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Egf (NM_010113) Mouse Untagged Clone
CAT＃: MC203560
Egf (untagged) - Mouse epidermal growth factor (Egf), (10ug)
CNY 12,720.00
货期*
现货
规格
Product images
经常一起买 (2)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI790464 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC060741
AGGGATTCCTATCTGTATATAGGGAAGGAATCCTATCTGCATATTTCGTTGTTAGCACCATCCCTCATCC CGGTGGGGCTTGGAACTTTCCATCAATTCTTTCCTGTCTCGTTTCTCTTTCATCCTTTGCCTGGTTGTGC CTGTCTCAGGGAGAAATCAGTCACCTGCAGGCCTTGCAGGGCTCTTAGGCTCTGGGAAATTTGTCATACG GGTGTCAGGTACTTCTTATTGCTGTCCAAAGGGAAAAAAAAAGTGAGACAAAGAACTCTCCCGGAGCCTT TCCGGCTGCACTCAGAGGCTCTCGAGAGGTGCAGAAGGACCTGGAAAGGCAGCTAAATAAAAGATGCCCT GGGGCCGAAGGCCAACCTGGCTGTTGCTCGCCTTCCTGCTGGTGTTTTTAAAGATTAGCATACTCAGCGT CACAGCATGGCAGACCGGGAACTGTCAGCCAGGTCCTCTCGAGAGAAGCGAGAGAAGCGGGACTTGTGCC GGTCCTGCCCCCTTCCTAGTTTTCTCACAAGGAAAGAGCATCTCTCGGATTGACCCAGATGGAACAAATC ACCAGCAATTGGTGGTGGATGCTGGCATCTCAGCAGACATGGATATTCATTATAAAAAAGAGAGACTCTA TTGGGTGGATGTAGAAAGACAAGTTTTGCTAAGAGTTTTCCTTAACGGGACAGGACTAGAGAAAGTGTGC AATGTAGAGAGGAAGGTGTCTGGGCTGGCCATAGACTGGATAGATGATGAAGTTCTCTGGGTAGACCAAC AGAACGGAGTCATCACCGTAACAGATATGACAGGGAAAAATTCCCGAGTTCTTCTAAGTTCCTTAAAACA TCCGTCAAATATAGCAGTGGATCCAATAGAGAGGTTGATGTTTTGGTCTTCAGAGGTGACCGGCAGCCTT CACAGAGCACACCTCAAAGGTGTTGATGTAAAAACACTGCTGGAGACAGGGGGAATATCGGTGCTGACTC TGGATGTCCTGGACAAACGGCTCTTCTGGGTTCAGGACAGTGGCGAAGGAAGCCACGCTTACATTCATTC CTGTGATTATGAGGGTGGCTCCGTCCGTCTTATCAGGCATCAAGCACGGCACAGTTTGTCTTCAATGGCC TTTTTTGGTGATCGGATCTTCTACTCAGTGTTGAAAAGCAAGGCGATTTGGATAGCCAACAAACACACGG GGAAGGACACGGTCAGGATTAACCTCCATCCATCCTTTGTGACACCTGGAAAACTGATGGTAGTACACCC TCGTGCACAGCCCAGGACAGAGGACGCTGCTAAGGATCCTGACCCCGAACTTCTCAAACAGAGGGGAAGA CCATGCCGCTTCGGTCTCTGTGAGCGAGACCCCAAGTCCCACTCGAGCGCATGCGCTGAGGGCTACACGT TAAGCCGAGACCGGAAGTACTGCGAAGATGTCAATGAATGTGCCACTCAGAATCACGGCTGTACTCTTGG GTGTGAAAACACCCCTGGATCCTATCACTGCACATGCCCCACAGGATTTGTTCTGCTTCCTGATGGGAAA CAATGTCACGAACTTGTTTCCTGCCCAGGCAACGTATCAAAGTGCAGTCATGGCTGTGTCCTGACATCAG ATGGTCCCCGGTGCATCTGTCCTGCAGGTTCAGTGCTTGGGAGAGATGGGAAGACTTGCACTGGTTGTTC ATCGCCTGACAATGGTGGATGCAGCCAGATCTGTCTTCCTCTCAGGCCAGGATCCTGGGAATGTGATTGC TTTCCTGGGTATGACCTACAGTCAGACCGAAAGAGCTGTGCAGCTTCAGGACCACAGCCACTTTTACTGT