Egf (NM_010113) Mouse Untagged Clone

CAT#: MC203560

Egf (untagged) - Mouse epidermal growth factor (Egf), (10ug)



  "NM_010113" in other vectors (4)

CNY 12,720.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AI790464
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC060741
AGGGATTCCTATCTGTATATAGGGAAGGAATCCTATCTGCATATTTCGTTGTTAGCACCATCCCTCATCC CGGTGGGGCTTGGAACTTTCCATCAATTCTTTCCTGTCTCGTTTCTCTTTCATCCTTTGCCTGGTTGTGC CTGTCTCAGGGAGAAATCAGTCACCTGCAGGCCTTGCAGGGCTCTTAGGCTCTGGGAAATTTGTCATACG GGTGTCAGGTACTTCTTATTGCTGTCCAAAGGGAAAAAAAAAGTGAGACAAAGAACTCTCCCGGAGCCTT TCCGGCTGCACTCAGAGGCTCTCGAGAGGTGCAGAAGGACCTGGAAAGGCAGCTAAATAAAAGATGCCCT GGGGCCGAAGGCCAACCTGGCTGTTGCTCGCCTTCCTGCTGGTGTTTTTAAAGATTAGCATACTCAGCGT CACAGCATGGCAGACCGGGAACTGTCAGCCAGGTCCTCTCGAGAGAAGCGAGAGAAGCGGGACTTGTGCC GGTCCTGCCCCCTTCCTAGTTTTCTCACAAGGAAAGAGCATCTCTCGGATTGACCCAGATGGAACAAATC ACCAGCAATTGGTGGTGGATGCTGGCATCTCAGCAGACATGGATATTCATTATAAAAAAGAGAGACTCTA TTGGGTGGATGTAGAAAGACAAGTTTTGCTAAGAGTTTTCCTTAACGGGACAGGACTAGAGAAAGTGTGC AATGTAGAGAGGAAGGTGTCTGGGCTGGCCATAGACTGGATAGATGATGAAGTTCTCTGGGTAGACCAAC AGAACGGAGTCATCACCGTAACAGATATGACAGGGAAAAATTCCCGAGTTCTTCTAAGTTCCTTAAAACA TCCGTCAAATATAGCAGTGGATCCAATAGAGAGGTTGATGTTTTGGTCTTCAGAGGTGACCGGCAGCCTT CACAGAGCACACCTCAAAGGTGTTGATGTAAAAACACTGCTGGAGACAGGGGGAATATCGGTGCTGACTC TGGATGTCCTGGACAAACGGCTCTTCTGGGTTCAGGACAGTGGCGAAGGAAGCCACGCTTACATTCATTC CTGTGATTATGAGGGTGGCTCCGTCCGTCTTATCAGGCATCAAGCACGGCACAGTTTGTCTTCAATGGCC TTTTTTGGTGATCGGATCTTCTACTCAGTGTTGAAAAGCAAGGCGATTTGGATAGCCAACAAACACACGG GGAAGGACACGGTCAGGATTAACCTCCATCCATCCTTTGTGACACCTGGAAAACTGATGGTAGTACACCC TCGTGCACAGCCCAGGACAGAGGACGCTGCTAAGGATCCTGACCCCGAACTTCTCAAACAGAGGGGAAGA CCATGCCGCTTCGGTCTCTGTGAGCGAGACCCCAAGTCCCACTCGAGCGCATGCGCTGAGGGCTACACGT TAAGCCGAGACCGGAAGTACTGCGAAGATGTCAATGAATGTGCCACTCAGAATCACGGCTGTACTCTTGG GTGTGAAAACACCCCTGGATCCTATCACTGCACATGCCCCACAGGATTTGTTCTGCTTCCTGATGGGAAA CAATGTCACGAACTTGTTTCCTGCCCAGGCAACGTATCAAAGTGCAGTCATGGCTGTGTCCTGACATCAG ATGGTCCCCGGTGCATCTGTCCTGCAGGTTCAGTGCTTGGGAGAGATGGGAAGACTTGCACTGGTTGTTC ATCGCCTGACAATGGTGGATGCAGCCAGATCTGTCTTCCTCTCAGGCCAGGATCCTGGGAATGTGATTGC TTTCCTGGGTATGACCTACAGTCAGACCGAAAGAGCTGTGCAGCTTCAGGACCACAGCCACTTTTACTGT TTGCAAATTCCCAGGACATCCGACACATGCATTTTGATGGAACAGACTACAAAGTTCTGCTCAGCCGGCA GATGGGAATGGTTTTTGCCTTGGATTATGACCCTGTGGAAAGCAAGATATATTTTGCACAGACAGCCCTG AAGTGGATAGAGAGGGCTAATATGGATGGGTCCCAGCGAGAAAGACTGATCACAGAAGGAGTAGATACGC TTGAAGGTCTTGCCCTGGACTGGATTGGCCGGAGAATCTACTGGACAGACAGTGGGAAGTCTGTTGTTGG AGGGAGCGATCTGAGCGGGAAGCATCATCGAATAATCATCCAGGAGAGAATCTCGAGGCCGCGAGGAATA GCTGTGCATCCAAGGGCCAGGAGACTGTTCTGGACGGACGTAGGGATGTCTCCACGGATTGAAAGCGCTT CCCTTCAAGGTTCCGACCGGGTGCTGATAGCCAGCTCCAATCTACTGGAACCCAGTGGAATCACGATTGA CTACTTAACAGACACTTTGTACTGGTGTGACACCAAGAGGTCTGTGATTGAAATGGCCAATCTGGATGGC TCCAAACGCCGAAGACTTATCCAGAACGACGTAGGTCACCCCTTCTCTCTAGCCGTGTTTGAGGATCACC TGTGGGTCTCGGATTGGGCTATCCCATCGGTAATAAGGGTGAACAAGAGGACTGGCCAAAACAGGGTACG TCTTCAAGGCAGCATGCTGAAGCCCTCGTCACTGGTTGTGGTCCATCCATTGGCAAAACCAGGTGCAGAT CCCTGCTTATACAGGAATGGAGGCTGTGAACACATCTGCCAAGAGAGCCTGGGCACAGCTCGGTGTTTGT