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Dcun1d5 (NM_029775) Mouse Untagged Clone
CAT＃: MC203127
Dcun1d5 (untagged) - Mouse DCN1, defective in cullin neddylation 1, domain containing 5 (S. cerevisiae) (Dcun1d5), (10ug)
CNY 2,400.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 3110001A18Rik; 4833420K19Rik; AW060460; D430047L21Rik |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC020089
TAGGTGCCGCGCTGTCGGCGTTCGCTGCTCCTGGAAAAGCCCGAGCCGCGACACCGCCGGCCTTAGCGCT GCGCTGCCCGGAACCGCCCCTAAAGAGGAGACCCGGGGCGGGGTCGCGCCTCGGGCCTCCGCTGCGGTCC CTGGCAGGCGGGAGCCGGCGCTGCGGGGCCTGCGGCGGAGCGCGATACCCTTCCCCGGCCGCCCTGTCTA CCTTGCCGGGGAAGGCGGAAGATGCCAGTGAAGAAGAAGAGAAAAGCTCCCGGAGTGGCAGCGGCGGTCG CGGAAGACGCGGGCCTCAAAAAGTGTAAAATCCCCAGCTATTGCAGATCCCAGCCTCCTGCTAGACTCAT CAGTGGAGAGGAAGATTTTTCAAGAAAGAAGTGCCTGGCTTGGTTTTATGAGTATGCAGGTCCTGATGAA GTTGTAGGGCCAGAAGGAATGGAAAAATTTTGTGAAGACATTGGTGTTGAACCTGAAAACATTATTATGT TAGTTTTAGCATGGAAATTGGAGGCTGAAAGCATGGGATTTTTTACCAAGGAAGAATGGTTAAAGGGAAT GACCTCATTACAGTGTGACTGCACAGAAAAGTTACAGAGCAGGTTTGATTTTCTGCGCTCTCAGCTGAAT GACATCTCATCTTTTAAGAATATCTACAGATATGCCTTTGATTTTGCAAGGGATAAAGATCAGAGAAGCC TTGATATTGATACTGCGAAGTCTATGTTAGCTCTGCTGCTTGGGAGAACATGGCCACTCTTTTCAGTATT TTACCAGTACCTGGAGCAATCAAAGTATCGTGTTATGAACAAAGATCAGTGGTACAATGTATTAGAATTC AGCAGAACAGTTCATGCCGATCTTAGTAACTACGATGAAGACGGTGCTTGGCCTGTTCTTCTTGATGAAT TTGTTGAGTGGCAAAAAATCCGTCAAACATCATAGCAAGAACTGTCGTGAAGACAATGCAAACCTTGTGA AGACAATGCAGAGAGGAGGCAGAGTACAGAAAGGGTGCATTTTCACTCACGAAAGACTTCTCATAGTACT TCTTTTCCTTTTTTTTTAAAGGAAGTTTTCTTGTTACATGTGATGGGCATTGAGCCACATGTCTTCTGAG ACTGAATATTGACATTTTTGTTTCAAGTTATGTTTTTAACATTTATTTCAGAACAATAAAGATTCAGATT TGTGCCAAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_029775 |
| Insert Size | 714 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC020089, AAH20089 |
| RefSeq Size | 1233 bp |
| RefSeq ORF | 714 bp |
| Locus ID | 76863 |
| UniProt ID | Q9CXV9 |
| Gene Summary | Contributes to the neddylation of all cullins by transfering NEDD8 from N-terminally acetylated NEDD8-conjugating E2s enzyme to different cullin C-terminal domain-RBX complexes which is necessary for the activation of cullin-RING E3 ubiquitin ligases (CRLs). May play a role in DNA damage response and may participate to cell proliferation and anchorage-independent cell growth.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) contains an alternate exon in the 3' coding sequence compared to variant 1, that causes a frameshift. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG202903 | Dcun1d5 (tGFP-tagged) - Mouse DCN1, defective in cullin neddylation 1, domain containing 5 (S. cerevisiae) (Dcun1d5) |
CNY 2,850.00 |
|
| MR202903 | Dcun1d5 (Myc-DDK-tagged) - Mouse DCN1, defective in cullin neddylation 1, domain containing 5 (S. cerevisiae) (Dcun1d5) |
CNY 2,400.00 |
|
| MR202903L3 | Lenti ORF clone of Dcun1d5 (Myc-DDK-tagged) - Mouse DCN1, defective in cullin neddylation 1, domain containing 5 (S. cerevisiae) (Dcun1d5) |
CNY 4,750.00 |
|
| MR202903L4 | Lenti ORF clone of Dcun1d5 (mGFP-tagged) - Mouse DCN1, defective in cullin neddylation 1, domain containing 5 (S. cerevisiae) (Dcun1d5) |
CNY 4,750.00 |
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