TTGCAAATTCCCAGGACATCCGACACATGCATTTTGATGGAACAGACTACAAAGTTCTGCTCAGCCGGCA GATGGGAATGGTTTTTGCCTTGGATTATGACCCTGTGGAAAGCAAGATATATTTTGCACAGACAGCCCTG AAGTGGATAGAGAGGGCTAATATGGATGGGTCCCAGCGAGAAAGACTGATCACAGAAGGAGTAGATACGC TTGAAGGTCTTGCCCTGGACTGGATTGGCCGGAGAATCTACTGGACAGACAGTGGGAAGTCTGTTGTTGG AGGGAGCGATCTGAGCGGGAAGCATCATCGAATAATCATCCAGGAGAGAATCTCGAGGCCGCGAGGAATA GCTGTGCATCCAAGGGCCAGGAGACTGTTCTGGACGGACGTAGGGATGTCTCCACGGATTGAAAGCGCTT CCCTTCAAGGTTCCGACCGGGTGCTGATAGCCAGCTCCAATCTACTGGAACCCAGTGGAATCACGATTGA CTACTTAACAGACACTTTGTACTGGTGTGACACCAAGAGGTCTGTGATTGAAATGGCCAATCTGGATGGC TCCAAACGCCGAAGACTTATCCAGAACGACGTAGGTCACCCCTTCTCTCTAGCCGTGTTTGAGGATCACC TGTGGGTCTCGGATTGGGCTATCCCATCGGTAATAAGGGTGAACAAGAGGACTGGCCAAAACAGGGTACG TCTTCAAGGCAGCATGCTGAAGCCCTCGTCACTGGTTGTGGTCCATCCATTGGCAAAACCAGGTGCAGAT CCCTGCTTATACAGGAATGGAGGCTGTGAACACATCTGCCAAGAGAGCCTGGGCACAGCTCGGTGTTTGT GTCGTGAAGGTTTTGTGAAGGCCTGGGATGGGAAAATGTGTCTCCCTCAGGATTATCCAATCCTGTCAGG TGAAAATGCTGATCTTAGTAAAGAGGTGACATCACTGAGCAACTCCACTCAGGCTGAAGTACCAGACGAT GATGGGACAGAATCTTCCACACTAGTGGCTGAAATCATGGTGTCAGGCATGAACTATGAAGATGACTGTG GTCCCGGGGGGTGTGGAAGCCATGCTCGATGCGTTTCAGACGGAGAGACTGCTGAGTGTCAGTGTCTGAA AGGGTTTGCCAGGGATGGAAACCTGTGTTCTGATATAGATGAGTGTGTGCTGGCTAGATCGGACTGCCCC AGCACCTCGTCCAGGTGCATCAACACTGAAGGTGGCTACGTCTGCAGATGCTCAGAAGGCTACGAAGGAG ACGGGATCTCCTGTTTCGATATTGACGAGTGCCAGCGGGGGGCGCACAACTGCGCTGAGAATGCCGCCTG CACCAACACGGAGGGAGGCTACAACTGCACCTGCGCAGGCCGCCCATCCTCGCCCGGACGGAGTTGCCCT GACTCTACCGCACCCTCTCTCCTTGGGGAAGATGGCCACCATTTGGACCGAAATAGTTATCCAGGATGCC CATCCTCATATGATGGATACTGCCTCAATGGTGGCGTGTGCATGCATATTGAATCACTGGACAGCTACAC ATGCAACTGTGTTATTGGCTATTCTGGGGATCGATGTCAGACTCGAGACCTACGATGGTGGGAGCTGCGT CATGCTGGCTACGGGCAGAAGCATGACATCATGGTGGTGGCTGTCTGCATGGTGGCACTGGTCCTGCTGC TCCTCTTGGGGATGTGGGGGACTTACTACTACAGGACTCGGAAGCAGCTATCAAACCCCCCAAAGAACCC TTGTGATGAGCCAAGCGGAAGTGTGAGCAGCAGCGGGCCCGACAGCAGCAGCGGGGCAGCTGTGGCTTCT TGTCCCCAACCTTGGTTTGTGGTCCTAGAGAAACACCAAGACCCCAAGAATGGGAGTCTGCCTGCGGATG GTACGAATGGTGCAGTAGTAGATGCTGGCCTGTCTCCCTCCCTGCAGCTCGGGTCAGTGCATCTGACTTC ATGGAGACAGAAGCCCCACATAGATGGAATGGGCACAGGGCAAAGCTGCTGGATTCCACCATCAAGTGAC AGAGGACCCCAGGAAATAGAGGGAAACTCCCACCTACCCTCCTACAGACCTGTGGGGCCGGAGAAGCTGC