Mgme1 (NM_028984) Mouse Untagged Clone
CAT#: MC203615
Mgme1 (untagged) - Mouse RIKEN cDNA 8430406I07 gene (8430406I07Rik), (10ug)
CNY 3,656.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 8430406I07Rik; AI426476 |
Vector | PCMV6-Kan/Neo |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>BC063088
GGGATGGCGCCTCACAGGTGCGACGGGAACGCCGTAGTGCATGCTGGGGCCGCCTCGCTTTGGGAAAGCG CGGGACCGATGAAAATACGAACAGAACACACTTTTAAACTTTCCAGACTAGAGAAAAGTTAGAGGGGCGA AGTCTAAAAATGAAACTGCCTCTGACCTTCTGCAGGCTGCTTAGCAGGTTAAACAGATTTTCTGTAAAAG CAAGCCCTCCTGTGAGTTTCTCCACTTTCTCTTATTTGTGTAGCCAGAAGAAGAAAAACTCTTACGAAGC AGTAGACCAAGCGAAGTACTCTCGTTTAGTACGCTCTGTCTTGTCCAGAGGCCCGGCCCAGACTCCAGAG TCGTTGTTCAAGGAAGATGATGTACTGTATGGACCAGTGAGTAAGCATAAGGCTGCAGAGCCGGAGCCAC AGGCCAGAGTCCCACAACACTGCTTTCCTATCTTCAATGAAGAGAGAACCGGGAAACCACACACAGATGC TTCTTCAAGCCCTTTGAAGATCCCTTTGCAAAGGAACTCGATACCCAGTGTGACCCGCATCCTTCAGCAG ACCATGCCACCTGAACAGAGCTTCTTTTTGGAGAGGTGGAAAGAGCGGATGGTTCTGGAGCTGGGAGAAG ACGGGTTTGCAGAATATACTTCAAATGTGTTTTTACAAGGCAAACAGTTCCATAAAGCCTTGGAAAGCAT ACTGTCACCCCAGGAGAACCTAACAGGGGGAGAAGAGCACCCCCAGTGTGGCTACATCGAAAGCATCCAG CATATTCTGACAGAAATCAGTGGTGTGCAAGCTCTGGAGAGTGCCGTCCAGCATGAGGCCTTGAAGTATG TAGGGCTGCTGGACTGTGTGGCTGAGTACCGGGGCAAGCTGTGTGTGATTGATTGGAAGACATCAGAAAA ACCAAAACCTTTAATTCGAAATACATATGACAACCCGCTGCAAGTTGTGGCGTACATGGGTGCCGTAAAC CATGATGCCCACTACAGTTTTCAGGTTCAGTGTGGATTAATTGTGGTGGCCTATAAGGATGGGTCCCCTG CGCACCCTCACTTCATGGATGAAGAGCTCTGTTCCAAGTATTGGGCCAAGTGGCTTCTCCGACTAGAAGA ATATACAGAAAAGCAAAAGAACCTGAGCGCTCCAGAGCCAGCGTAGAGGCCCGGGATTGCTTGGCGATGC TGCGCGGCGATCTCTGTTTGGGAGATGCACTGCTGTCCACTGAGTTTCTAAAGAGGAATGCGGGGAACTG AGGGCTACATTAACACAAGCTGAAGGTGTGGTGGGTTGGGATGTACCCACCGAGAAGCCAGAGAGTCTGG AGGTCAGCGCCTGACTCACCAGCTGCACGACCTCTGGCTGTTTCTCACCTGAGAGGAGGACACTGGGATT GGCAGCTGCCCAGGGACTTCTGGGCTCCCTTGCAAACCTCTGTGTCCCTGGCAGGATGCCCAGTCTTTGA ATTGAGATCAGAAGGCCAAATGAAGCATCATGTGCCACACATGTCCCTAGAGTTCTCAGGGATGGGAGGG GCCCTGCACCCTCAGGGTATACAGAGAGAGAGAGAGTGAGCAAGCAAGCACGTAGGCATCGCCACCACAG TTTCCTAGAAGGTGTTTTATACACTCTGTGTCATTAGGAAAAACTTATTTCAGCCTAAGGAAGCCTGGCT GCCTAGAGAGCTGGGGCAGGCAATACATCATTGGCTAGGTCCTTTTCCTTACCAGAGAAGCCCCAGCAGC AGGAAGCCTGAGTGTGGAAGTCACCGTCGCTAGGGTTTGGGTATCTATTTCTGGTGTCAGCAGGCCATGG ACTACAAAAAATACTGTTGTGGAACTTGTTAACAAAGAGGAACAATGGTAAAACTTTTTTTCTGTGTTTG ATAAGTAACAACAAAAAAAAAATCATTGGCTGTTCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAA |
Restriction Sites | RsrII-NotI |
ACCN | NM_028984 |
Insert Size | 1017 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC063088, AAH63088 |
RefSeq Size | 1982 bp |
RefSeq ORF | 1017 bp |
Locus ID | 74528 |
UniProt ID | Q9CXC3 |
Gene Summary | Metal-dependent single-stranded DNA (ssDNA) exonuclease involved in mitochondrial genome maintenance. Has preference for 5'-3' exonuclease activity but is also capable of endoduclease activity on linear substrates. Necessary for maintenance of proper 7S DNA levels. Probably involved in mitochondrial DNA (mtDNA) repair, possibly via the processing of displaced DNA containing Okazaki fragments during RNA-primed DNA synthesis on the lagging strand or via processing of DNA flaps during long-patch base excision repair (By similarity). Specifically binds 5-hydroxymethylcytosine (5hmC)-containing DNA in stem cells.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All four variants encode the same protein. Sequence Note:. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG205034 | Mgme1 (tGFP-tagged) - Mouse RIKEN cDNA 8430406I07 gene (8430406I07Rik) |
CNY 3,140.00 |
|
MR205034 | Mgme1 (Myc-DDK-tagged) - Mouse RIKEN cDNA 8430406I07 gene (8430406I07Rik) |
CNY 3,656.00 |
|
MR205034L3 | Lenti ORF clone of Mgme1 (Myc-DDK-tagged) - Mouse RIKEN cDNA 8430406I07 gene (8430406I07Rik) |
CNY 4,750.00 |
|
MR205034L4 | Lenti ORF clone of Mgme1 (mGFP-tagged) - Mouse RIKEN cDNA 8430406I07 gene (8430406I07Rik) |
CNY 4,750.00 |