GTCGTGAAGGTTTTGTGAAGGCCTGGGATGGGAAAATGTGTCTCCCTCAGGATTATCCAATCCTGTCAGG TGAAAATGCTGATCTTAGTAAAGAGGTGACATCACTGAGCAACTCCACTCAGGCTGAAGTACCAGACGAT GATGGGACAGAATCTTCCACACTAGTGGCTGAAATCATGGTGTCAGGCATGAACTATGAAGATGACTGTG GTCCCGGGGGGTGTGGAAGCCATGCTCGATGCGTTTCAGACGGAGAGACTGCTGAGTGTCAGTGTCTGAA AGGGTTTGCCAGGGATGGAAACCTGTGTTCTGATATAGATGAGTGTGTGCTGGCTAGATCGGACTGCCCC AGCACCTCGTCCAGGTGCATCAACACTGAAGGTGGCTACGTCTGCAGATGCTCAGAAGGCTACGAAGGAG ACGGGATCTCCTGTTTCGATATTGACGAGTGCCAGCGGGGGGCGCACAACTGCGCTGAGAATGCCGCCTG CACCAACACGGAGGGAGGCTACAACTGCACCTGCGCAGGCCGCCCATCCTCGCCCGGACGGAGTTGCCCT GACTCTACCGCACCCTCTCTCCTTGGGGAAGATGGCCACCATTTGGACCGAAATAGTTATCCAGGATGCC CATCCTCATATGATGGATACTGCCTCAATGGTGGCGTGTGCATGCATATTGAATCACTGGACAGCTACAC ATGCAACTGTGTTATTGGCTATTCTGGGGATCGATGTCAGACTCGAGACCTACGATGGTGGGAGCTGCGT CATGCTGGCTACGGGCAGAAGCATGACATCATGGTGGTGGCTGTCTGCATGGTGGCACTGGTCCTGCTGC TCCTCTTGGGGATGTGGGGGACTTACTACTACAGGACTCGGAAGCAGCTATCAAACCCCCCAAAGAACCC TTGTGATGAGCCAAGCGGAAGTGTGAGCAGCAGCGGGCCCGACAGCAGCAGCGGGGCAGCTGTGGCTTCT TGTCCCCAACCTTGGTTTGTGGTCCTAGAGAAACACCAAGACCCCAAGAATGGGAGTCTGCCTGCGGATG GTACGAATGGTGCAGTAGTAGATGCTGGCCTGTCTCCCTCCCTGCAGCTCGGGTCAGTGCATCTGACTTC ATGGAGACAGAAGCCCCACATAGATGGAATGGGCACAGGGCAAAGCTGCTGGATTCCACCATCAAGTGAC AGAGGACCCCAGGAAATAGAGGGAAACTCCCACCTACCCTCCTACAGACCTGTGGGGCCGGAGAAGCTGC ATTCTCTCCAGTCAGCTAATGGATCGTGTCACGAAAGGGCTCCAGACCTGCCACGGCAGACAGAGCCAGT TCAGTAGAAACTGGGAGTAGACAGAAGGTACAGAAGGGAAAATAACAAACCAGGCTGATGATGGTAGAGT GCTACAGACTTGGTACTCCAGTTTCCACGGCTAATCACTGCTCGCTCAGGGTCCTGAAGATAGCTGCACA GCTGCAGAGCTGCACAGCGGGATAGCTGCGACTTTTGCTTCTTGCTTTAAGCAGTTCCACTGAAGATACT CAAAAGAGAAGTGGAGAAAATCATTAGAAACCAAAGTCAAGACATTCATATATAAGCTGTGTCTTCTTCA CTGGACGGTTTGCCTCTTTTCCTTTTGCCTCAGAAGGAGTGGGTTAAAGCAGGTGACCCCATGCTCTGTC AACCCCTGAATAAATGATGTGATCTACATAGAAGTCTTAGCTCACTCTCAGGAACGCTTGGAACACTATA ACTTTTGCTATGATATACTGCCAAGTGTGGCCCATGCTCATAATTGTGCCTTCTGAATTGTGATAAATTA GTGAAAAAACTGTAACTTAGAATCTGATTTATTCAGGATTAGATCATCTTTTTATACTATAAAAATCTTC GAATGAAAATATTTAACTTTAAAAACATTACCTTAATCATTGTCTTTTCTTCTTGAAGTCTTTCCCAGTG AAAACGCTCAATTCTGCTGTTTCCATAGAATTTTTAATTTATTTTAAGACATGAGATTGTAAACAAATTG CTTGATTTATTTTATCCTAATTATTTAAATAAAATCACCCTAAAGCATCAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN NM_010113
Insert Size 3654 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC060741, AAH60741
RefSeq Size 4789 bp
RefSeq ORF 3654 bp
Locus ID 13645
UniProt ID P01132
Gene Summary This gene encodes epidermal growth factor (EGF), the founding member of the EGF family of growth factors that are implicated in cell proliferation and differentiation. The encoded protein can localize to the membrane and function in juxtacrine signaling or undergo proteolytic processing to generate a soluble form of the hormone. Mice lacking the encoded protein do not exhibit an abnormal phenotype but transgenic mice overexpressing the encoded protein exhibit hypospermatogenesis. [provided by RefSeq, Jul 2016]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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