ATTCTCTCCAGTCAGCTAATGGATCGTGTCACGAAAGGGCTCCAGACCTGCCACGGCAGACAGAGCCAGT TCAGTAGAAACTGGGAGTAGACAGAAGGTACAGAAGGGAAAATAACAAACCAGGCTGATGATGGTAGAGT GCTACAGACTTGGTACTCCAGTTTCCACGGCTAATCACTGCTCGCTCAGGGTCCTGAAGATAGCTGCACA GCTGCAGAGCTGCACAGCGGGATAGCTGCGACTTTTGCTTCTTGCTTTAAGCAGTTCCACTGAAGATACT CAAAAGAGAAGTGGAGAAAATCATTAGAAACCAAAGTCAAGACATTCATATATAAGCTGTGTCTTCTTCA CTGGACGGTTTGCCTCTTTTCCTTTTGCCTCAGAAGGAGTGGGTTAAAGCAGGTGACCCCATGCTCTGTC AACCCCTGAATAAATGATGTGATCTACATAGAAGTCTTAGCTCACTCTCAGGAACGCTTGGAACACTATA ACTTTTGCTATGATATACTGCCAAGTGTGGCCCATGCTCATAATTGTGCCTTCTGAATTGTGATAAATTA GTGAAAAAACTGTAACTTAGAATCTGATTTATTCAGGATTAGATCATCTTTTTATACTATAAAAATCTTC GAATGAAAATATTTAACTTTAAAAACATTACCTTAATCATTGTCTTTTCTTCTTGAAGTCTTTCCCAGTG AAAACGCTCAATTCTGCTGTTTCCATAGAATTTTTAATTTATTTTAAGACATGAGATTGTAAACAAATTG CTTGATTTATTTTATCCTAATTATTTAAATAAAATCACCCTAAAGCATCAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_010113 |
| Insert Size | 3654 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC060741, AAH60741 |
| RefSeq Size | 4789 bp |
| RefSeq ORF | 3654 bp |
| Locus ID | 13645 |
| UniProt ID | P01132 |
| Gene Summary | This gene encodes epidermal growth factor (EGF), the founding member of the EGF family of growth factors that are implicated in cell proliferation and differentiation. The encoded protein can localize to the membrane and function in juxtacrine signaling or undergo proteolytic processing to generate a soluble form of the hormone. Mice lacking the encoded protein do not exhibit an abnormal phenotype but transgenic mice overexpressing the encoded protein exhibit hypospermatogenesis. [provided by RefSeq, Jul 2016] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211815 | Egf (tGFP-tagged) - Mouse epidermal growth factor (Egf) |
CNY 13,400.00 |
|
| MR211815 | Egf (Myc-DDK-tagged) - Mouse epidermal growth factor (Egf) |
CNY 12,720.00 |
|
| MR211815L3 | Lenti ORF clone of Egf (Myc-DDK-tagged) - Mouse epidermal growth factor (Egf) |
CNY 14,060.00 |
|
| MR211815L4 | Lenti ORF clone of Egf (mGFP-tagged) - Mouse epidermal growth factor (Egf) |
CNY 14,060.00 |
Customer
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