United States
Germany
Japan
United Kingdom
China
Sesn1 (NM_001013370) Mouse Untagged Clone
CAT＃: MC203553
Sesn1 (untagged) - Mouse sestrin 1 (Sesn1), transcript variant 2, (10ug)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1110002G11Rik; AU044290; Pa26; Sest1 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC055753
TGAAGCGCTGCAGCGACAGATCGCGGCCGCAGCCGTCCCTTCGACATGCGCCTGGCCGCCGCGTCGAACG AGGCGTACGCAGCCTCGCTGGCTGTCTCCGAGCTGCTGAGCTGCCACCAGTGCGGCGGGGACCGCGGCCA GGACGAGGAACTTGGAATCAGAATCCCTCGGCCACTAGGACACGGACCAAGCAGGTTCATCCCAGAGAAG GAGATGCTGCAGGTGGGCAGTGAAGACGCACAGATGCATGCGTTATTTGCAGACTCCTTTGCTGCTTTGG GTCGTCTGGATAACATCACATTAGTGATGGTTTTCCATCCACAATATTTAGAAAGTTTCTTAAAGACTCA ACACTACCTACTACAAATGGATGGGCCATTACCCCTCCATTATCGGCACTACATTGGAATCATGGCGGCA GCGAGGCATCAGTGTTCCTACCTGGTGAATCTGCATGTCAGTGACTTTCTTCATGTTGGTGGGGACCCCA AGTGGCTCAATGGTTTAGAGAATGCTCCTCAAAAACTGCAGAACTTAGGAGAACTTAACAAAGTGTTAGC CCATAGGCCTTGGCTGATTACCAAAGAACATATCGAGGGCCTTCTGAAAGCAGAGGAGCACAGCTGGTCT CTCGCCGAGCTGGTCCATGCCGTGGTTCTGCTCACACACTACCACTCTCTTGCCTCCTTCACATTTGGCT GTGGAATTAGTCCAGAGATTCATTGCGATGGTGGCCACACCTTCAGACCGCCTTCTGTTAGTAACTACTG CATCTGTGACATTACAAATGGCAATCACAGTGTGGATGAGATGCAAGTCAACTCAGCAGGAAATGCTTCG GTAAGTGATTCCTTCTTTGAGGTGGAAGCTCTCATGGAAAAGATGAGGCAGTTACAGGAATGCCGAGAGG AGGAGGAGGCTAGCCAGGAGGAGATGGCGTCGCGGTTTGAGATGGAAAAGAGAGAGAGCATGTTTGTCTT CTCTTCGGACGATGATGAAGTTACACCAGCAAGAGATGTGTCTCGGCACTTTGAGGACACTAGTTACGGC TACAAGGATTTCTCCAGACACGGGATGCATGTCCCAACGTTTCGTGTCCAGGACTACTGCTGGGAAGACC ATGGTTACTCTCTGGTGAATCGTCTCTATCCAGATGTGGGACAGTTAATTGATGAGAAATTTCACATTCC TTACAATCTAACTTACAATACTATGGCCATGCACAAAGATGTTGATACCTCAATGCTCAGACGGGCTATC TGGAACTATATCCACTGCATGTTTGGAATACGATACGACGACTATGACTATGGGGAAATTAACCAGCTGC TGGATCGTAGCTTTAAAGTTTACATCAAAACCGTCGTGTGCACTCCTGAAAAGGTTACCAAAAGGATGTA TGACAGCTTCTGGAGGCAGTTCAAGCACTCTGAGAAGGTCCATGTTAATCTGCTTCTTATAGAAGCTAGG ATGCAAGCAGAACTCCTGTATGCTCTGAGAGCCATTACCCGGTATATGACCTGATGCCTCTCCTCCATTA AAGATGACCCTGGAATGAACAGCACATTTAGTGTACAAGGGGATGGAACCAAGAGCCCCAGGACCAACAG TAGATAAAAAGCATTCAAAAATCCATGTGCCACAATTCCTTTAGTGTCTGCTATTTTACTGTGGGATCTC ACTGCCTGTCTAGCAGTGAGCACTCGGCAGCTTTGGTGTTAGAGCTGGGAAGAAAGGCTGCCGGCCACAG TGTTTGCTTTTTGACAGACAGCACGAGATGCTGTGAACTTGTTTCAATACAGCAACTAGTAAATCTCCAG ATCCCATTGCTCTGATGTGACAAGGTGACAAGACAGAGTGCGTAAAGACTGGGACGTGGCTACTAAGTTC ACACATGCGGAATTGAGTGGCGCTCGGTGCTGCTGTGCGGGGATGAGCACATGGCTATTTGGCTGAGTGC TGCTACCACCTGAGCCCACGCCACACCTTGTGTGCTTTTGAATTGAACTCATGCGCATTATTTTCTTGCA TCTAAAAAAGCATAGAATTTTTACTCACAGCTCAGTCAAGACCGTGTTGGCGGTAACAGTTCATTTCTTT CATTATAGTTTTTAAAATTCAGTTATTGGATGATATTGCAATGACATTCTTAAAAGATTTCCTCCAAAGA GCAATCTTAAAATGATAGTGTGTTAATTATAAGGAAAACAGGCTATCTTTTGATGTGCGTTTCCCAATAT TCTCTGAGCCTGGAGGACAGCTGTCTTGTGCGGGATTTTTGGTTCCGGTGCCAACAATGTGGAAAATGTG GTTGCAGTCTGCTGCACAGGGAGTGCCAGGTGCATTCTGCCCCATGCCCTGCTGACCTCGGGACCCTGAC TTTGAAGGTTATACAGCTCCTTAAAGAATAAAGCTGGAAAATATTTTAGTGAAGTTATCATATTTGTATC TTGATTTACAAAAATGCTAACTTTGAGATACAAACAGGTTTGGAAAAAAATGTATTAAGTACTTTGTATT CTGGAACCGTAAATTGATTTTGAAGTCTGTCAGTATTACTGGTATTTTTCAATAAAGAGTAATTTCCACA AAAAAAAAAAAAAAAAAA |
| Restriction Sites | AscI-NotI |
| ACCN | NM_001013370 |
| Insert Size | 1479 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC055753, AAH55753 |
| RefSeq Size | 2608 bp |
| RefSeq ORF | 1479 bp |
| Locus ID | 140742 |
| UniProt ID | P58006 |
| Gene Summary | Functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling. Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway (PubMed:25259925). This stress-inducible metabolic regulator may also play a role in protection against oxidative and genotoxic stresses. May positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1. May have an alkylhydroperoxide reductase activity born by the N-terminal domain of the protein. Was originally reported to contribute to oxidative stress resistance by reducing PRDX1. However, this could not be confirmed (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG207897 | Sesn1 (tGFP-tagged) - Mouse sestrin 1 (Sesn1) |
CNY 3,710.00 |
|
| MR207897 | Sesn1 (Myc-DDK-tagged) - Mouse sestrin 1 (Sesn1), transcript variant 2 |
CNY 3,656.00 |
|
| MR207897L3 | Lenti ORF clone of Sesn1 (Myc-DDK-tagged) - Mouse sestrin 1 (Sesn1), transcript variant 2 |
CNY 5,230.00 |
|
| MR207897L4 | Lenti ORF clone of Sesn1 (mGFP-tagged) - Mouse sestrin 1 (Sesn1), transcript variant 2 |
CNY 5,230.00 |
