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Atg4d (NM_153583) Mouse Untagged Clone
CAT＃: MC203381
Atg4d (untagged) - Mouse autophagy-related 4D (yeast) (Atg4d), (10ug)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 9830134P12Rik; APG4-D; Apg4d; Atg4dl; Autl4 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC069851
GGGAGCCCGGGTATCCGCTGCGGCCAGAGCCGGACCCGGGAGCCCGGCTCTGAGTATAGAGTAACACTGT GCTGGCCGCGGGGCTGCTCCCGAGCGTTCGCAGTTCCCCGGACCTCGGGGACACCTGCGTCCTTCCTCTC CGGCGGGTCCATGAACTCCGTGTCGCCGGCTGCGGCGCAGTACCGCAGCGGGAGCTCGGAGGACGCGCGC CGGGCCGATTGTCGCCGGCCGAGGGGACAAACCCGTATCCCGGATCCTAGCAACCTGGGACCTTCGGGGT CCGGAGTGGCAGCTCTCGGTTCATCTGGGACAGACCCCGCCGAGCCCGACGAGGTGGACAAGTTCAAAGC CAAGTTCCTGACAGCCTGGAACAACGTCAAGTATGGTTGGGCAGTTAAAAGCCGGACCAGCTTCAGCAAG ATCTCCACTGTTCACCTCTGCGGGCGATGCTACCATTTCGAGGGAGAGGGTGACATCCAGCGGTTTCAAA GGGACTTTGTATCTCGACTATGGCTCACATATCGCCGGGACTTCCCACCCCTTGCTGGAGGCAGCCTGAC CTCAGACTGTGGCTGGGGATGTATGTTACGAAGTGGTCAGATGATGCTGGCCCAGGGCCTGCTCCTGCAT TTTCTGCCTAGAGACTGGAGGTGGGTGGAAGGCACAGGCCTGGCTTCCTCTGAGATGCCAGGGCCAGCCT CTCCCAGTCGCTGCCGAGGGCCTGGTCGTCGTGGGCCCCCAAGATGGACACAGGGTGCACTGGAGATGGA GCAGGACCGTTGGCACCGGCGGATTGTTTCCTGGTTCGCTGATCATCCACGGGCCCCCTTCGGTCTACAT CGGCTAGTGGAGCTTGGGCGGAGCTCGGGCAAGAAGGCAGGAGACTGGTACGGGCCCTCTGTGGTGGCAC ACATCCTCAGGAAAGCTGTGGAGAGTTGCTCAGAAGTCAGCCGCCTGGTGGTGTACGTCTCTCAGGACTG CACAGTGTACAAGGCAGATGTAGCACGCCTGCTGTCCTGGCCAGATCCTACAGCGGAGTGGAAATCTGTG GTCATCCTGGTGCCTGTACGACTGGGCGGGGAGACCCTTAACCCTGTGTACGTGCCCTGTGTGAAGGAGC TTCTGCGTTCAGAACTCTGTCTGGGCATCATGGGCGGCAAGCCCCGGCATTCACTGTACTTCATTGGCTA TCAGGATGACTTTCTGCTCTACCTGGATCCTCACTACTGCCAGCCCACAGTGGATGTCAGCCAGCCCAGC TTCCCCCTGGAGTCCTTTCACTGCACCTCGCCCCGCAAGATGGCCTTTGCCAAGATGGACCCCAGCTGTA CCGTGGGGTTCTATGCTGGGAACAGGAAGGAGTTTGAGACACTCTGCTCGGAGCTGATGAGGATCCTCAG CTCCTCCTCAGTCACGGAACGGTACCCCATGTTCACAGTGGCTGAAGGCCACGCTCAGGACCACAGCCTG GATGCCCTCTGCACCCAGCTGTCCCAGCCCACACTGCGGCTACCTTGCACAGGACGACTACTCAAGGCCA AAAGGCCCAGCTCCGAGGACTTTGTGTTTTTATAATGGGAAGGGATGGGGAAAGATGCCAGACTATTTAT TTTTTTATTTATGTCATGTCAGGTATGGGATCTGCAACTCCAGTGGTGGTGCTGGACCCCTGTCCTCATG TCCCCGTCCTCCCCAACACAGCTGAGACCTATCTGGGCCCCCAGCTGCCCCTGCCTCCTCAGGAGCCAGG GAAGGAGAGTGGGCTCCAGGGCACTCACTCAGGGCCTAATTCAAGTTCTGTAGCCTGCATTGGGTCACTG GTCCCAAGGTCCCAAAGGTCCTGTCCTGGGAGAACTGACTGTGGCTGATAAAGATGCAGACTTGAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_153583 |
| Insert Size | 1425 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC069851, AAH69851 |
| RefSeq Size | 1924 bp |
| RefSeq ORF | 1425 bp |
| Locus ID | 235040 |
| UniProt ID | Q8BGV9 |
| Gene Summary | Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG207595 | Atg4d (tGFP-tagged) - Mouse autophagy-related 4D (yeast) (Atg4d) |
CNY 4,560.00 |
|
| MR207595 | Atg4d (Myc-DDK-tagged) - Mouse autophagy-related 4D (yeast) (Atg4d) |
CNY 3,656.00 |
|
| MR207595L3 | Lenti ORF clone of Atg4d (Myc-DDK-tagged) - Mouse autophagy-related 4D (yeast) (Atg4d) |
CNY 6,080.00 |
|
| MR207595L4 | Lenti ORF clone of Atg4d (mGFP-tagged) - Mouse autophagy-related 4D (yeast) (Atg4d) |
CNY 6,080.00 |
