTTACAGG CTGAGTCCCTCATTAAAGTTTCTCCAGAGCAAGTGACAGTCACTCCCTGGCTTACAGACAGCACACACCC ATCTGTGTCACAGAGAATGATCCAGTGTTGATCTCAGTTGTCCTTTGAGGCCAAAACAATTCATCCCCCT TCAGGAACCAGAGCCCCTCGTGCTGTGGGATTCCTACGGCCCAGGAAATTCCCATGGTGCTCTGCTGGCC ACACCCTCTCCCTCCCCATAATGTGGTTCCCTCAACCCTCCAGGCTGGGCTGCTCTCTGACTCAAAGGTG TCAGATGTAAGCCCCGGCAGGTTTTTATTTTGTTTAGGTTGAAGCGAATTGGTAAGAAATAGAGCAGTGA GATATGTGGATGAGTCCCACTCACAGGTGAATGAGTGCAGGGTCTCACAGGAAGAGTGAGGCACCACAGG ACTCCTGCTTCCATCTCAGGGTCACAGGCATCAATCATGAGCATTTCCTAGGGTCCATAAACCCGAGGAG GGCAAGGGCATAGAGGGTCTCAGGGTTGTGATGGAGCCAAATCCTGTCCAGGGCCTGGGCCGTTCATCCC CTCATGGATCTTCCTTGATATCCCTGTATGTTTCTGCCTCTCTGGAATTAGAAGACTGAAAGTAAGATTT CTCATCACGGTCCTGTGGTGTGGCCACAGTTCACCTGAGCACATCTCTCTAGACCAGTAGGAGTGGTGCG AAGCCCCTTCAATGTTGTAGAATAGCGTGAGCTGCCAAGAGACTTCTAAGCAAAACAGGCTCTGTGACTC ATTTTTCGAGGGCCATCGACCAAGTCTTAGGGGTGCCTCACCCTGTCTGCCTTGCACTTAGGGAAGACCC GAGAGGTTCCTCTTCCCCTTCCCAAGATGGCACCAGGCAACCTAGAGAACCCACCGTGGTGGGATGGGAG AACGAACATGCTGTGCACATCTCTATGAGATTCCACTGAGTGAAGCCCAGAACATGGGAGGCATGCAAGA AATCACCCTGTGTCGTGGTCCCAGTTGACCCTCCGCTGTCTCCACCAGCCAGGTGGGTTTCACAGAGCTG GGCCCTGCACCCTCAGCCAAGCTGTTCTAGGCCCTGAAGCTGAGGTCCCTGTTTGGATAGTCCTGGGGAC TGCAGAATGAAAGAAGAACTTAATGAACGCACCAAGCCTCCAGTAGGTACGGCTGCCACCTCCGTGGTAT GACTTGCCCATCCCAGCTGAATGAGGATGTCAGGAAGGAGGTATGCCAGAGGGCCAGCATTGCCTTTACC TGACTACCTACAGGCAAATCCACCTTTAAACACAGAGCTGCTGGACATCCAGGGTGCTGGTGGGAAAGGA ACTCCACACTGGGAGCCCCAGGCCATTCCTATGAACAGGAAGGGGATGCAGAGGCCTGGTCTCTGAACTC TGGATATTGTTCCAGGTCTTCCCTAGAGTCCTAAGGGCATCGAGGATCCCATCTGCCATGTTTCAGTCTG CCCTCCACTGACTGACTAGATCTCTAGCCCCTATATTGGAACTGTCGGGATGCTGCAAGATGACCCTGGT GGGGAAATTCATGCCAGAATCTGGGACCAAGGGGAACACAAGCCCCAGTGATGAAGACAGCAGGTAACAC CTGACAGATGTGTGTTCTACCATTATGGTGCATACGTGGCTCCAACCACAAGAAATGCAGACAACAGTGG AGATCAGGGCAAGGCCATTGTGACATGGAACAGGACCGCTGTGCTGGTCTCTTCAGGGTTAGGAAAACTG AACTGCTGGGATGCTCCTGACAGGCTACCCACTTCCCCCTACCCCCCAACACACATTCACAAGCCAGAAA AGGAAAATAAAACACCGTGTTCTCCCCATTCCCACTCAGCCGGCCTTTTGTCTGCCTGCTTCCAGTGTTG ATATGTGTTCAAGATAAAGTTCAGTTAGGGCAGAATGCTTGATTTAAGACTTTTGAACCAGTGAGCTTTA AAGAACAGAGACTGTGTGGCCCCAGCCCCTCTGATACGTAGACATTATCTCCCCAAAGCTCCCAGTCCTC CCAGTCTACCCCATCCCATTAGACAGCATCAACTCAAATGTGAGTCTTGGAGACCAGTTCGGCTAGCCAT CATATGTCTGGGAATCCCATATTGGACTCTGCAATGTCTGGCTTCTTTCGCTTGTGTGGCCAAGGCTCAT CTGCGGTGTTGTGTGTGATAGACTCATTGCTGTTGTGTGCTTGTTGGATCTTAGTTGTTTCTGTCTGAAT AAACCGAGTCGTGGTGTCTTCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites AscI-NotI     
ACCN NM_015730
Insert Size 1890 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC053013, AAH53013
RefSeq Size 4508 bp
RefSeq ORF 1890 bp
Locus ID 11438
UniProt ID O70174
Gene Summary After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodium ions.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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